三种植物次生物质对B型烟粉虱羧酸酯酶活性及其对药剂敏感度的影响

张爱萍; 宋敦伦; 史雪岩; 梁沛; 高希武

三种植物次生物质对B型烟粉虱羧酸酯酶活性及其对药剂敏感度的影响

1.
中国农业大学昆虫学系,北京 100193

基金项目:

国家重点基础研究发展规划(973计划)项目(2002CB111407).

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出版历程
- 收稿日期: 2008-03-13

Effects of Three Allelochemcals on the Carboxylesterase Activity and itsSusceptibility to Insecticides in Bemisia tabaci Biotype B

1.
Department of Entomology,China Agricultural University,Beijing 100193,China

摘要

摘要: 采用人工饲料添加法,研究了不同浓度的槲皮素、2-十三烷酮和葫芦素B 3种植物次生物质对B型烟粉虱Bemisia tabaci羧酸酯酶(CarE)活性的影响,同时比较研究了用3种植物次生物质处理后敌敌畏、脱叶磷、灭多威、乐果和氧乐果5种药剂对CarE 的抑制中浓度(I50)的变化。结果表明: 0.01~1.0 mg/mL的槲皮素对B型烟粉虱成虫CarE活性均具有明显的诱导增加作用,最高为对照的4.32倍; 0.1和0.5 mg/mL的2-十三烷酮处理使CarE活性比对照分别下降了22%和58%; 0.75~30.0 mg/L的葫芦素B对烟粉虱CarE活性均表现为抑制作用;用1.0 mg/mL的槲皮素处理24 h后,敌敌畏和脱叶磷对烟粉虱CarE的I50值分别增加为对照的20.05和3.16倍; 3.75 mg/L 的葫芦素B处理后,敌敌畏和灭多威对CarE 的I50值分别增加为对照的25.94、2.45倍; 1.0 mg/mL的2-十三烷酮处理后敌敌畏、脱叶磷和乐果对CarE 的I50值分别降低了89%、83%和90%。
- 烟粉虱 /
- 槲皮素 /
- 2-十三烷酮 /
- 葫芦素B /
- 羧酸酯酶 /
- 杀虫剂
Abstract: The effects of quercetin,2-tridecanone and cucurbitacin B on the specific activity of carboxylesterase (CarE) in the tobacco whitefly,Bemisia tabaci (Gennadius) type B were investigated by feeding on artificial diet.The sensitivities of CarE to dichlorvos,methomyl,dimethoate,omethoate and S,S,S-tributyl phosphorotrithioate(DEF),an inhibitor of CarE,were also determined.After treated with quercetin ranged from 0.01 mg/mL to 1.0 mg/mL for 24 h,the specific activity of CarE increased obviously and the highest was 4.32-fold higher than the control. The specific activity of CarE was partially inhibited by 22% and 58%,respectively in the 0.1 and 0.5 mg/mL 2-tridecanone treatments. The cucurbitacin B ranged from 0.75 to 30.0 mg/L inhibited activity of CarE obviously.The I50 values of dichlorvos and DEF to the CarE from B.tabaci treated with 1.0 mg/L quercetin were respectively 20.05- and 3.16-fold higher than the control. When treated with 3.75 mg/L of cucurbitacin B for 24 h,the I50 values of dichlorvos and methomyl to the CarE were 25.94- and 2.45- fold higher than the control, respectively.While when treated with 1.0 mg/mL 2-tridecanone for 24 h,the I50 values of dichlorvos,DEF and dimethoate to the CarE were decreased by 89% ,83% and 90% ,respectively.
- Bemisia tabaci /
- qucercetin /
- 2-tridecanone /
- cucurbitacin B /
- carboxylesterase /
- insecticide

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参考文献(24)

PRABHAKER A W,COUDRIET D L,MEGERDICK D E.Insecticide Resistance in the Sweetpotato Whitefly,Bemisia tabaci (Homoptera:Aleyrodidae)[J].Appl Entomol,1985,103:447-451.

PRABHAKER A W,COUDRIET D L,TOSCANO N C.Effect of Synergists on Organophosphate and Pemethrin Resistance in Sweetpoato Whitefly (Homoptear:Aleyrodidae)[J].Econ Entomol,1988,81:34-39.

CAHILL M,JARVIS W,GOMAN K,et al.Resolution of Baseline Response and Documentation of Resistance to Buprofezinin Bemisal tabaci (HomoPtera: Aleyrodidae)[J].Bull Entomol Res,1996,86:117-122.

ELBERT A,NAUEN R.Resistance of Bemisia tabaci (HomoPtera: Aleyrodidae) to Insecticides in Southern Spain Wiht Specia1 Refrence to Neonicotinoids[J].Pest Manag Sci,2000,56:60-64.

