浙江省二化螟不同种群和大螟 对三唑磷的敏感性研究

黄诚华; 姚洪渭; 叶恭银; 蒋学辉; 胡萃; 程家安

浙江省二化螟不同种群和大螟对三唑磷的敏感性研究

1.
浙江大学水稻生物学国家重点实验室,应用昆虫学研究所,浙江杭州 310029
2.
浙江省植物保护总站,浙江杭州 310020

基金项目:

国家自然科学基金重点项目(30230070);浙江省农业厅水稻重大病虫害防治项目

计量
- 文章访问数: 1127
- HTML全文浏览量: 29
- PDF下载量: 490
- 被引次数: 0
出版历程
- 收稿日期: 2005-05-10
- 修回日期: 2005-09-01

Susceptibility of Different Populations of Chilo suppressalis and Sesamia inferens to Triazophos in Zhejiang Province of China

1.
State Key Laboratory of Rice Biology, Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China
2.
General Station of Plant Protection Zhejiang Province, Hangzhou 310020, China

摘要

摘要: 于2004年用点滴法测定了浙江杭州地区(富阳和余杭)和嘉兴地区(平湖、秀城和秀洲)二化螟、杭州富阳大螟等种群4龄幼虫对三唑磷的敏感性,以及各种群体内与抗药性相关酶系(多功能氧化酶、谷胱甘肽S-转移酶、酯酶和乙酰胆碱酯酶等)的比活力。结果证实上述地区二化螟种群对三唑磷产生了21.1~218.8倍的抗性,其中以富阳和平湖种群的抗性水平较高。二化螟体内多功能氧化酶活性与三唑磷抗性水平呈显著正相关,而乙酰胆碱酯酶活性则呈显著负相关,表明二化螟对三唑磷的抗性可能与多功能氧化酶活性增强和乙酰胆碱酯酶敏感性降低有关。大螟富阳种群对三唑磷的敏感性明显高于二化螟富阳种群,其抗性机制还有待研究。
- 二化螟 /
- 大螟 /
- 三唑磷 /
- 抗药性 /
- 酶活性
Abstract: In 2004, the susceptibility to triazophos of striped stem borer (SSB), Chilo suppressalis (Walker) populations collected from Hangzhou district (Fuyang and Yuhang) and Jiaxin district (Pinghu, Xiucheng and Xiuzhou) of Zhejiang Province as well as pink stem borer (PSB), Sesamia inferens (Walker) population collected from Fuyang were assayed, respectively. Meanwhile, the activities of 4 enzymes related to the insecticide resistance including mixed function oxidase, glutathione S-transferase, esterase and acetylcholinesterase were determined. By comparison with a susceptible strain, there was a significant increase of 21.1- to 218.8- folds in LD50 value to triazophos in all SSB populations tested. SSB from Fuyang and Pinghu performed less sensitive to triazophos than that from Xiuzhou, Xiucheng and Yuhang. The results showed that the resistance mechanism to triazophos in SSB tested was related to increase of mixed function oxidase activity and insensitivity of acetylcholinesterase. PSB was more susceptible to triazophos than SSB from the same location, Fuyang, however, its resistance mechanism to triazophos need to be further studied.
- Chilo suppressalis /
- Sesamia inferens /
- triazophos /
- insecticide resistance /
- enzyme activity

HTML全文

参考文献(28)

SHENG Cheng-fa(盛承发),XUAN Wei-jian(宣维健),JIAO Xiao-guo(焦晓国),et al.我国稻螟暴发成灾的原因、趋势及对策[J].J Natural Disasters(自然灾害学报),2002,11(3):103-108.

SHENG Cheng-fa(盛承发),WANG Hong-tuo(王红托),SHENG Shi-yu(盛世余),et al.我国稻螟灾害的现状及损失估计[J].Entomol Knowl(昆虫知识),2003,40(4):289-294.

WANG Qiang(王强),TAN Fu-jie(谭福杰),YOU Zi-ping(尤子平).二化螟对六类杀虫剂的耐药性及增效剂的增效作用研究[J].J Nanjing Agric Univ(南京农业大学学报),1987,4(Suppl):44-55.

TANG Zhen-hua(唐振华).Insect Resistance to Insecticides and It's Management(昆虫抗药性及其治理)[M].Beijing(北京):China Agriculture Press(中国农业出版社),1993.

CHU Bai(褚柏),SHU Jian-kun(苏建坤),ZHU Jing-qing(朱锦清),et al.六省(市)水稻二化螟对常用杀虫剂敏感度的调查[J].Pesticides(农药),1990,29(6):6-8.

XIAO Gui-zhang(肖桂章),WEN Cheng-zhi(文承志),PI Pi-deng(皮丕登),et al.湘北地区三化螟种群消长的原因探讨[J].Entomol Knowl(昆虫知识),2000,37(6):321-324.

