Influence of abscisic acid functional analog natenpac on grape coloring and quality improvement
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摘要: 葡萄着色是葡萄生长过程中的重要环节,对葡萄品质有极为重要的影响。本文以9年生弗雷葡萄品种为对象,研究了新型脱落酸功能类似物萘酮戊酸(natenpac)对田间葡萄果实着色及品质提升的影响。在果实着色初期,以萘酮戊酸有效成分2、5、10 mg/L 3个质量浓度梯度分别进行叶面喷雾、灌根或浸果处理,以清水处理为空白对照。通过对果实平均横径、颜色、单果重等表观变化以及可溶性固形物、花青素、可溶性糖含量等内在品质的变化进行综合分析,以确定最佳的施药方式和浓度。结果表明:以叶面喷雾和灌根处理,萘酮戊酸可使弗雷葡萄较对照提前7~10 d完成着色,显著提高了果实中可溶性固形物含量和着色程度,同时浆果横径和单果重也略有提高。不同处理方式对使用效果影响显著,灌根和叶面喷雾效果优于浸果,其中以叶面喷雾2 mg/L的处理效果最佳。本研究明确了萘酮戊酸对弗雷葡萄的最佳施药方式和剂量,可为指导萘酮戊酸在葡萄着色中的田间应用提供依据。Abstract: Grape coloring is an essential stage in the grape ripening process, which has a very important influence on the quality of grape berries. In this paper, nine-year-old grape variety Frey was used as the research object and the influences of a novel abscisic acid (ABA) functional analog, natenpac, on grape coloring and quality improvement in the field were studied. At the initial stage of fruit coloring, foliar spraying, root irrigation, and fruit soaking were carried out with 2, 5, and 10 mg/L natenpac, respectively, and clean water treatment as the blank control. By comparing the apparent changes, such as average transverse diameter, color, and single fruit weight, and internal quality changes, such as soluble solids, anthocyanin, and soluble sugar contents, the optimal application mode and concentration were determined. The results showed that natenpac could make Frey grape finish coloring 7-10 days earlier than the control by foliar spraying or root soaking modes, and contributed to significant increases in soluble solid content and coloring degree, and slight increases in the transverse diameter and single fruit weight of berries. The three treatment modes showed a great difference in efficacy. Root irrigation and foliar spraying were better than fruit soaking and 2 mg/L of foliar spraying was the best. This study clarified the best application mode and concentration of natenpac to Frey grape and provided the basis for guiding the field application of natenpac in grape coloring.
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Key words:
- abscisic acid functional analog /
- natenpac /
- grape coloring /
- anthocyanin /
- soluble sugars
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表 1 不同处理下弗雷葡萄浆果的颜色变化
Table 1. Color changes of Frey grape berries under different treatments
处理方式 Treatment
mode有效成分质量浓度Active
ingredients concentration/ (mg/L)取样时间
Sampling time/d0 4 8 12 16 20 空白对照
CK0 叶面喷雾
Foliar spraying2 5 10 灌根
Root irrigation2 5 10 浸果
Fruit soaking2 5 10 表 2 萘酮戊酸对葡萄表观指标的影响
Table 2. Effect of natenpac on quality indicators of grapes
处理方式
Treatment mode横径
Transverse diameter/mm单果重
Mass per fruit/g色泽指数