MOU Shao-fei(牟少飞),LIANG Pei(梁沛),GAO Xi-wu(高希武).槲皮素对B 型烟粉虱羧酸酯酶和谷胱甘肽S -转移酶活性的影响[J].Chinese Bull Entomolog(昆虫知识),2006,43(4): 491-495.

RILEY D G,TAN W.Host Plant Effects on Resistance to Bifenthrin in Silverleaf Whitefly (Homoptera: Aleyrodidae)[J].Econ Entomol,2003,96:1315-1321.

YU S J,BERRY R E,TERRIERE L C.Host Plant Stimulation of Detoxifying Enzymes in a Phytophagous Insect[J].Pestic Biochem Physiol,1979,12: 280-284.

BRATTSTEN L B.Potential role of Plant Allelochemicals in the Development of Insecticide Resistance[M]// BARBOSA P,LETOURNEAU D K.Novel Aspects of Instect-plant Interactions,New York: Wiley,1988 ∶ 313-348.

DONG Xiang-li(董向丽),GAO Xi-wu(高希武),ZHENG Bing-zong(郑炳宗).植物次生物质诱导作用对杀虫药剂毒力影响研究[J].Acta Entomologica Sinica(昆虫学报),1998,41(增):111-116.

GAO Xi-wu(高希武),DONG Xiang-li(董向丽).槲皮素对棉铃虫体内一些解毒酶系和靶标酶的诱导作用[J].Chin J Pestic Sci(农药学学报),1999, 1(3):56-60.

CHENF J,CHANG C Z,GAO X W.In vitro Inhibition of Glutathione S-transferases by Several Insecticides and Allelochemicals in Cotton Bollworm,Helicoverpa armigera Hübner[J].Entomol Sci,2007,42: 296-305.

LIANG P,CUI J Z,GAO X W,et al.Effects of Host Plants on Insecticide Susceptibility and Carboxylesterase Activity in Bemisia tabaci Biotype B and Greenhouse Whitefly,Trialeurodes vaporariorum[J].Pest Management Science,2007,4: 365-371.

BYRNE F J,GORMAN K J,CAHILL M,et al.The Role of B-type Esterases in Conferring Insecticide Resistance in the Tobacco Whitefly,Bemisia tabaci (Genn)[J].Pest Manag Sci,2000,56(10): 867-874.

BYRNE F J,DEVONSHIRE A L.Insensitive Acetylcholinesterase and Esterase Polymorphism in Susceptible and Resistant Populations of the Tobacco Whitefly,Bemisia tabaci (Genn) [J].Pestic Biochem Physiol,1993,45:34-42.

YAO Hｏｎɡ-ｗｅｉ(姚洪渭),YE Gｏｎɡ-ｙｉｎ(叶恭银),CHENG Jｉａ-ａｎ(程家安).寄主植物影响害虫药剂敏感性的研究进展[J].Acta Entomologica Sinica(昆虫学报),2002,45 (2):253-264.

DAVIDSON E W,FAY M L,BLACKMER J,et al.Improved Artificial Feeding System for Rearing the Whitefly Bemisia argentifolii (Homoptera: Aleyrodidae)[J].Florida Entomologist,2000,83(4):459-468.

VAN ASPERN.A Study of Housefly Esterase by Means of a Sensitive Colorimetric Method[J].Insect Physiol,1962,8: 401-406.

GAO Xi-wu(高希武),ZHENG Bing-zong(郑炳宗).几种农药对蚜虫羧酸酯酶的抑制和拟除虫菊酯的增效[J].J Beijing Agric Univ (北京农业大学学报),1991,17(4):89-94.

BRADFORD M M.A Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle of Protein-dye Binding[J].Anal Biochem,1976,7:248-254.

GAO Xi-wu(高希武).寄主植物对棉蚜羧酸酯酶活性的影响[J].Acta Entomologica Sinica(昆虫学报),1992,35 ∶ 267-272.

HEMINGWAY J,The Molecular Basis of Two Contrasting Metabolic Mechanisms of Insecticide Resistance[J].Insect Biochem Mol Biol,2000,30:1009-1015.

GAO Xi-wu(高希武),ZHAO Ying(赵颖),WANG Xu(王旭),et al.杀虫药剂和植物次生性物质对棉铃虫羧酸酯酶的诱导作用[J].Acta Entomologica Sinica(昆虫学报),1998,41(Sup.):5-11.

TANG Fang(汤方),LIANG Pei(梁沛),GAO Xi-wu(高希武).2-十三烷酮和槲皮素诱导棉铃虫谷胱甘肽S-转移酶组织特异性表达[J].Progress in Natural Science(自然科学进展),2005,(7):33-38.

LIU X N,LIANG P,GAO X W,et al.Induction of the Cytochrome P450 Activity by Plant Allelochemicals in the Cotton Bollworm,Helicoverpa armigera (Hübner )[J].Pestic Biochem Physiol,2006,84:127-134. (Ed.JIN S H)

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