多菌灵等4种杀菌剂对烟草灰霉病菌的室内生物活性

周浩; 李丽翠; 樊杰; 余知和; 汪汉成; 陈兴江; 马骏; 刘锦华

doi:10.16801/j.issn.1008-7303.2019.0033

多菌灵等4种杀菌剂对烟草灰霉病菌的室内生物活性

doi: 10.16801/j.issn.1008-7303.2019.0033

周浩^{1, 2,},
李丽翠^{1, 2,},
樊杰^{1, 2},
余知和¹,
汪汉成^2, ,,
陈兴江²,
马骏³,
刘锦华^3, ,

1.
长江大学生命科学学院，湖北荆州 434025
2.
贵州省烟草科学研究院，贵阳 550081
3.
贵州省烟草公司黔西南州公司，贵州兴义 562400

基金项目: 国家自然科学基金 (31501679)；贵州省科技厅优秀青年人才培养计划 (黔科合平台人才[2017]5619)；贵州省科技支撑计划 (黔科合支撑[2018]2356)；中国烟草总公司贵州省公司科技项目 (201711，201714)

详细信息

作者简介:
^周浩，男，硕士研究生，E-mail：919355397@qq.com

^李丽翠，并列第一作者，女，硕士研究生，E-mail：2392693846@qq.com

通讯作者:
汪汉成，通信作者 (Author for correspondence)，男，博士，研究员，主要从事烟草植物保护和微生物学研究，E-mail：xiaobaiyang126@hotmail.com

刘锦华，共同通信作者 (Co-author for correspondence)，男，农艺师，主要从事烟草生产及行业管理研究，E-mail：majun161@163.com

中图分类号: S482.2; TQ450.21
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出版历程
- 收稿日期: 2018-11-08
- 录用日期: 2019-01-17
- 刊出日期: 2019-04-01

In vitro bioactivities of four fungicides including carbendazim against Botrytis cinerea in tobacco

ZHOU Hao^{1, 2
,},
LI Licui^{1, 2
,},
FAN Jie^{1, 2},
YU Zhihe¹,
WANG Hancheng^{2
, ,},
CHEN Xingjiang²,
MA Jun³,
LIU Jinhua^{3
, ,}

1.
College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei Province, China
2.
Guizhou Academy of Tobacco Sciences, Guiyang 550081, China
3.
Guizhou Provincial Qianxinan Tobacco Company, Xingyi 562400, Guizhou Province, China

摘要

摘要: 采用菌丝生长速率法和孢子萌发法，分别测定了烟草灰霉病菌对多菌灵、嘧霉胺、异菌脲和丙环唑的敏感性，同时通过离体叶片法评估了这4种杀菌剂对烟草灰霉病的保护和治疗作用。结果表明：4种杀菌剂对烟草灰霉病菌的菌丝生长和孢子萌发均表现出了不同程度的抑制活性，并对灰霉病同时具有保护和治疗作用。其中多菌灵对菌丝生长的抑制活性最强，EC₅₀平均值为0.06 mg/L，其次为丙环唑、嘧霉胺和异菌脲，EC₅₀平均值分别为0.36、0.53和0.60 mg/L；异菌脲和丙环唑对烟草灰霉病菌孢子萌发的抑制活性较强，EC₅₀平均值分别为2.05和2.21 mg/L，其次为嘧霉胺和多菌灵，EC₅₀平均值分别为10.56和131.23 mg/L。异菌脲和多菌灵对灰霉病的保护作用和治疗作用均最强，当药剂质量浓度为200 mg/L时，其对离体叶片的保护和治疗作用防效分别为100%、100%和98.3%、91.8%。研究结果可为烟草灰霉病的科学防治提供依据。
- 烟草灰霉病 /
- 灰葡萄孢 /
- 多菌灵 /
- 嘧霉胺 /
- 异菌脲 /
- 丙环唑 /
- 敏感性 /
- 防治效果
Abstract: The in vitro bioactivities of four fungicides (carbendazim, iprodione, pyrimethanil, propiconazole) against Botrytis cinerea from tobacco were investigated using mycelial growth rate method and spore germination method. Their protective and curative activities against grey mould on detached leaves were investigated. The results showed that the four fungicides had different inhibitory activities. The fungicide with the highest toxic potential against the mycelial growth of B. cinerea was carbendazim with average EC₅₀ value of 0.06 mg/L, followed by propiconazole, pyrimethanil and iprodione, with average EC₅₀ values of 0.36, 0.53 and 0.60 mg/L, respectively. The EC₅₀ values of fungicides against spore germination were as followings: iprodione (2.05 mg/L), propiconazole (2.21 mg/L), pyrimethanil (10.56 mg/L) and carbendazim (131.23 mg/L). The most effective fungicide with the best protective and curative activities was iprodione and carbendazim. At the concentration of 200 mg/L, protective control efficacies were both 100% and the curative efficacies were 98.3% and 91.8%, respectively. This study provided scientific basis for guiding the control of tobacco grey mould.
- tobacco grey mould /
- Botrytis cinerea /
- carbendazim /
- pyrimethanil /
- iprodione /
- propiconazole /
- sensitivity /
- control efficacy

