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樱桃褐腐病菌对啶酰菌胺的敏感性及其对4种琥珀酸脱氢酶抑制剂的交互抗性

董怡 李阿根 毛程鑫 张艳婷 张传清 刘亚慧

董怡, 李阿根, 毛程鑫, 张艳婷, 张传清, 刘亚慧. 樱桃褐腐病菌对啶酰菌胺的敏感性及其对4种琥珀酸脱氢酶抑制剂的交互抗性[J]. 农药学学报, 2022, 24(2): 298-305. doi: 10.16801/j.issn.1008-7303.2021.0180
引用本文: 董怡, 李阿根, 毛程鑫, 张艳婷, 张传清, 刘亚慧. 樱桃褐腐病菌对啶酰菌胺的敏感性及其对4种琥珀酸脱氢酶抑制剂的交互抗性[J]. 农药学学报, 2022, 24(2): 298-305. doi: 10.16801/j.issn.1008-7303.2021.0180
DONG Yi, LI Agen, MAO Chengxin, ZHANG Yanting, ZHANG Chuanqing, LIU Yahui. Sensitivity of Monilinia fructicola to boscalid and cross-resistance to four succinate dehydrogenase inhibitors[J]. Chinese Journal of Pesticide Science, 2022, 24(2): 298-305. doi: 10.16801/j.issn.1008-7303.2021.0180
Citation: DONG Yi, LI Agen, MAO Chengxin, ZHANG Yanting, ZHANG Chuanqing, LIU Yahui. Sensitivity of Monilinia fructicola to boscalid and cross-resistance to four succinate dehydrogenase inhibitors[J]. Chinese Journal of Pesticide Science, 2022, 24(2): 298-305. doi: 10.16801/j.issn.1008-7303.2021.0180

樱桃褐腐病菌对啶酰菌胺的敏感性及其对4种琥珀酸脱氢酶抑制剂的交互抗性

doi: 10.16801/j.issn.1008-7303.2021.0180
基金项目: 浙江省重点研发项目 (2020C02005).
详细信息
    作者简介:

    董怡, 1262406417@qq.com

    通讯作者:

    张传清, cqzhang9603@126.com

    刘亚慧, liuyahui716@163.com.

  • 中图分类号: S481.4;TQ450.13

Sensitivity of Monilinia fructicola to boscalid and cross-resistance to four succinate dehydrogenase inhibitors

Funds: Key R & D Program in Zhejiang Province (2020C02005).
  • 摘要: 采用菌丝生长速率法测定了樱桃褐腐病菌Monilinia fructicola对啶酰菌胺的敏感性,同时研究了不同敏感性菌株的生物学性状,探究了琥珀酸脱氢酶B亚基的氨基酸突变与其对啶酰菌胺产生抗性的相关性,并分析了樱桃褐腐病菌对啶酰菌胺与其他3种琥珀酸脱氢酶抑制剂 (SDHIs)氯苯醚酰胺、氟唑菌苯胺和氟吡菌酰胺之间的交互抗性。结果表明:啶酰菌胺对樱桃褐腐病菌具有较好的抑制活性和治疗作用,但樱桃褐腐病菌已对其产生了一定的抗性,且抗性菌株具有较高的适合度。褐腐病菌SDHB亚基上的氨基酸点突变与其对啶酰菌胺的抗性之间无明显联系。交互抗药性分析表明,啶酰菌胺与氯苯醚酰胺、氟唑菌苯胺和氟吡菌酰胺之间均存在交互抗性。为了延缓抗药性的发生和发展,在樱桃褐腐病防治过程中啶酰菌胺应与SDHIs之外的其他类型杀菌剂进行合理的混用或轮用。
  • 图  1  樱桃褐腐病菌 (n=28) 对啶酰菌胺的敏感性

    Figure  1.  The sensitivity of M. fructicola (n=28) to boscalid

    图  2  樱桃褐腐病菌 (n=28) 对啶酰菌胺的抗性倍数分布

    Figure  2.  The distribution of resistance ratio of M. fructicola (n=28) to boscalid

    图  3  樱桃褐腐病菌 (n=28) 对氯苯醚酰胺、氟唑菌苯胺和氟吡菌酰胺的敏感性(EC50区间)分布

    Figure  3.  Distribution profile of M. fructicola sensitivity (EC50) to Y12196, penflufen and fluopyram (n=28)

    图  4  樱桃褐腐病菌中啶酰菌胺与氯苯醚酰胺 (A) 、氟唑菌苯胺 (B) 和氟吡菌酰胺 (C) 的交互抗性

    Figure  4.  Cross-resistance between boscalid and Y12196 (A), penflufen (B) and fluopyram (C) in M. fructicola

    表  1  用于扩增美澳型核果链核盘菌SDHB基因的引物

    Table  1.   Primers used for the amplification of SDHB in M. fructicola

    编号
    Serial number
    序列
    Sequence (5′-3′)
    长度
    Length/bp
    目的基因
    Target gene
    CQ110ATCTCTCCGCACCACCAG884SDHB
    CQ111CTTGATCTCCGCAATCGCC
    下载: 导出CSV

