不同温度下纳他霉素对灰葡萄孢的抑制活性和作用机理初探

张鹏; 姜兴印; 房锋; 纪春涛; 张培正; 李大鹏

不同温度下纳他霉素对灰葡萄孢的抑制活性和作用机理初探

1.
山东农业大学植物保护学院, 山东泰安 271018
2.
山东农业大学食品科学与工程学院, 山东泰安 271018

基金项目:

国家"十一五"科技支撑计划课题 (2006BAK02A23).

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出版历程
- 收稿日期: 2007-09-11

Primary Studies on Inhibitory Activity of Natamycin to Botrytis cinereaunder Different Temperatures and its Action Mechanism

1.
College of Plant Protection,Shandong Agricultural University,Tai'an271018,Shandong Province,China
2.
College of Food Science and Engineering,Shandong Agricultural University,Tai'an271018,Shandong Province,China

摘要

摘要: 采用菌丝生长速率法测定了不同温度下纳他霉素和乙霉威对灰葡萄孢Botrytis cinerea的抑制活性,同时采用药液培养法间接测定了不同温度下药剂对菌丝体糖、蛋白和电解质渗漏的影响。结果表明:纳他霉素对菌丝生长的抑制中浓度(EC50)在25℃下为37.42 μg/mL,在5℃下为0.984 0 μg/mL,活性提高了37倍;乙霉威对供试菌的活性在5℃下较25℃提高了5倍。25℃下经纳他霉素处理8 h后,菌丝体可溶性糖渗漏量为6.5 mg/g,蛋白渗漏量为162 μg/g,药液电导率为700 μs/cm,均显著高于相同条件下的0.1%吐温-80对照和乙霉威处理。不同处理温度之间,温度越低菌丝内含物的渗漏量越少。
- 纳他霉素 /
- 灰葡萄孢 /
- 抑制作用 /
- 作用机理
Abstract: Inhibitory activity of natamycin and diethofencarb to Botrytis cinerea Pers.under different temperatures was tested by mycelium growth rate method.Mycelial leakage of sugar,protein and electrolyte was tested by liquid cultivate method indirectly.The results showed that EC50 values of natamycin were 37.42 μg/mL in 25℃,and 0.984 0 μg/mL in 5℃,increased 37-fold.The activity of diethofencarb to B.cinerea increased 5-fold.Eight hours after treated by natamycin,the sugar leakage was 6.5 mg/g,the protein leakage was 162 μg/g and the conductance was 700 μs/cm.All of these were higher than that treated by 0.1% tween-80(CK) and diethofencarb.The mycelial leakage decreased as the temperature fell down.
- natamycin /
- Botrytis cinerea /
- inhibitory activity /
- action mechanism

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参考文献(17)

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