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表 1 供试4种杀菌剂对烟草灰霉病菌菌丝生长的抑制作用

Table 1. Inhibitory effects of four fungicides against the mycelium growth of B. cinerea from tobacco

杀菌剂 Fungicide	菌株 Strain	毒力回归方程 Virulence regression equation	相关系数 Correlation coefficient, r	EC₅₀/(mg/L)	EC₉₀/(mg/L)	EC₅₀ 平均值 Average EC₅₀/(mg/L)	EC₉₀ 平均值 Average EC₉₀/(mg/L)
多菌灵 carbendazim	3	y = 1.09x + 6.80	0.97	0.02	0.33	0.06 ± 0.04 b	0.97 ± 0.86 c
	3-2	y = 1.04x + 6.15	0.97	0.08	1.34
	4	y = 0.99x + 5.98	0.95	0.10	2.01
	6	y = 1.68x + 7.43	0.99	0.04	0.21
异菌脲 iprodione	3	y = 0.82x + 5.26	0.98	0.48	17.85	0.60 ± 0.25 a	10.53 ± 5.98 ab
	3-2	y = 1.52x + 5.49	0.98	0.47	3.30
	4	y = 0.94x + 5.29	0.98	0.49	11.33
	6	y = 1.28x + 5.02	0.97	0.97	9.64
嘧霉胺 pyrimethanil	3	y = 1.66x + 5.22	0.96	0.73	4.37	0.53 ± 0.28 a	3.68 ± 0.90 bc
	3-2	y = 1.73x + 5.15	0.89	0.22	3.24
	4	y = 1.11x + 5.72	0.91	0.81	4.47
	6	y = 1.52x + 5.65	0.93	0.37	2.62
丙环唑 propiconazole	3	y = 0.79x + 5.55	0.98	0.69	30.73	0.36 ± 0.27 ab	13.34 ± 11.97 a
	3-2	y = 0.78x + 5.12	0.98	0.21	8.40
	4	y = 0.61x + 5.65	0.98	0.09	10.67
	6	y = 1.45x + 5.49	0.98	0.46	3.54
注：表中数据为平均值 ± 标准差 (n = 3)，不同小写字母表示处理间存在显著性差异 (P < 0.05)。Note: Data in the table were average value ± standard deviation (n = 3). Different lowercase indicates significant differences among treatments with P < 0.05.

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表 2 供试4种杀菌剂对烟草灰霉病菌孢子萌发的抑制作用

Table 2. Inhibitory effects of four fungicides against the spore germination of B. cinerea from tobacco

杀菌剂 Fungicide	菌株 Strain	毒力回归方程 Virulence regression equation	相关系数 Correlation coefficient, r	EC₅₀/(mg/L)	EC₉₀/(mg/L)	EC₅₀ 平均值 Average EC₅₀/(mg/L)	EC₉₀ 平均值 Average EC₉₀/(mg/L)
多菌灵 carbendazim	3	y = 3.62x ‒ 1.85	0.98	78.31	177.03	131.23 ± 45.16 a	240.00 ± 45.24 a
	3-2	y = 4.75x ‒ 5.04	0.98	130.31	242.60
	4	y = 4.21x ‒ 3.87	0.98	127.60	257.06
	6	y = 4.13x ‒ 3.85	0.99	188.71	283.31
异菌脲 iprodione	3	y = 5.69x + 2.47	0.94	2.79	4.69	2.05 ± 0.57 b	3.64 ± 0.83 c
	3-2	y = 4.57x + 4.25	0.98	1.46	2.79
	4	y = 5.16x + 3.27	0.96	2.16	3.85
	6	y = 4.93x + 3.78	0.98	1.77	3.22
嘧霉胺 pyrimethanil	3	y = 2.04x + 2.67	0.98	13.96	59.36	10.56 ± 7.23 b	44.73 ± 28.62 b
	3-2	y = 1.73x + 4.09	0.99	3.36	18.55
	4	y = 1.99x + 3.57	0.99	5.26	23.18
	6	y = 2.15x + 2.22	0.97	19.67	77.81
丙环唑 propiconazole	3	y = 6.32x + 2.93	0.99	2.13	3.40	2.21 ± 0.48 b	4.73 ± 1.18 c
	3-2	y = 3.34x + 4.23	0.96	1.70	4.11
	4	y = 3.16x + 3.94	0.94	2.17	5.52
	6	y = 4.05x + 3.16	0.98	2.85	5.90
注：表中数据为平均值 ± 标准差 (n = 3)，不同小写字母表示处理间存在显著性差异 (P < 0.05)。Note: Data in the table were average value ± standard deviation (n = 3). Different lowercase indicates significant differences among treatments with P < 0.05.

