印楝素和阿维菌素的液相色谱法同时测定
Simultaneous Determination of Azadirachtin and Avermectin B1a by Reverse Phase HPLC
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摘要: 提出了一种可同时测定印楝素·阿维菌素乳油中印楝素和阿维菌素B1a的液相色谱法。该方法使用Hypersil ODS C18(250 mm×4 mm,5 μm)色谱柱和紫外检测器(印楝素λ 215 nm,阿维菌素B1a λ 240 nm),印楝素和阿维菌素B1a的回收率分别为97.5%~105.0% 和98.0%~107.5%,相对标准偏差分别为1.70%和1.60%,达到了简便、快速、准确的目的。Abstract: A reverse phase high performance liquid chromatographic method for the simultaneous determination of azadirachtin and avermectin B1a in mixed emulsifiable concentrate (EC) was developed. The procedure adopted a C18 column(Hypersil ODS 250 mm×4 mm) with UV detection at λ 215 nm(azadirachtin) and λ 240 nm(avermectin B1a). The recoveries of azadirachtin ranged from 97.5% to 105.0%, and avermectin B1a, from 98.0% to 107.5%. The relative standard deviation of azadirachtin and avermectin B1a were 1.70% and 1.60% respectively. The proposed method achieved the goal of simplicity, rapidity and accuracy.
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Key words:
- HPLC /
- azadirachtin /
- avermectin B1a
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Maienfisch P, Angst M, Brandl F, et al. Chemistry and biology of thiamethoxam: a second generation neonicotinoid [J]. Pest Manag Sci, 2001, 57 (10): 906-913. Maienfisch P, Huerlimann H, Rindlishbacher A, et al. The discovery of thiamethoxam: a second-generation neonicotinoid [J]. Pest Manag Sci, 2001, 57 (2): 165-176. 农业部农药检定所. 农药登记公告汇编 [M]. 北京: 中国农业大学出版社, 2001. 65. Cahill M, Gorman K, Day S, et al. Baseline determination and detection of resistance to imidacloprid in Bemisia tabaci (Homoptera: Aleyrodidae) [J]. Bull Entomol Res, 1996, 86: 343-349. Tomizawa M, Casida J E. Selection toxicity of neonicotinoids attributable to specificity of insect and mammalian receptors [J]. Annu Rev Entomol, 2003, 48: 339-364. -
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