三种有机磷农药胚胎期暴露对新生鼠脑组织结构的影响

陈伟峰; 陈晓萍; 陈文珍

三种有机磷农药胚胎期暴露对新生鼠脑组织结构的影响

1.
浙江工业大学生物与环境工程学院,杭州 310014

基金项目:

国家自然科学基金资助项目(30972446).

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出版历程
- 收稿日期: 2011-01-25
- 修回日期: 2011-04-22

Effects of prenatal exposure of three organophosphorus pesticides on neonatal brain structure

1.
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China

摘要

摘要: 探讨了3种常用的有机磷农药胚胎期暴露对新生鼠脑组织结构的影响。在母鼠妊娠期7.5～11.5 d时,连续5 d每天分别经皮下注射2 mg/kg bw的二嗪磷(diazinon)、2 mg/kg bw的毒死蜱(chlorpyrifos)及50 mg/kg bw乙酰甲胺磷(acephate),显微镜下观察并计数大脑皮层S1区胶质细胞和神经元数量及比率,以及海马CA1、CA3区锥体细胞的数量及体积密度。结果表明,二嗪磷与毒死蜱处理分别使得大脑皮层S1区胶质细胞数减少了14.29%和21.43%;毒死蜱处理导致胶质细胞与神经元比率下降了24.19%;二嗪磷与毒死蜱处理后海马CA1区锥体细胞数量分别下降了9.30％与20.93％,毒死蜱处理后锥体细胞体积密度下降了27.05％;二嗪磷与毒死蜱处理后海马CA3区锥体细胞数量分别下降了22.22％和19.44％,锥体细胞体积密度分别下降了23.54％和18.98％;而乙酰甲胺磷对新生鼠大脑皮层与海马细胞均无明显影响。
- 有机磷农药 /
- 胚胎期 /
- 鼠 /
- 脑组织 /
- 皮层S1区 /
- 海马区
Abstract: Effects of prenatal exposure of three organophosphorus pesticides on neonatal brain structure in mice were investigated.2 mg/kg bw of diazinon,2 mg/kg bw of chlorpyrifos and 50 mg/kg bw of acephate were injected subcutaneously daily during gestation days 7.5-11.5 of ICR mice.Quantitative morphology in hippocampus and somatosensory cortex were measured.The results showed that diazinon and chlorpyrifos caused glia number in somatosensory cortex reduced by 14.29% and 21.43%,respectively,and chlorpyrifos caused the ratio of glia and neuron reduced by 24.19%.The number of pyramidal cell in hippocampus CA1 decreased by 9.30％ and 20.93％ after treatment of diazinon and chlorpyrifos,respectively,and the volumn density of pyramidal cell decreased by 27.05％ after treatment of chlorpyrifos.Meanwhile,the number of pyramidal cell number in hippocampus CA3 decreased by 22.22％ and 19.44％,and the volumn density of pyramidal cell decreased by 23.54％ and 18.98％ after treatment of diazinon and chlorpyrifos,respectively.Acephate had no significant effect on somatosensory cortex and hippocampus.
- organophosphorus pesticide /
- embryonic period /
- mouse /
- brain structure /
- somatosensory cortex /
- hippocampus

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参考文献(16)

BERMAN T,HOCHNER-CELNIKIER D,BARR D B,et al.Pesticide exposure among pregnant women in Jerusalem,Israel:results of a pilot study[J].Environ Int,2011,37(1):198-203.

KAWAHARA J,YOSHINAGA J,YANAGISAWA Y,et al.Dietary exposure to organophosphorus pesticides for young children in Tokyo and neighboring area[J].Sci Total Environ,2007,378(3):263-268.

SLOTKIN T A,SEIDLER F J.Prenatal chlorpyrifos exposure elicits presynaptic serotonergic and dopaminergic hyperactivity at adolescence:critical periods for regional and sex-selective effects[J].Reprod Toxicol,2007,23(3):421-427.

ROY T S,SEIDLER F J,SLOTKIN T A.Morphologic effects of subtoxic neonatal chlorpyrifos exposure in developing rat brain:regionally selective alterations in neurons and glia[J].Brain Res Dev Brain Res,2004,148(2):197-206.

US Environmental Protection Agency Office of Pesticide Programs.0238:Interim reregistration eligibility decision for diazinon[S].Washington D.C:Office of Prevention,Pesticides and Toxic Substances,2002.

US Environmental Protection Agency Office of Pesticide Programs.0100:Interim reregistration eligibility decision for chlorpyrifos[S].Washington D.C:Office of Prevention,Pesticides and Toxic Substances,2002.

US Environmental Protection Agency Office of Pesticide Programs.0042:Interim reregistration eligibility decision for acephate[S].Washington D.C:Office of Prevention,Pesticides and Toxic Substances,2001.

ZHANG J Y,MILLER M I,PLACHEZ C,et al.Mapping postnatal mouse brain development with diffusion tensor microimaging[J].NeuroImage,2005,26(4):1042-1051.

BAO Xin-min(包新民),SHU Si-yun(舒斯云).The Stereotaxic Atlas of The Rat Brain(大鼠脑立体定位图谱)[M].Beijing(北京):People's Medical Publishing House(人民卫生出版社),1991:46-47.

WANG Xiao-dong(王晓冬),TANG Le-min(汤乐民).Biological Light Microscope Specimen Technology(生物光镜标本技术)[M].Beijing(北京):Science Press(科学出版社),2007:257-265.

GU Qing(辜清),YAO Li-xi(姚立希),WU di(吴娣),et al.脑缺血再灌流后大鼠海马锥体细胞和星形胶质细胞的体视学[J].J Nanchang Univ(南昌大学学报),2009,33:2-6.

ROY T S,SHARMA V,SEIDLER F J,et al.Quantitative morphological assessment reveals neuronal and glial deficits in hippocampus after a brief subtoxic exposure to chlorpyrifos in neonatal rats[J].Brain Res Dev Brain Res,2005,155(1):71-80.

TIMOFEEVA O A,ROEGGE C S,SEIDLER F J,et al.Persistent cognitive alterations in rats after early postnatal exposure to low doses of the organophosphate pesticide,diazinon[J].Neurotoxicol Teratol,2008,30(1):38-45.

RICCERI L,VENEROSI A,CAPONE F,et al.Developmental neurotoxicity of organophosphorous pesticides:fetal and neonatal exposure to chlorpyrifos alters sex-specific behaviors at adulthood in mice[J].Toxicol Sci,2006,93(1):105-113.

VENEROSI A,CALAMANDREI G,RICCERI L.A social recognition test for female mice reveals behavioral effects of developmental chlorpyrifos exposure[J].Neurotoxicol Teratol,2006,28(4):466-471.

LEVIN E D,ADDY N,BARUAH A,et al.Prenatal chlorpyrifos exposure in rats causes persistent behavioral alterations[J].Neurotoxicol Teratol,2002,24(6):733-741.

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