ZHAO Xue-ping(赵学平),WANGQiang(王强),WU Chang-xing(吴长兴),et al.二化螟对杀虫剂的敏感性及抗药性研究[J].ActaAgricZhejiang(浙江农业学报),2000,12(6):382-386.

HU Shi-meng(胡仕孟),XIE Shi-jie(谢士杰),XU Shan-gang(徐善刚),et al.浙江慈溪水稻二化螟抗药性研究初报[J].ActaAgricZhejiang(浙江农业学报),2001,13(1):38-41.

JIANG Xue-hui(蒋学辉),ZHANG Qiang-hua(章强华),HU Shi-meng(胡仕孟),et al.浙江省水稻二化螟抗药性现状与治理对策[J].Plang Protec Technol Exten(植保技术与推广),2001, 21(3):27-29.

CAO Ming-zhang(曹明章),SHEN Jin-liang(沈晋良),ZHANG Shao-ming(张绍明),et al.2002 年江苏省二化螟抗药性检测及治理[J].Plant Protec(植物保护),2003,29(5):34-37.

CAO Ming-zhang(曹明章),SHEN Jin-liang(沈晋良),ZHANG Jin-zhen(张金振),et al.二化螟抗药性监测和对三唑磷抗性的遗传分析[J].Chin J Rice Sci(中国水稻科学),2004,18(1):73-79.

SHANG Zhi-zhen(尚稚珍),WANG Yin-shu(王银淑),ZOU Yong-hua(邹永华).二化螟饲养方法的研究[J].Acta Entomol Sinica(昆虫学报),1979,22(2):164-167.

FAO. Method for larvae of the rice stem borer (Chilo suppressalis Walker) -FAO Method NO.3 . FAO. Pest Resistance to Pesticides and Crop Loss Assessment-2[Z]. Rome, 1980. 25-28.

Yang Y, Wu Y, Chen S, et al. The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia[J]. Insect Biochem Mol Biol, 2004, 34: 763-773.

Oppenoorth F J, van der Pas L J R, Houx N W H. Glutathione-S-transferase and hydrolytic activity in a tetrachlorvinphos-resistance strain of housefly and their influence on resistance[J]. Pestic Biochem Physiol, 1979, 11: 176-188.

Hama H, Hosoda A. High aliesterase activity and low acetylcholinesterase sensitivity involved in organophosphorus and carbamate resistance of the brown planthopper[J]. Appl Entomol Zool, 1983, 18(4): 475- 485.

GAO Xi-wu(高希武).Groun等改进的Ellman胆碱酯酶活性测定方法介绍[J].Entomol Knowl(昆虫知识),1987,24(4):245- 246.

Bradford M M. A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Anaytical Biochem, 1976, 72:248-254.

SAS Institute Inc. SAS/STAT User's Guide . Version 6, Cary, NC: SAS Institute Inc. 1996.

LeOra. POLO-PC: A User's Guide to Probit or Logit Analysis . LeOra Software, Berkeley, CA, 1997.

Konno Y, Domon K. Fenitrothion resistance and carboxylesterase isozyme pattern in the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae), collected from Yamagata Prefecture[J]. Annual Report of the Society of Plant Protection of North Japan, 1998, 49: 105-108.

PENG Yu(彭宇),CHEN Chang-kun(陈长琨),HAN Zhao-jun(韩召军),et al.江苏省水稻二化螟的抗药性测定及对甲胺磷的抗性机制[J].Acta Phytophyl Sinica(植物保护学报),2001,28(2):173-177.

HAN Zhao-jiu(韩招久),HAN Zhao-jun(韩召军),WANG Yin-chang(王荫长),et al.二化螟抗杀虫单和甲胺磷品系的生化特性[J].Acta Entomol Sinica(昆虫学报),2003,46(2):161-170.

Qu M, Han Z, Xu X, et al. Triazophos resistance mechanisms in the rice stem borer (Chilo suppressalis Walker)[J]. Pestic Biochem Physiol, 2003, 77(3):99-105.

HUANG Ze-wei(黄泽威),WANG Dong-ming(王东明).水稻螟虫群体变化趋势分析及防治对策探讨[J].Jiangshu Agric Sci(江苏农业科学),2001,(1):39- 41.

LI Hong-shan(李洪山),LI Ci-hou(李慈厚).大螟治理对策及其化防药剂选择[J].Rice China(中国稻米),2003,(3):34.

TANG Fu-bin(汤富彬),HU Guo-wen(胡国文).50%锐·乙酰可湿性粉剂防治二化螟、稻飞虱、稻纵卷叶螟药效[J].Pesticides(农药),2002,41(11):25-27.

ZHU Chun-qiang(祝春强),LIU Ming-xi(刘明熙),WEI Yong-bao(韦永保),et al.氟虫腈和三唑磷混用防治单季稻主要害虫的效果与技术[J].Anhui Agric Sci(安徽农业科学),2002,30(1):57-59. (Ed.TANG J)

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 1127
HTML全文浏览量: 29
PDF下载量: 490
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板