辣椒碱对小菜蛾体内谷胱甘肽S-转移酶和Na+ -K+ -ATP酶活性的影响

刘少武; 纪明山; 谷祖敏; 魏松红; 王英姿

辣椒碱对小菜蛾体内谷胱甘肽S-转移酶和Na+ -K+ -ATP酶活性的影响

刘少武^1,2,,
纪明山^1,2,,
谷祖敏^1,2,
魏松红^1,2,
王英姿^1,2

1.
沈阳农业大学植物保护学院
2.
辽宁省生物农药工程技术研究中心,沈阳 110161

基金项目:

辽宁省"十一五"科技攻关项目(2006215004);辽宁省工程技术研究中心专项(200535).

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出版历程
- 收稿日期: 2007-12-14

Effects of Capsaicin on the Enzyme Activities of Glutathione S-transferase andNa+ -K+ -ATPase in Plutella xylostella

LIU Shao-wu^{1,2
,},
JI Ming-shan^{1,2
,},
GU Zu-min^1,2,
WEI Song-hong^1,2,
WANG Ying-zi^1,2

1.
College of Plant Protection,Shenyang Agricultural University
2.
Biopesticide EngineeringResearch Center of Liaoning Province,Shenyang 110161,China

摘要

摘要: 采用常规生化方法测定了辣椒碱对小菜蛾体内谷胱甘肽S-转移酶和Na+ -K+ -ATP酶活性的影响。结果表明,经不同浓度辣椒碱处理12 h后,小菜蛾体内谷胱甘肽S-转移酶活力均显著低于空白对照,在100.56~141.53 μmol ·L-1 ·mg-1Pro ·min-1 之间;而Na+ -K+ -ATP酶活力均显著高于空白对照,在12.43~20.36 μmol ·mg-1Pro ·min-1之间。说明辣椒碱能抑制小菜蛾体内谷胱甘肽S-转移酶的活性,而对Na+ -K+ -ATP酶活性则表现为促进作用。
- 辣椒碱 /
- 小菜蛾 /
- 谷胱甘肽S-转移酶 /
- Na⁺ -K⁺ -ATP酶
Abstract: The effects of capsaicin on glutathione S-transferase and Na+ -K+ -ATPase of Plutella xylostella (L.) were tested with conventional methods in laboratory.The results showed that compared with the negative control,the activity of glutathione S-transferase decreased significantly after treatment for 12 hours by capsaicin at different concentrations,which ranged from 100.56 to 141.53 μmol ·L-1·mg-1Pro ·min-1.However,the activity of Na+ -K+ -ATPase increased significantly after the same treatment,which ranged from 12.43 to 20.36 μmol ·mg-1Pro ·min-1.It suggested that capsaicin can inhibit the activity of glutathione S-transferase and activate the activity of Na+ -K+ -ATPase.
- capsaicin /
- Plutella xylostella (L.) /
- glutathione S-transferase /
- Na⁺ -K⁺ -ATPase

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