朱砂叶螨的抗药性选育及其解毒酶活性研究

何林; 谭仕禄; 曹小芳; 赵志模; 邓新平; 王进军

朱砂叶螨的抗药性选育及其解毒酶活性研究

1.
西南农业大学植物保护学院,重庆市昆虫学及害虫控制工程重点实验室,重庆 400716

基金项目:

国家自然科学基金资助项目(39970493);重庆市科技攻关资助项目(6606).

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出版历程

Study on Resistance Selection and Activity of Detoxifieation Enzyme in Tetranychus cinnabarinus (Boiduval)

1.
Key Laboratory of Entomology and Pest Control Engineering of Chongqing,Southwest Agricultural University, Chongqing 400716,China

摘要

摘要: 在室内模拟田间药剂的选择压力,用阿维菌素和甲氰菊酯对朱砂叶螨Tetranychus cinnabarinus 进行逐代处理,以选育其抗药性品系。阿维菌素品系选育至42代,抗性增长到8.7倍,甲氰菊酯品系选育至40代,抗性增长到68.5倍。阿维菌素抗性品系羧酸酯酶(CarE)、谷胱甘肽-S-转移酶(GSTs)、多功能氧化酶(MFO)的活性分别为敏感品系的2.7、3.4和1.4倍,差异达显著水平。推测3种解毒酶活性显著升高是朱砂叶螨对阿维菌素产生抗性的重要原因。甲氰菊酯抗性品系GSTs的活性为敏感品系的2.8倍,差异显著,表明该抗性品系的形成与GSTs活性增强有关。羧酸酯酶动力学测定结果表明,朱砂叶螨阿维菌素抗性品系体内存在变构的羧酸酯酶。
- 朱砂叶螨 /
- 阿维菌素 /
- 甲氰菊酯 /
- 抗药性 /
- 解毒酶
Abstract: Fenpropathrin and abamectin were used to select the resistance in Tetranychus cinnabarinus in laboratory. After 42 and 40 generations selection with abamectin and fenpropathrin, Tetranychus cinnabarinus developed 8.7 and 68.5-fold resistance to the two acaricides respectively. The major resistant mechanism to abamectin was the increasing of the activity of carboxylesterases (CarE), glutathion S-transferase (GSTs) and mixed function oxidase (MFO), and the activity in resistant strain developed 2.7, 3.4 and 1.4-fold contrasted to susceptible strain respectively. The activity of GSTs in the strain selected with fenpropathrin developed 2.8 fold contrast to susceptible strain, which meant the resistance was related with the increasing of the activity of GSTs in resistant strain. The result of the kinetic determination of CarE showed that the structure of CarE in the strain selected with abamectin has been changed.
- Tetranychus cinnabarinus /
- abamectin /
- fenpropathrin /
- resistance /
- detoxifieation enzyme

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