香蕉中2,4-滴残留的快速检测及其消解动态

林涛; 黎其万; 刘宏程; 邵金良; 赵金化; 范泽剑

doi:10.16801/j.issn.1008-7303.2017.0068

香蕉中2,4-滴残留的快速检测及其消解动态

doi: 10.16801/j.issn.1008-7303.2017.0068

林涛^1,2,
黎其万^1,2,
刘宏程^1,2,,
邵金良¹,
赵金化³,
范泽剑³

1.
云南省农业科学院质量标准与检测技术研究所, 昆明 650223
2.
农业部农产品质量安全风险评估实验室(昆明), 昆明 650223
3.
云南省大理市农产品质量安全检测站, 云南大理 671000

基金项目:

云南省社会发展科技计划（2012CA005）;云南省科技创新平台建设计划（2014DA001）

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出版历程
- 收稿日期: 2017-01-22
- 刊出日期: 2017-08-09

Rapid determination and dissipation dynamics of 2,4-D in Musa paradisiaca

1.
Institute of Agriculture Quality Standards & Testing Technique, Yunnan Academy of Agricultural Science, Kunming 650223, China
2.
Laboratory of Quality & Safety Risk Assessment for Agro-Products (Kunming), Ministry of Agriculture, Kunming 650223, China
3.
Quality and Safety Inspection Station of Agricultural Products in Dali City, Dali 671000, Yunnan Province, China

摘要

摘要: 利用超高效液相色谱-串联质谱建立了香蕉中2，4-滴残留的快速测定方法，研究了2，4-滴在香蕉中的残留消解动态和最终残留量。香蕉样品经乙腈提取，氯化钠盐析分层后，采用负离子电离，多反应监测模式（MRM）测定。结果表明：在1.0~10 μg/L范围内，2，4-滴的质量浓度与对应的峰面积间呈良好线性关系，检出限为0.001 5 mg/kg，定量限为0.005 mg/kg。在0.005、0.05和0.5 mg/kg 3个添加水平下，2，4-滴的平均回收率为93%~99%，相对标准偏差为2.5%~3.0%，可满足香蕉中2，4-滴残留检测的要求。田间试验结果表明，2，4-滴在香蕉中的消解半衰期为5.4 d，属易降解农药。最终残留试验结果表明，以有效成分分别为23、35和53 mg/kg的剂量施药，于60 d后采收时，香蕉中2，4-滴的残留量均<；LOQ。
- 香蕉 /
- 2,4-滴 /
- 残留 /
- 快速检测 /
- 消解动态 /
- 超高效液相色谱-串联质谱
Abstract: An ultra high performance liquid chromatography-tandem mass spectrometry method was developed to study the dissipation dynamics and final residues of 2,4-D in Musa paradisiaca. The M. paradisiaca samples were extracted by acetonitrile, salted out and stratified by sodium chloride. Using negative ion ionization and multiple reaction monitoring, the results showed that there was a good linear relationship between the concentrations of 2,4-D and the corresponding peak area in the range of 1.0 μg/L to 10 μg/L. The limits of detection and quantification of the method were 0.001 5 mg/kg and 0.005 mg/kg, respectively. The average recoveries was in the range of 93% to 99% with the relative standard deviations from 2.5% to 3.0% at three spiked levels of 0.005, 0.05 and 0.5 mg/kg. The method is suitable for the 2,4-D residues detection in M. paradisiaca. The results of field experiments show that the half-life of 2,4-D was 5.4 days, indicating that 2,4-D is a non-persistent and easily degradable pesticide. The final residue test results showed that 2,4-D was LOQ in the M. paradisiaca 60 days after harvest, at dosages of of 23, 35 and 53 mg/kg (effective component concentration), respectively.
- Musa paradisiaca /
- 2,4-D /
- residue /
- rapid determination /
- dissipation dynamics /
- ultra high performance liquid chromatography-tandem mass spectrometry

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参考文献(18)

PAZMIÑO D M, ROMERO-PUERTAS M C, SANDALIO L M. Insights into the toxicity mechanism of and cell response to the herbicide 2,4-D in plants[J]. Plant Signal Behav, 2012, 7(3):425-427.

VAN OVERBEEK J. Plant hormones and regulators[J]. Science, 1966, 152(3723):721-731.

HANSEN W H, QUAIFE M L, HABERMANN R T, et al. Chronic toxicity of 2,4-dichlorophenoxyacetic acid in rats and dogs[J]. Toxicol Appl Pharmacol, 1971, 20(1):122-129.

GARABRANT D H, PHILBERT M A. Review of 2,4-dichlorophenoxyacetic acid (2,4-D) epidemiology and toxicology[J]. Crit Rev Toxicol, 2002, 32(4):233-257.

