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多杀菌素亚致死浓度对小菜蛾解毒酶系活力的影响

尹显慧 吴青君 李学锋 张友军 徐宝云

尹显慧, 吴青君, 李学锋, 张友军, 徐宝云. 多杀菌素亚致死浓度对小菜蛾解毒酶系活力的影响[J]. 农药学学报, 2008, 10(1): 28-34.
引用本文: 尹显慧, 吴青君, 李学锋, 张友军, 徐宝云. 多杀菌素亚致死浓度对小菜蛾解毒酶系活力的影响[J]. 农药学学报, 2008, 10(1): 28-34.
YIN Xian-hui, WU Qing-jun, LI Xue-feng, ZHANG You-jun, XU Bao-yun. Effect of Sublethal Concentrations of Spinosad on the Activities of DetoxifyingEnzymes in the Larvae of Diamondback Moth Plutella xylostell[J]. Chinese Journal of Pesticide Science, 2008, 10(1): 28-34.
Citation: YIN Xian-hui, WU Qing-jun, LI Xue-feng, ZHANG You-jun, XU Bao-yun. Effect of Sublethal Concentrations of Spinosad on the Activities of DetoxifyingEnzymes in the Larvae of Diamondback Moth Plutella xylostell[J]. Chinese Journal of Pesticide Science, 2008, 10(1): 28-34.

多杀菌素亚致死浓度对小菜蛾解毒酶系活力的影响

基金项目: 

国家重点基础研究发展规划("973"计划)项目(2006CB102003);国家自然科学基金项目(30171431);国家科技支撑计划项目(2006BAD08A03-3).

Effect of Sublethal Concentrations of Spinosad on the Activities of DetoxifyingEnzymes in the Larvae of Diamondback Moth Plutella xylostell