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表 3 供试4种杀菌剂对离体叶片上烟草灰霉病的保护和治疗作用防效

Table 3. Protective and curative effects of four fungicides against tobacco grey mould on detached leaves

杀菌剂 Fungicide	作用方式 Mode of action	防效 Control effects/%
杀菌剂 Fungicide	作用方式 Mode of action	3.13 mg/L	12.5 mg/L	50 mg/L	200 mg/L	800 mg/L
多菌灵 carbendazim	保护作用 Protective	58.36 ± 3.43 b	84.36 ± 2.42 a	98.76 ± 1.14 a	100 ± 0 a	100 ± 0 a
多菌灵 carbendazim	治疗作用 Curative	40.45 ± 2.86 c	74.34 ± 2.45 b	95.32 ± 2.43 a	100 ± 0 a	100 ± 0 a
异菌脲 iprodione	保护作用 Protective	66.81 ± 2.65 a	85.92 ± 2.43 a	92.86 ± 1.76 a	98.32 ± 1.45 a	100 ± 0 a
异菌脲 iprodione	治疗作用 Curative	65.76 ± 1.12 a	83.61 ± 0.65 a	89.50 ± 0.46 b	91.81 ± 2.34 b	100 ± 0 a
嘧霉胺 pyrimethanil	保护作用 Protective	51.76 ± 3.58 b	64.26 ± 2.54 c	76.76 ± 2.43 c	91.20 ± 3.26 b	100 ± 0 a
嘧霉胺 pyrimethanil	治疗作用 Curative	5.46 ± 2.24 d	57.75 ± 3.34 cd	59.15 ± 3.56 d	62.68 ± 3.42 c	69.37 ± 3.12 b
丙环唑 propiconazole	保护作用 Protective	58.80 ± 3.48 b	65.49 ± 2.67 c	78.70 ± 1.48 c	94.72 ± 2.63 b	100 ± 0 a
丙环唑 propiconazole	治疗作用 Curative	10.56 ± 3.12 d	61.27 ± 1.34 c	64.61 ± 3.75 d	65.32 ± 4.36 c	74.82 ± 3.24 b
注：表中数据为平均值 ± 标准差 (n = 3)，不同小写字母表示处理间差异显著 (P < 0.05)。Note: Data in the table were average value ± standard deviation (n = 3). Different lowercase indicates significant differences among treatments with P < 0.05.

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参考文献(19)

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多菌灵等4种杀菌剂对烟草灰霉病菌的室内生物活性

doi: 10.16801/j.issn.1008-7303.2019.0033

作者简介:
^周浩，男，硕士研究生，E-mail：919355397@qq.com

^李丽翠，并列第一作者，女，硕士研究生，E-mail：2392693846@qq.com

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In vitro bioactivities of four fungicides including carbendazim against Botrytis cinerea in tobacco

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多菌灵等4种杀菌剂对烟草灰霉病菌的室内生物活性

doi: 10.16801/j.issn.1008-7303.2019.0033

作者简介: **周浩，男，硕士研究生，E-mail：919355397@qq.com **李丽翠，并列第一作者，女，硕士研究生，E-mail：2392693846@qq.com

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In vitro bioactivities of four fungicides including carbendazim against Botrytis cinerea in tobacco

计量

出版历程

目录

作者简介:
^周浩，男，硕士研究生，E-mail：919355397@qq.com

^李丽翠，并列第一作者，女，硕士研究生，E-mail：2392693846@qq.com