PURCELL M, NEAULT J F, MALONGA H, et al. Interactions of atrazine and 2,4-D with human serum albumin studied by gel and capillary electrophoresis, and FTIR spectroscopy[J]. Biochim Biophys Acta, 2001, 1548(1):129-138.

PAVLICA M, PAPEŠ D, NAGY B. 2,4-Dichlorophenoxyacetic acid causes chromatin and chromosome abnormalities in plant cells and mutation in cultured mammalian cells[J]. Mutat Res Lett, 1991, 263(2):77-81.

食品中农药最大残留限量:GB 2763-2016[S]. 北京:中国标准出版社, 2017.Maximum residue limits for pesticides in food:GB 2763-2016[S]. Beijing:Standards Press of China, 2017.

常宇文, 吴晓宗, 李伟, 等. 凝胶渗透色谱-气相色谱法测定豆芽中2,4-二氯苯氧乙酸残留量[J]. 食品工业科技, 2008, 28(12):203-205.CHANG Y W, WU X Z, LI W, et al. Determination of residues of 2,4-dichlorophenoxyacetic in bean sprout by gas chromatography and gel permeation chromatography[J]. Sci Technol Food Ind, 2008, 28(12):203-205.

BEESON M D, DRISKELL W J, BARR D B. Isotope dilution high-performance liquid chromatography/tandem mass spectrometry method for quantifying urinary metabolites of atrazine, malathion, and 2,4-dichlorophenoxyacetic acid[J]. Anal Chem, 1999, 71(16):3526-3530.

DZANTIEV B B, ZHERDEV A V, YULAEV M F, et al. Electrochemical immunosensors for determination of the pesticides 2,4-dichlorophenoxyacetic and 2,4, 5-tricholorophenoxyacetic acids[J]. Biosens Bioelectron, 1996, 11(1-2):179-185.

余若祯, 何苗, 施汉昌, 等. 间接竞争ELISA方法测定水中2,4-D的研究[J]. 生态毒理学报, 2006, 1(1):88-92.YU R Z, HE M, SHI H C, et al. Development of indirect ELISA for the determination of 2,4-D in water[J]. Asian J Ecotoxicol, 2006, 1(1):88-92.

HAUPT K, DZGOEV A, MOSBACH K. Assay system for the herbicide 2,4-dichlorophenoxyacetic acid using a molecularly imprinted polymer as an artificial recognition element[J]. Anal Chem, 1998, 70(3):628-631.

林珍珍, 张水锋, 曹慧, 等. 超高效液相色谱-串联质谱法测定肉制品中非法添加的14种酸性染料[J]. 中国食品学报, 2013, 13(5):167-172.LIN Z Z, ZHANG S F, CAO H, et al. UPLC-MS/MS determination of fourteen acid dyes Illegally added in meat products[J]. J Chin Inst Food Sci Technol, 2013, 13(5):167-172.

马晓燕, 朱顺达, 肖海龙, 等. 液相色谱-串联质谱法测定食品中喹啉黄、酸性绿S和专利蓝V[J]. 中国食品学报, 2012, 12(4):182-186.MA X Y, ZHU S D, XIAO H L, et al. Determination of quinoline yellow, green S and patent blue V in foods by performance liquid chromatography tandem mass spectrometry[J]. J Chin Inst Food Sci Technol, 2012, 12(4):182-186.

马婧玮, 李通, 周玲, 等. 烯效唑在棉花及土壤中的残留及消解动态[J]. 农药学学报, 2017, 19(3):374-380.MA J W, LI T, ZHOU L, et al. Residue and dissipation of uniconazole in Gossypium spp. and soil[J]. Chin J Pest Sci, 2017, 19(3):374-380.

林涛, 邵金良, 刘兴勇, 等. QuEChERS-超高效液相色谱-串联质谱法测定蔬菜中41种农药残留[J]. 色谱, 2015, 33(3):235-241.LIN T, SHAO J L, LIU X Y, et al. Determination of 41 pesticide residues in vegetables by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J]. Chin J Chromatogr, 2014, 33(3):235-241.

农药残留试验准则:NY/T 788-2004[S]. 北京:中国农业出版社, 2004.Guideline on pesticide residue trials:NY/T 788-2004[S]. Beijing:China Agriculture Press, 2004.

韦婕, 邓婕, 黄慧俐, 等. 高效液相色谱检测呋虫胺在稻田水和土壤中的残留及消解动态[J]. 农药学学报, 2015, 17(2):195-200.WEI J, DENG J, HUANG H L, et al. Residue and decline study of dinotefuran in paddy water and soil by high performance liquid chromatographic[J]. Chin J Pest Sci, 2015, 17(2):195-200.

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