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两种海洋真菌混合发酵的次级代谢产物及杀虫活性研究

曹云 侯宗敏 董存柱 曹凤勤 陶敏 余森泉 夏玉莲 伍显锋

曹云, 侯宗敏, 董存柱, 曹凤勤, 陶敏, 余森泉, 夏玉莲, 伍显锋. 两种海洋真菌混合发酵的次级代谢产物及杀虫活性研究[J]. 农药学学报, 2022, 24(2): 423-428. doi: 10.16801/j.issn.1008-7303.2021.0179
引用本文: 曹云, 侯宗敏, 董存柱, 曹凤勤, 陶敏, 余森泉, 夏玉莲, 伍显锋. 两种海洋真菌混合发酵的次级代谢产物及杀虫活性研究[J]. 农药学学报, 2022, 24(2): 423-428. doi: 10.16801/j.issn.1008-7303.2021.0179
CAO Yun, HOU Zongmin, DONG Cunzhu, CAO Fengqin, TAO Min, YU Senquan, XIA Yulian, WU Xianfeng. Study on secondary metabolites and insecticidal activities of mixed fermentation of two marine fungi[J]. Chinese Journal of Pesticide Science, 2022, 24(2): 423-428. doi: 10.16801/j.issn.1008-7303.2021.0179
Citation: CAO Yun, HOU Zongmin, DONG Cunzhu, CAO Fengqin, TAO Min, YU Senquan, XIA Yulian, WU Xianfeng. Study on secondary metabolites and insecticidal activities of mixed fermentation of two marine fungi[J]. Chinese Journal of Pesticide Science, 2022, 24(2): 423-428. doi: 10.16801/j.issn.1008-7303.2021.0179

两种海洋真菌混合发酵的次级代谢产物及杀虫活性研究

doi: 10.16801/j.issn.1008-7303.2021.0179
基金项目: 农业部财政项目 “海南重大入侵生物-草地贪夜蛾与红火蚁监测与防控” (1630042020020).
详细信息
    作者简介:

    曹云,1779913607@qq.com

    通讯作者:

    董存柱,czd@hainanu.edu.cn.

  • 中图分类号: S482.3;O656.2

Study on secondary metabolites and insecticidal activities of mixed fermentation of two marine fungi

Funds: Major Invasive Species in Hainan—Monitoring and Control of Spodoptera frugiperda and Solenopsis invicta(1630042020020)
  • 摘要: 为了研究海洋真菌哈茨木霉Trichoderma harzianum ABC19819和短柄青霉菌Penicillium Brefeldianum ABC190807混合发酵产生的次级代谢产物,利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和薄层色谱等多种分离纯化手段对混合发酵所产生的次级代谢产物进行分离纯化,结合波谱分析技术(1H NMR、13C NMR、ESI-MS)及文献对比鉴定了化合物的结构。以埃及伊蚊Aedes aegypti 3龄幼虫为研究对象,对分离鉴定得到的化合物进行活性评价。结果表明,从2株真菌混合培养的发酵产物中分离得到6个已知化合物,分别鉴定为agathic acid ( 1 )、4-羟基苯甲醛 ( 2 )、nafuredin ( 3 )、4-羟基-3,6-二甲基-2H-吡喃酮 ( 4 )、甲瓦龙酸内酯 ( 5 )和brefeldin A ( 6 ),其中化合物 1 为首次由哈茨木霉与短柄青霉菌的混合发酵物中分离得到。化合物 13 对埃及伊蚊3龄幼虫表现出明显的毒杀活性,处理72 h后的LC50值分别为162 mg/L和240 mg/L。该研究对于通过混合发酵得到具有杀虫活性的化合物具有重要参考价值。
  • 1  化合物1~6的化学结构

    1.  Structures of compounds 1-6

    表  1  500 mg/L化合物1~6对埃及伊蚊3龄幼虫的杀虫活性

    Table  1.   The insecticidal activities of compounds 1-6 at 500 mg/L against the third instar larvae of Aedes aegypti ($ \overline {\boldsymbol X}{\bf \pm {SD}}$, n = 3)

    成分
    Compounds
    校正死亡率 ($ \overline { X}\pm { \rm {SD}}$, n = 3)
    Adjusted mortality rate/%
    12 h24 h36 h72 h
    1 73.33±7.64 b 83.33±2.89 b 86.67±2.89 b 100.00±0.00 a
    2 6.67±3.33 c
    3 58.33±2.89 c 70.00±5.00 c 78.33±5.77 c 81.66±2.89 b
    4
    5 8.33±2.89 c
    6
    CK
    鱼藤酮 rotenone 100.00±0.00 a 100.00±0.00 a 100.00±0.00 a 100.00±0.00 a
    注:同列数据后不同小写字母表示差异显著 (P<0.05,DMRT法) ;“—” 表示无毒杀作用。Note: Data followed different letters within colum indicate significant differences at 0.05 level. "—" indicate no larvicidal activity.
    下载: 导出CSV

    表  2  72 h化合物1和3对埃及伊蚊3龄幼虫的LC50

    Table  2.   LC50 value of compound 1 and 3 after 72 h against the third instar larvae of Aedes aegypti

    成分
    Compound
    毒力回归曲线
    Regression equation of toxicity
    相关系数
    Correlation coefficient
    LC50/(mg/L)95% 置信区间
    95% confidence interval /(mg/L)
    1 y = 2.1967x + 0.1413 0.9366 162 137~192
    3 y = 2.2440x − 0.3418 0.9700 240 205~280
    鱼藤酮 rotenone y = 1.4031x + 3.4167 0.9894 13.4 5.72~17.5
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-06-22
  • 录用日期:  2021-09-13
  • 网络出版日期:  2021-12-06
  • 刊出日期:  2022-04-01

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