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水稻白叶枯病菌室内链霉素抗性菌株的诱导及其抗性分子机制和风险评价

徐颖 祝晓芬 周明国

徐颖, 祝晓芬, 周明国. 水稻白叶枯病菌室内链霉素抗性菌株的诱导及其抗性分子机制和风险评价[J]. 农药学学报, 2013, 15(1): 43-51.
引用本文: 徐颖, 祝晓芬, 周明国. 水稻白叶枯病菌室内链霉素抗性菌株的诱导及其抗性分子机制和风险评价[J]. 农药学学报, 2013, 15(1): 43-51.
XU Ying, ZHU Xiaofen, ZHOU Mingguo. Molecular mechanism and resistance risk assessment of laboratory-induced streptomycin-resistant mutants of Xanthomonas oryzae pv.oryzae[J]. Chinese Journal of Pesticide Science, 2013, 15(1): 43-51.
Citation: XU Ying, ZHU Xiaofen, ZHOU Mingguo. Molecular mechanism and resistance risk assessment of laboratory-induced streptomycin-resistant mutants of Xanthomonas oryzae pv.oryzae[J]. Chinese Journal of Pesticide Science, 2013, 15(1): 43-51.

水稻白叶枯病菌室内链霉素抗性菌株的诱导及其抗性分子机制和风险评价

基金项目: 

国家高技术研究发展计划(863计划)(2012AA101502);国家自然科学基金(31100100);公益性行业(农业)科研专项(201303023).

Molecular mechanism and resistance risk assessment of laboratory-induced streptomycin-resistant mutants of Xanthomonas oryzae pv.oryzae

  • 摘要: 通过紫外光照射诱导获得了6株水稻白叶枯病菌Xanthomonas oryzae pv.oryzae抗链霉素突变体。测得链霉素对水稻白叶枯病菌敏感菌株ZJ173及其抗性突变体的最低抑制浓度(MIC)分别为0.10和600 μg/mL;对敏感菌株的有效抑制中浓度(EC50)为0.03 μg/mL,对抗性菌株的平均EC50值为11.64 μg/mL,平均抗性倍数为388。通过PCR扩增了敏感菌株ZJ173及5株抗性菌株的rpsL基因(编码S12核糖体蛋白)和rrs基因(编码16S rRNA),并检测了strA基因是否存在。序列分析表明,5株被测抗性菌株的rpsL基因均发生了突变,其中4株在氨基酸43位、1株在88位,均由赖氨酸突变为精氨酸,而rrs基因未发生突变,strA基因未被检测到。表明实验室诱导获得的水稻白叶枯病菌抗性菌株对链霉素的抗药性是由rpsL基因突变引起的。抗性风险研究表明,抗性突变体的抗药性在无药剂压力下可稳定保持,其致病性、生长速率与敏感菌株相比无明显差异,竞争性低于或略低于敏感菌株,抗性自发突变率较高,且抗性突变为单一位点突变,病害循环为多循环,因此由rpsL基因突变引起的水稻白叶枯病菌对链霉素的抗性风险较高。
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  • 收稿日期:  2012-06-24
  • 修回日期:  2012-10-06

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