采用纳米修饰双酶电极生物传感器检测有机膦与氨基甲酸酯类农药

干宁; 王峰; 杨欣; 李天华; 王军

采用纳米修饰双酶电极生物传感器检测有机膦与氨基甲酸酯类农药

干宁^1,,
王峰¹,
杨欣¹,
李天华¹,
王军^1,2

1.
新型功能材料及其制备科学国家重点实验室,宁波大学材料科学与化工学院,浙江宁波 315211
2.
南京农业大学理学院,南京 210011

基金项目:

浙江省自然科学基金(Y4080023);浙江省科技攻关项目(2006C31040);浙江省教育厅基金(20061697);宁波市自然科学基金(2008A610072;2008A610043);宁波大学王宽城基金资助项目. Key words: Fe3O4/Au nano particles; nano particles;acetylcholinesterase; choline oxidase; carbamate

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出版历程
- 收稿日期: 2008-04-08

A Nano Particles Modified Bienzyme Electrode Biosensor for theDetection of Carbamate and Organophosphorus Pesticides

1.
The State Key Laboratory of Novel Functional Materials and Preparation Science, Ningbo University,Ningbo 315211, Zhejiang Province,China
2.
College of Science, Nanjing Agricultural University, Nanjing 210011,China

摘要

摘要: 同时固定乙酸胆碱酯酶(AChE)及胆碱氧化酶(ChOx)于组装在丝网印刷电极表面的Fe3O4/Au纳米复合微粒上,构建了一类新颖的快速测定有机磷和氨基甲酸酯类农药的双酶传感器(AChE-ChOx/Fe3O4/Au SPCEs)。该双酶传感器电流响应在乙酸胆碱浓度为0.5 ~12.5 mmol/L之间呈良好线性关系(R2=0.998)。其对克百威(氨基甲酸酯类农药)和敌敌畏(有机磷类农药)的检测范围在0.05 ~1.00 μg/mL之间均呈良好线性关系(R2＝0.977),检测下限均可达0.01 μg/mL。应用于实际样品白菜的添加回收率在95％ ~110％之间,与传统的生化法相比具有良好相关性。此外该传感器成本低、制备容易、可抛弃,有望用于氨基甲酸酯和有机磷类农药的现场大规模筛测。
- Fe₃O₄/Au纳米微粒 /
- 纳米 /
- 乙酸胆碱酯酶 /
- 胆碱氧化酶 /
- 氨基甲酸酯
Abstract: A novel bienzyme electode biosensor for rapid determination of carbamate and orgnophosphorus pesticides was developed.The sensor was prepared by co-immobilizing acetylcholinesterase(AChE) and choline oxidase(ChOx) on a Fe3O4/Au nano particles surface modified screen printed electrode (AChE-ChOx/Fe3O4/Au SPCEs).The modified enzyme electrode showed linear current response to acetylcholine in the range of 0.5 ~12.5 mmol/L.It was utilized for carbofuran and dichlorovinyl dimethyl phosphate detection with a linearity range between 0.05 and 1.00 μg/mL (R2＝0.977).The detection limit may reach 0.01 μg/mL and their recovery exhibited a range of 95% ~110 % in chinese cabbage.The method using biosensor may be matched with the traditional biochemical method with low cost, desertable and easy preparation, thus would be valuable for rapid detection of carbamate and organophosphorus pesticides.

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参考文献(12)

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