同源与异源分析对ELISA检测灵敏度和特异性的影响

施海燕; 朱国念; 郑尊涛; 沈晋良

同源与异源分析对ELISA检测灵敏度和特异性的影响

1.
浙江大学农药与环境毒理研究所,浙江杭州 310029
2.
南京农业大学农药系,农业部病虫监测与治理重点开放实验室,江苏南京 210095
3.
农业部农药检定所,北京100026

基金项目:

国家自然科学基金(30390944)资助项目. 同源分析、异源分析进行了研究,并对识别机制问题作了初步探讨。

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出版历程
- 收稿日期: 2005-04-05
- 修回日期: 2005-08-30

Effect of Homologous Assay and Heterologous Assay on the ELISA Detective Sensitivity and Specificity

1.
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029,China
2.
Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, Department of Pesticide Science, Nanjing Agricultural University, Nanjing 210095,China
3.
Institute for Control of Agrochemical, Ministry of Agriculture, Beijing 100026,China

摘要

摘要: 以获得的抗2甲4氯抗体及其包被抗原为研究对象,比较了同源和异源分析的灵敏度和特异性,并试图进一步揭示抗体的识别机制。结果表明:异源分析,特别是半抗原结构异源可大幅度提高检测灵敏度;同源分析和异源分析对于类似化合物的特异性无明显差异。
- 同源分析 /
- 异源分析 /
- 灵敏度 /
- 特异性
Abstract: The detective sensitivity and specificity of homologous assay were compared with heterologous assay on MCPA. And the recognition mechanism of antibody was discribed. The result showed that heterologous assay, especially heterology in structure of hapten would largely improve the sensitivity of ELISA assay. And there was no efficiency difference in specificity for the analogous compound.
- homologous assay /
- heterologous assay /
- sensitivity /
- specificity

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参考文献(7)

Wie S I, Hammock B D. Comparison of coating and immunizing antigen structure on the sensitivity and specificity of immunoassays for benzoylphenylurea insecticides[J]. J Agric Food Chem,1984,32:1294-1301.

Schneider P, Hammock B D. Influence of the ELISA format and the hapten enzyme conjugate on the sensitivity of an immunoassay for S-triazine herbicides using monoclonal antibodies[J]. J Agric Food Chem,1992, 40: 525-530.

Kim Y J, Cho Y A, Hammock B D, et al. Synthesis of haptens for immunoassay of organophosphorus pesticides and effect of heterology in hapten spacer arm length on immunoassay sensitivity[J]. Analytica Chimica Acta,2003,475:85-96.

Gerdes M, Meusel M, Spener F. Development of a displacement immunoassay by exploiting cross-reactivity of a monoclonal antibody[J]. Anal Biochem, 1997,252:198-204.

Dunbar B, Riggle B, Niswender G. Development of enzyme immunoassay for the detection of triazine herbicides[J]. J Agric Food Chem,1990, 38: 433-437.

Gooddrow M H, Harrison R O, Hammock B D. Competitive inhibition ELISA for the S-triazine herbicides:Assay optimization and antibody characterization[J]. J Agric Food Chem,1991,39(1):122-128.

SHI Hai-yan(施海燕), WU Hui-ming(吴慧明), CHENG Jing-li(程敬丽), et al. 2甲4氯人工抗原的合成与鉴定[J]. Chin J Pestic Sci(农药学学报), 2004, 6(2): 20-24. (Ed.JIN S H)

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文章访问数: 1318
HTML全文浏览量: 64
PDF下载量: 517
被引次数: 0

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