Resistance of Eleusine indica to acetyl-CoA carboxylase-inhibiting herbicides in direct-seeded rice and its molecular mechanism
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摘要: 为明确直播稻田牛筋草对乙酰辅酶A羧化酶 (ACCase) 类除草剂的抗药性水平及其抗性产生的分子机制，采用整株生物测定法测定了牛筋草对6种ACCase类除草剂的抗性水平，并分别对抗性种群和敏感种群的ACCase基因部分片段进行了扩增和测序。结果表明：疑似抗性种群SJ-1对唑酰草胺、氰氟草酯、精唑禾草灵、高效氟吡甲禾灵和烯禾啶产生了高水平抗性，其抗性倍数分别为56.6、62.5、128、52.0和16.3；对烯草酮产生了低水平抗性，相对抗性倍数为4.86。将抗性种群和敏感种群的ACCase基因片段序列进行比对分析发现，SJ-1种群ACCase基因2078位氨基酸由天冬氨酸 (GAT) 突变为甘氨酸 (GGT)，该位点氨基酸突变可能是其对ACCase类除草剂产生抗药性的主要原因之一。
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关键词:
- 牛筋草 /
- 乙酰辅酶A羧化酶类除草剂 /
- 抗性 /
- 基因突变 /
- 分子水平
Abstract: The objectives of this study were to illuminate the sensitivity of Eleusine indica population in rice field of direct seeding to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides and reveal its molecular resistance mechanism. Whole-plant bioassay showed that the population SJ-1 was highly resistant to metamifop, cyhalofop-butyl, fenoxaprop-p-ethyl, haloxyfop-R-methyl and sethoxydim, with resistance indexes of 56.6, 62.5, 128, 52.0 and 16.3, respectively. And it was slightly resistant to clethodim, with a resistance index of 4.86. By comparing the partial ACCase sequences derived from the resistant and susceptible populations, the SJ-1 population was found to contain one amino acid substitution of aspartate (GAT) for glycine (GGT) at the codon position 2078. This mutation in the ACCase gene might be responsible for the resistance to ACCase-inhibiting herbicides in the resistant population of E. indica.-
Key words:
- Eleusine indica /
- ACCase-inhibiting herbicide /
- resistance /
- gene mutation /
- molecular mechanism
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图 1 牛筋草抗性和敏感种群对ACCase类除草剂的生长剂量-反应曲线
Figure 1. Dose–response curves for resistant and susceptible E. indica populations to ACCase-inhibiting herbicides
图 2 牛筋草抗性种群ACCase 2078位氨基酸突变的序列分析
Figure 2. Sequence alignment and derived amino acids at position 2078 of the ACCase gene from E. indica
表 1 整株生物测定中所用除草剂的剂量
Table 1. Herbicides treatments applied for whole-plant bioassay
除草剂
Herbicide剂量 (有效成分) Dose, a.i./(g/hm2) 抗性种群 SJ-1
Resistant population SJ-1敏感种群 FX-1
Susceptible population FX-1唑酰草胺 metamifop 15.0，30.0，120，480，1 920，3 840 1.88，3.75，7.50，30.0，120，240 氰氟草酯 cyhalofop-butyl 13.1，26.2，105，420，1 680，3 360 1.64，3.28，6.56，26.2，105，210 精唑禾草灵fenoxaprop-p-ethyl 12.9，51.8，207，828，1 656，3 312 1.62，3.23，12.9，51.8，104，414 高效氟吡甲禾灵haloxyfop-R-methyl 8.10，16.2，64.8，259，1 036，2 073 0.51，1.01，2.02，4.05，16.2，64.8 烯禾啶 sethoxydim 11.7，46.9，188，750，3 000，6 000 5.86，11.7，46.9，188，750，3 000 烯草酮 clethodim 2.25，4.50，18.0，72.0，288，1 152 2.25，4.50，18.0，72.0，288，1 152 注：粗体数字表示田间推荐剂量。Note: The number in bold stand for the recommended filed dose. 
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表 2 牛筋草抗性和敏感种群对ACCase类除草剂的敏感性
Table 2. Sensitivity of the resistant and susceptible E. indica populations to ACCase-inhibiting herbicides
除草剂
Herbicide抑制中剂量 (有效成分) GR50, a.i./(g/hm2) 抗性倍数
Resistance index抗性种群 SJ-1
Resistant population SJ-1敏感种群 FX-1
Susceptible population FX-1唑酰草胺 metamifop 269 ± 27 4.75 ± 1.34 56.6 氰氟草酯 cyhalofop-butyl 333 ± 28 5.33 ± 0.56 62.5 精唑禾草灵 fenoxaprop-p-ethyl 884 ± 49 6.91 ± 0.96 128 高效氟吡甲禾灵 haloxyfop-R-methyl 78.6 ± 13.1 1.51 ± 0.13 52.0 烯禾啶 sethoxydim 453 ± 57 27.8 ± 1.3 16.3 烯草酮 clethodim 70.5 ± 5.8 14.5 ± 1.4 4.86 注：表中数据为平均值 ± 标准差。Note: Data in the table are expressed as mean ± standard deviation.
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