灰葡萄孢菌对啶菌噁唑的敏感性基线及抗药突变体的诱导与生物学性状
Study on Baseline Sensitivity of Botrytis cinerea to SYP-Z048 andInduction and Biological Characteristics of Resistant Mutants
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摘要: 从河北省不同地区未使用过啶菌噁唑的保护地中采集黄瓜或番茄灰霉病果、病叶,经单孢分离获得102株灰葡萄孢菌,采用菌丝生长速率法测定其对啶菌噁唑的敏感性,所得EC50值在0.046 0~0.199 1 μg/mL之间,平均为(0.118 2±0.036 3) μg/mL,其敏感性呈连续单峰曲线分布,可作为灰葡萄孢菌对啶菌噁唑的敏感性基线。采用紫外线诱导获得了7株抗药突变体,抗药突变体的菌落直径、菌丝干重、产孢量和致病性明显低于其亲本菌株,继代培养9代后抗药突变体的抗药性倍数下降。Abstract: A total of 102 single-spore isolates were collected from cucumber or tomato infected by Botrytis cinerea without exposure to SYP-Z048 in protected fields in different regions of Hebei Province.The sensitivity of the isolates was determined by mycelial growth rate inhibition tests.The EC50 value distributed as a unimodal curve,which ranging from 0.046 0 μg/mL to 0.199 1 μg/mL,with a mean value of (0.118 2±0.036 3) μg/mL.The data could be used as baseline sensitivity of B.cinerea to SYP-Z048.Seven SYP-Z048-resistant mutants were obtained through ultraviolet radiation.The colonies diameter,mycelial weight,sporulation and pathogenicity ability of resistant mutants were significantly lower than that of parental isolates.The resistance rate of resistant mutants receded after nine incubations.
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Key words:
- Botrytis cinerea /
- SYP-Z048 /
- baseline sensitivity /
- resistant mutants /
- biological characteristics
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