    表  2  啶酰菌胺对樱桃褐腐病的防治效果

    Table  2.   The control effects of boscalid on cherry brown rot

    菌株
    Isolate
    啶酰菌胺质量浓度
    Mass concentration of boscalid/(µg/mL)
    防治效果
    Control efficacy/%
    Mf19-3-8S 10 0.01±7.17 d
    50 76.75±8.70 b
    100 100.00±0.00 a
    Mf19-3-1LR 10 −1.09±4.61 d
    50 100.00±0.00 a
    100 100.00±0.00 a
    Mf19-1-7LR 10 10.64±5.21 d
    50 88.30±10.53 ab
    100 100.00±0.00 a
    Mf19-5-10MR 10 6.74±1.59 d
    50 33.71±5.73 c
    100 88.76±9.67 ab
    注:S代表啶酰菌胺敏感菌株,LR代表啶酰菌胺低抗菌株,MR代表啶酰菌胺中抗菌株。*同列数据后不同字母表示该列所有处理的防治效果在P=0.05水平显著性差异。“±” 代表3次重复的防治效果之间的标准误差。Note: The superscript letter "S" adjacent to the isolate number represents boscalid-sensitivity. "LR" represents boscalid low-resistance. "MR" represents boscalid medium-resistance. *The values in a column followed by different lowercase letters indicate the control efficacy of all treatments is significantly different at the P =0.05 level. "±" represents the standard error between three repetitions of control efficacy.
    下载: 导出CSV

    表  3  对啶酰菌胺不同敏感性的樱桃褐腐病菌的生物学特性

    Table  3.   Biological characteristics of M. fructicola with different sensitivities to boscalid

    菌株
    Isolate
    平均生长速率
    Average growth rate/(mm/d)
    产孢量
    Sporulation quantity/
    ( × 106 个/mL)
    孢子萌发率
    Spore germination rate/%
    0.5 h1.5 h2.5 h
    Mf19-4-7S 10.27±0.17 a 1.05±0.86 cde 23.44±4.94 b 52.28±1.46 bc 86.81±2.93 ab
    Mf19-3-7S 9.71±0.67 c 0.75±0.55 de 11.81±0.95 b 32.90±5.00 c 62.91±8.28 c
    Mf19-3-8S 10.25±0.29 ab 0.40±0.51 e 74.55±12.77 a 96.67±5.60 a 100.00±3.12 a
    Mf19-1-7LR 9.96±0.88 bc 2.50±1.26 a 17.83±5.05 b 60.43±4.60 b 90.28±1.12 ab
    Mf19-3-1LR 10.27±0.17 a 2.05±1.03 abc 6.86±4.73 b 42.18±3.33 bc 80.46±0.00 bc
    Mf19-4-6MR 9.97±0.53 bc 0.07±0.17 f
    Mf19-5-9MR 10.21±0.33 ab 2.05±0.37 ab 13.85±3.03 b 62.25±2.64 b 83.80±1.96 ab
    Mf19-5-10MR 10.21±0.44 ab 1.55±0.80 bcd 0.95±1.17 b 36.22±4.90 c 80.66±3.65 bc
    注:S代表啶酰菌胺敏感菌株,LR代表啶酰菌胺低抗菌株,MR代表啶酰菌胺中抗菌株。*同列数据后不同字母表示在P=0.05水平显著性差异。“±” 代表3次重复的平均生长速率、产孢量及孢子萌发率的标准误。Note: The superscript letter "S" adjacent to the isolate number represents boscalid-sensitivity. "LR" represents boscalid low-resistance. "MR" represents boscalid medium-resistance. *Values in a column followed by different lowercase letters are significantly different at P = 0.05 level. "±" represents the standard error among three repetitions of average growth rates, sporulation quantities, and spore germination rates.
    下载: 导出CSV

    表  4  樱桃褐腐病菌对啶酰菌胺的EC50及氨基酸突变位点

    Table  4.   EC50 of boscalid and amino acid mutation sites in M. fructicola

    菌株
    Isolate
    EC50/(µg/mL)氨基酸突变
    Mutation of amino acid
    Mf19-3-7S 1.05
    Mf19-3-8S 1.30 I117V,R237P
    Mf19-3-10S 1.34
    Mf19-4-7S 2.65 A133V,R237P
    Mf19-4-10S 1.02
    Mf19-1-7LR 10.17
    Mf19-3-1LR 5.68 A133V,R237P
    Mf19-4-6MR 25.14
    Mf19-5-9MR 17.761 A133V,R237P
    Mf19-5-10MR 30.59 A133V,R237P
    注:S代表啶酰菌胺敏感菌株,LR代表啶酰菌胺低抗菌株,MR代表啶酰菌胺中抗菌株。“—” 表示该菌株中未发生氨基酸突变。Note: The superscript letter "S" adjacent to the isolate number represents boscalid-sensitivity. "LR" represents boscalid low-resistance. "MR" represents boscalid medium-resistance. "—" means that no amino acid point mutations had occurred in this strain.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-12-29
  • 录用日期:  2021-11-04
  • 网络出版日期:  2021-12-08
  • 刊出日期:  2022-04-10

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