鱼藤酮对小鼠脑神经递质多巴胺及其代谢产物的影响

林勇; 黄俊生; 本山植树

鱼藤酮对小鼠脑神经递质多巴胺及其代谢产物的影响

1.
中国热带农业科学院环境与植物保护研究所, 海南儋州 571737
2.
日本国立千叶大学园艺学部生态制御化学研究室,日本千叶县松户

基金项目:

"十一五"国家科技支撑计划项目(2007BAD48B03);中央级公益性科研院所基本科研业务费专项资助项目(2007hzs1J013).

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出版历程
- 收稿日期: 2008-01-04

Effects of Rotenone on the Brain Level of Dopamineand its Metabolites in Mice

1.
Institute of Environment and Plant Protection,Academy of Tropical Agriculture Sciences of China,Danzhou571737,Hainan Province,China
2.
Laboratory of Pesticide Toxicology,Faculty of Horticulture,Chiba University,648 Matsudo,Chiba 271-8510,Japan

摘要

摘要: 研究了注射鱼藤酮对C57BL/6N小鼠神经递质多巴胺的影响。给小鼠皮下注射鱼藤酮(10 mg/kg)两次,间隔2 h,在第2次注射8 h后分离脑组织样品,并应用高效液相色谱仪配电化学检测器,测定了小鼠脑内多巴胺(DA)及其主要代谢物3,4-二羟苯酰乙酸(DOPAC)和高香草酸(HVA)的水平。结果表明,第2次注射8 h后,鱼藤酮处理组的脑内DA、DOPAC和HVA水平分别比对照组高40.5%、544.4%和168.2%,差异显著(P<0.01)。实验显示,鱼藤酮对多巴胺能神经系统具有毒性作用。
- 鱼藤酮 /
- 神经递质 /
- 多巴胺 /
- 神经毒性 /
- 小鼠
Abstract: The effect of injection rotenone on the brain levels of dopamine (DA) and its metabolites (DOPAC and HVA) in C57BL/6N mice was studied.The mice were given two subcutaneous injections of rotenone (10 mg/kg) at 2 h interval between injections,and were killed at 8 hr after the second injection.The brain tissue was weighed and placed in a 5 mL tube containing ice-cooled perchl-oric acid (2 mL,0.4 mol/L),homogenized for 1 min at 20 000 r/min using an Ultra Turrax and centrifuged at 16 000 g for 12 min at 4℃.The supernatant was passed through a 0.2 μm filter and either directly analyzed or frozen at -70℃ until analysis.The DA and two of its metabolites were determined by HPLC with ECD.Results showed the percentage of increasing reached 40.5% (DA),544.4% (DOPAC),and 168.2% (HVA) as compare to the control (P<0.01) after the last injection.The rotenone has been suggested a potential nueurotoxicant that induced dopaminergic neurons to release dopamine and its metabolites in the mice.
- rotenone /
- neurotransmitter /
- dopamine /
- neurotoxicity /
- mice

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参考文献(18)

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