分子靶标烟草花叶病毒外壳蛋白的结构生物学研究进展

李向阳; 陈卓; 杨松; 宋宝安

分子靶标烟草花叶病毒外壳蛋白的结构生物学研究进展

1.
贵州大学精细化工研究开发中心,绿色农药与农业生物工程国家重点实验室培育基地/教育部重点实验室,贵阳 550025

基金项目:

国家重点基础研究发展计划(973计划)项目(2010CB126105);国家自然科学基金(20872021)资助项目.

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出版历程
- 收稿日期: 2010-05-20
- 修回日期: 2010-08-07

Research progress of structure biology of tobacco mosaic virus coat protein as molecular targets

1.
Research and Development Center for Fine Chemicals,State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering,Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Guizhou University,Guiyang 550025,China

摘要

摘要: 病毒外壳蛋白(CP)是烟草花叶病毒(TMV)基因结构的重要组成部分,其主要功能除保护核酸物质不被降解外,还与TMV的远距离移动及寄主症状有关。目前研究较多的是CP基因介导的抗性、药剂干扰CP的聚合等,而有关靶标CP与药剂的作用机理尚未有系统全面的报道。从结构生物学的角度出发,详细介绍了TMV CP在电镜及单晶衍射技术下的三维结构;比较了国内已报道的CP的基因序列;综述了CP在基因工程领域的应用及其受药剂分子的干扰作用等方面的研究进展。以毒氟磷药剂为例,分析了其可能作用于TMV CP的分子机理,预测了毒氟磷小分子与TMV CP大分子可能的作用位点。有助于进一步研究和发现新型抗TMV药剂。
- 烟草花叶病毒 /
- 外壳蛋白 /
- 分子靶标 /
- 结构生物学 /
- 综述
Abstract: Coat protein(CP) is an important part of the genomic structure of the tobacco mosaic virus(TMV),its main function not only includes the protection of DNA from degradation,but also has a close relationship with the TMV long-distance movement and host symptoms.Presently,the study of TMV CP mainly focus on CP-mediated resistance,interference the polymerization of CP by agent and so on,while the mechanism of agent action with target CP has not been comprehensively reported.From the perspective of structural biology,the three-dimensional structure of TMV analyzed by electron microscopy and diffraction techniques using TMV CP as a molecular target was detailed;the CP gene sequences which had been reported in China was compared;the progress of applying TMV CP in genetic engineering and interfering of agent molecules with CP were reviewed.Moreover,the possible mechanism of agent(dufulin) on TMV CP was analyzed and the active sites between dufulin molecule and macromolecules of TMV CP were forecasted,which would facilitate further research and developing novel anti-TMV agents of tobacco.
- tobacco mosaic virus(TMV) /
- coat protein(CP) /
- molecular target /
- structural biology /
- research progress

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