  • 摘要: 采用多杀菌素亚致死浓度,以浸叶法分别处理小菜蛾Plutella xylostella (L.)敏感种群(SS)和亚致死选育种群 的3龄幼虫,分别测定饲喂处理6、12、24、48和72 h后小菜蛾体内羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GST) 和多功能氧化酶(MFOs)的活性,分析了酶活性的变化动态。结果表明,SS种群小菜蛾CarE的活性在不同时间段波动较大,经多杀菌素处理后,开始时段比活力增加,随着处理时间的延长,比活力逐渐被抑制,Sub-SS种群的GarE活力高于SS种群;多杀菌素对GST具有明显的诱导作用,亚致死浓度处理后GSTs比活力呈上升趋势,且具有一定的时间效应;对细胞色素P450酶系的O-脱甲基酶活性具有明显的抑制作用,多杀菌素亚致死浓度连续处理5代后,该酶活性更低。
  • TALEKAR N S,SHELTON A M.Biology,Ecology and Management of the Diamondback Moth [J].Annu Rev Entomol,1993,38: 275-301.
    PERVEEN F.Sublethal Effects of Chlorfluazuron on Reproductivity and Viability of Spodoptera litura (F.) (Lep.,Noctuidae) [J].J Appl Entomol,2000,124: 5-6,223-231.
    ELZEN G W.Lethal and Sublethal Effects of Insecticide Residues on Orius insidiosus (Hemiptera: Anthocoridae) and Geocoris punctipes (Hemiptera:Lygaeidae) [J].J Econ Entomol,2001,94(1): 55-59.
    GAO Zong-ren (高宗仁),LI Qiao-si(李巧丝),LIU Xiao-chun(刘孝纯).杀虫剂对朱砂叶螨某些生物学特性的影响 [J].Acta Phytophyl Sin (植物保护学报),1991,18(3):283-287.
    NANDIHALLI B S,PATIL B V,HUGAR P.Influence of Synthetic Pyrethroid Usage on Aphid Resurgence in Cotton[J].Karnataka Agric Sci,1992,5(3):234-237.
    DEVORSHAK C,REO R M.The Role of Esterases in Insecticide Resistance[J].Rev Toxic,1998,(2): 501-537.
    DESNEUX N,DECOURTYE A,DELPUECH J M.The Sublethal Effects of Pesticides on Benefical Arthropods [J].Annu Rev Entomol,2007,52: 81-106.
    HYNE R V,MAHER W A.Invertebrate Biomarkers: Links to Toxicosis that Predict Population Decline [J].Ecotoxicol Environ Saf,2003,54: 366-374.
    RUMPF S,HETZEL F,FRAMPTON C.Lacewings (Neuroptera: Hemerobiidae and Chrysopidae) and Integrated Pest Management:Enzyme Activity as Biomarker of Sublethal Insecticide Exposure [J].J Econ Entomol,1997,90 (1): 102-108.
    GAO Xi-wu(高希武),DONG Xiang-li(董向丽),ZHENG Bing-zong(郑炳宗),et al.棉铃虫的谷胱甘肽S-转移酶(GST): 杀虫药剂和植物次生性物质的诱导与GST对杀虫药剂的代谢 [J].Acta Entomologica Sinica(昆虫学报),1997,40(2): 122-125.
    FEYEREISEN R.Cytochrome P450 in Insect [M]//SCKENKMAN J B.Cytochrome P450.Berlin:Springer,1993:311-324.
    GERALD B.WATSON G B,Action of Insecticidal Spinosyns on Gaminbutyric Acid Responses from Small-diameter Cockroach Neurons [J].Pestic Biochem Physiol,2001,71:20-26.
    WANG Yan-hua (王彦华),WANG Ming-hua (王鸣华).多杀菌素的作用机理及其抗药性的研究进展 [J].Pestic Sci Admin(农药科学与管理), 2006,25(11):12-15.
    ZHAO J Z,LI Y X,COLLINS H L,et al.Monitoring and Characterization of Diamondback Moth (Lepidoptera: Plutellidae) Resistance to Spinosad [J].J Econ Entomol,2002,95:430-436.
    HAMA H, HOSODA A.High Aliesterase Activity and Low Acetylcholinesterase Sensitivity Involved in Organophosphorus and Carbamate Resistance of the Brown Planthopper[J].Appl Entomol Zool,1983,18(4):475-485.
    OPPENOORT H F J,VAN DER PAS L J R,HOUX N W H.GIutathione-S-transferase and Hydrolytic Activity in a Tetrachlorvinphos-resistance Strain of Housefly and their Influence on Resistance[J].Pestic Biochem Physiol,1979,11:176-188.
    MU Li-yi (慕立义).Research Methods of Chemical Plant Protection (植物化学保护研究方法)[M].Beijing(北京):China Agriculture Press (中国农业出版社),1994:160-161.
    YU S J,NGUYEN S N.Detection and Biochemical Characterization of Insecticide Resistance in the Diamondback Moth [J].Pestic Biochem Physiol,1992,44 (1):74-81.
    BRADFORD W W.A Rapid and Sensitive Method for the Quantization of Microgram Quantities of Protein Utilization the Principle of Protein-dye Binding [J].Anal Biochem,1976,72: 248-254.
    TANG Zhen-hua (唐振华),BI Qiang (毕强).The Molecular Behavior of Pesticides (杀虫剂作用的分子行为)[M].Shanghai(上海): Shanghai Yuandong Press (上海远东出版社), 2003:58-110.
    TANG Zhen-hua (唐振华). Insecticide Resistance of Insects and its Management (昆虫抗药性及其治理)[M].Beijing (北京):China Agriculture Press (中国农业出版社),1993:212-245.
    ZHANG You-jun (张友军), WANG Guang-feng (王光峰), WU Qing-jun (吴青君),et al.多杀菌素对甜菜夜蛾多酚氧化酶和羧酸酯酶的影响 [J].Chin J Pestic Sci(农药学学报),2003,5(2):40-46.
    XIA Bing (夏冰),SHI Tai (石泰),LIANG Pei(梁沛),et al.杀虫剂亚致死剂量对小菜蛾羧酸酯酶的影响[J].Chin J Pestic Sci(农药学学报), 2002,4(1):23-27.
    CHEN Lie-zhong(陈列忠),WANG Kai-jin(王开金),YU Xiao-ping(俞晓平),et al.雷公藤生物碱对小菜蛾幼虫生长及其解毒酶系的影响 [J].Entomol J East China(华东昆虫学报),2005,14(3): 238-242.
    LIANG Pei(梁沛), XIA Bing(夏冰), GAO Xi-wu(高希武),et al.阿维菌素和高效氯氰菊酯亚致死剂量对小菜蛾谷胱甘肽S - 转移酶的影响 [J].J Chin Agric Univ(中国农业大学学报),2003,8(3):65-68.
    WEI Bo (魏波), HAO Chi (郝赤), LI Hui-xian (李会仙).杀虫剂亚致死剂量对棉铃虫靶标酶的影响 [J].Modern Pesticides(现代农药),2006,5(3):35-38.
    MO J C,WANG W,CHENG J A,et al.Selection and Characterization of Spinosad Resistance in Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)[J].Pestic Biochem Physiol,2006,84:180-187.
    BALABASKARAN S,CHUEN S S,MUNIANDY S.Glutathione S-transferase from the Diamondback Moth Plutella xylostell (L.) [J].Insect Biochem,1989,19 (4):435-443.
    LENG Xin-fu(冷欣夫),TANG Zhen-hua (唐振华),WANG Yin-chang (王荫长).Molecular Toxicology and Insects Insecticide Resistance (杀虫剂分子毒理学及昆虫抗药性) [M].Beijing (北京):China Agriculture Press (中国农业出版社), 1996:134.
    WEI Cun-xu (韦存虚),ZHU Fu-xing (朱福兴),LI Dong-xia (李东霞),et al.氰戊菊酯、辛硫磷及其混剂对棉铃虫解毒代谢酶的影响 [J].J Yangzhou Univ(扬州大学学报),2004,25 (3):59-64.
    WEI Hui (魏辉), LI Hong-shan (李洪山),DAI Hua-guo (戴华国),et al.多杀菌素对小菜蛾体内多功能氧化酶和保幼激素酯酶活力的影响 [J].Chin J Pestic Sci(农药学学报), 2006,8 (3):239-244.
    MA Zhi-qing (马志卿), LI Guang-ze (李广泽), ZHANG Xing (张兴),et al.脱氧鬼臼毒素对粘虫几种代谢酶系的影响 [J].Acta Entomologica Sinica(昆虫学报),2007,50 (2):186-190.
    WANG Juan (王娟),DAI Hua-guo (戴华国),LI Hong-shan (李洪山).印楝素作用下小菜蛾体内MFO和JHE活力的变化 [J].Acta Agric Jiangxi(江西农业学报),2006,18 (4):121-125.
    PILLING E D,BROMLEYCHALLENOR K A C,WALKER C H,et al.Mechanism of Synergism between the Pyrethroid Insecticide lambda-Cyhalothrin and the Imidazole Fungicide Prochloraz,in the Honeybee (Apis mellifera L.) [J].Pestic Biochem Physiol,1995,51:1-11. (Ed.JIN S H)
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  • 收稿日期:  2007-11-03

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