Cylindrocarpon olidum W1菌株代谢物中壳二孢氯素及其类似物的分离及抑菌活性

魏少鹏; 姬志勤

doi:10.16801/j.issn.1008-7303.2017.0060

Cylindrocarpon olidum W1菌株代谢物中壳二孢氯素及其类似物的分离及抑菌活性

doi: 10.16801/j.issn.1008-7303.2017.0060

魏少鹏^1,2,
姬志勤^1,2,

1.
西北农林科技大学植物保护学院, 陕西杨凌 712100
2.
西北农林科技大学陕西省植物源农药研究与开发重点实验室, 陕西杨凌 712100

基金项目:

国家重点研发计划（2017YFD0200506）;国家自然科学基金资助项目（31371973，31301700）;陕西省科技攻关项目（2016NY-139）

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出版历程
- 收稿日期: 2017-06-09
- 刊出日期: 2017-08-09

Isolation and antimicrobial activities of ascochlorin and its analogues from the metabolites of Cylindrocarpon olidum W1

WEI Shaopeng^1,2,
JI Zhiqin^{1,2
,}

1.
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
2.
Shaanxi Province Key Laboratory Research & Development on Botanical Pesticides, Northwest A&F University, Yangling 712100, Shaanxi Province, China

摘要

摘要: 采用硅胶柱层析及高效液相色谱等技术从冬青卫矛内生真菌Cylindrocarpon olidum W1次生代谢物中分离鉴定出8个抑菌活性化合物W1-1~W1-8。经核磁共振氢谱和碳谱、高分辨质谱等技术，并结合相关文献确认其为壳二孢氯素及其类似物，分别为Ilicicolin A（W1-1）、5-Chlorocolletorin B（W1-2）、Ilicicolin B（W1-3）、Deacetylchloronectrin（W1-4）、Ilicicolin C（W1-5）、壳二孢氯素（W1-6）、Cylindrol A4（W1-7）和Cylindrol B（W1-8）。抑菌活性测定结果表明：壳二孢氯素及其类似物对烟草青枯病菌有较强的抑制作用，上述化合物的最小抑制浓度分别为6.25、3.13、>；100、3.13、6.25、12.5、25和25 μg/mL；化合物W1-2、W1-4和W1-5对番茄灰霉病菌和油菜菌核病菌菌丝生长亦有较强的抑制作用，其中对番茄灰霉病菌的抑制中浓度（IC50）分别为36.45、21.60和26.69 μg/mL，对油菜菌核病菌的抑制中浓度分别为20.21、16.79和12.11 μg/mL。
- Cylindrocarpon olidum W1 /
- 壳二孢氯素 /
- 分离鉴定 /
- 抑菌活性
Abstract: Eight compounds W1-1–W1-8 were isolated from the secondary metabolites of Cylindrocarpon olidum W1, which is an endophytic fungus from Euonymus japonica, by silica gel column chromatography and pre-HPLC techniques. Based on the 1H NMR and 13C NMR and HRMS-MS data, as well as the published literatures, these chemicals were identified as Ilicicolin A (W1-1), 5-Chlorocolletorin B (W1-2), Ilicicolin B (W1-3), Deacetylchloronectrin (W1-4), Ilicicolin C (W1-5), Ascochlorin (W1-6), Cylindrol A4 (W1-7) and Cylindrol B (W1-8). The obtained Ascochlorin and its analogues showed strong antibacterial activities against Ralstonia solanacearum, with minimum inhibitory concentrations (MICs) of 6.25, 3.13, 100, 3.13, 6.25, 12.5, 25 and 25 μg/mL, respectively. Compounds W1-2, W1-4 and W1-5 could also effectively inhibit the mycelial growth of plant pathogenic fungi Botrytis cinerea and Sclerotinia sclerotiorum. Their medium inhibitory concentrations (IC50) against B. cinerea were 36.45, 21.60 and 26.69 μg/mL, respectively. Their IC50 values against S. sclerotiorum were 20.21, 16.79 and 12.11 μg/mL, respectively.
- Cylindrocarpon olidum W1 /
- ascochlorin /
- isolation and identification /
- antimicrobial activity

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参考文献(17)

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