CIRG2 mg/L 叶面喷雾 Foliar spraying 2 mg/L 16.45 ± 0.38 a 2.84 ± 0.17 a 5.19 ± 0.14 a 5 mg/L 叶面喷雾 Foliar spraying 5 mg/L 15.66 ± 0.51 b 2.43 ± 0.20 bc 5.25 ± 0.18 a 10 mg/L 叶面喷雾 Foliar spraying 10 mg/L 15.77 ± 0.23 b 2.56 ± 0.10 b 4.89 ± 0.09 b 2 mg/L 灌根 Root irrigation 2 mg/L 15.82 ± 0.13 b 2.45 ± 0.19 bc 4.59 ± 0.12 c 5 mg/L 灌根 Root irrigation 5 mg/L 15.76 ± 0.30 b 2.35 ± 0.14 bc 5.03 ± 0.10 ab 10 mg/L 灌根 Root irrigation 10 mg/L 15.70 ± 0.24 b 2.45 ± 0.12 bc 4.89 ± 0.12 b 2 mg/L 浸果 Fruit soaking 2 mg/L 15.83 ± 0.07 b 2.46 ± 0.03 bc 4.25 ± 0.07 d 5 mg/L 浸果 Fruit soaking 5 mg/L 15.76 ± 0.19 b 2.52 ± 0.14 bc 4.58 ± 0.23 c 10 mg/L 浸果 Fruit soaking 10 mg/L 15.66 ± 0.16 b 2.36 ± 0.07 bc 4.65 ± 0.15 c 空白对照 CK 15.62 ± 0.15 b 2.34 ± 0.11 c 4.42 ± 0.19 cd 注:表2为处理后20 d葡萄各指标变化。同列不同小写字母表示在 0.05 水平上差异显著。Note: Table 2 shows the changes of each index of the grape 20 days after treatments. Different lowercase letters in the same column indicate significant differences at the 0.05 level. 表 3 萘酮戊酸对葡萄内在指标的影响
Table 3. Effect of natenpac on grape internal indexes
处理方式
Treatment mode可溶性固形物含量
Soluble solid content/%花青素含量
Anthocyanin content/(μg/g)可溶性糖含量
Soluble sugar content/(μg/g)2 mg/L 叶面喷雾 Foliar spraying 2 mg/L 17.08 ± 0.39 2.56 ± 0.07 202.97 ± 3.55 5 mg/L 叶面喷雾 Foliar spraying 5 mg/L 18.00 ± 0.45 2.49 ± 0.32 233.96 ± 11.66 10 mg/L 叶面喷雾 Foliar spraying 10 mg/L 16.78 ± 0.19 2.43 ± 0.13 227.68 ± 10.71 2 mg/L 灌根 Root irrigation 2 mg/L 16.52 ± 0.36 2.28 ± 0.17 242.43 ± 18.94 5 mg/L 灌根 Root irrigation 5 mg/L 17.50 ± 0.31 2.35 ± 0.11 253.87 ± 21.85 10 mg/L 灌根 Root irrigation 10 mg/L 17.70 ± 0.45 2.48 ± 0.24 259.08 ± 20.84 2 mg/L 浸果 Fruit soaking 2 mg/L 16.58 ± 0.54 2.23 ± 0.17 193.87 ± 21.52 5 mg/L 浸果 Fruit soaking 5 mg/L 16.88 ± 0.27 2.33 ± 0.18 182.90 ± 17.54 10 mg/L 浸果 Fruit soaking 10 mg/L 17.64 ± 0.47 2.27 ± 0.14 191.59 ± 14.14 空白对照 CK 15.54 ± 0.52 2.28 ± 0.13 192.26 ± 11.62 注:表3为第20天时葡萄各指标变化。Note: Table 3 shows the changes of each index 20 days after treatments. -
[1] POJER E, MATTIVI F, JOHNSON D, et al. The case for anthocyanin consumption to promote human health: a review[J]. Compr Rev Food Sci Food Saf, 2013, 12(5): 483-508. doi: 10.1111/1541-4337.12024 [2] SUN J J, WANG Y C, CHEN X S, et al. Effects of methyl jasmonate and abscisic acid on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f. niedzwetzkyana)[J]. Plant Cell Tissue Organ Cult, 2017, 130(2): 227-237. doi: 10.1007/s11240-017-1217-4 [3] FERRARA G, MAZZEO A, MATARRESE A M S, et al. Application of abscisic acid (S-ABA) and sucrose to improve colour, anthocyanin content and antioxidant activity of cv. Crimson Seedless grape berries[J]. Aust J Grape Wine Res, 2015, 21(1): 18-29. doi: 10.1111/ajgw.12112 [4] PIRIE A, MULLINS M G. Changes in anthocyanin and phenolics content of grapevine leaf and fruit tissues treated with sucrose, nitrate, and abscisic acid[J]. Plant Physiol, 1976, 58(4): 468-472. doi: 10.1104/pp.58.4.468 [5] WHEELER S, LOVEYS B, FORD C, et al. The relationship between the expression of abscisic acid biosynthesis genes, accumulation of abscisic acid and the promotion of Vitis vinifera L. berry ripening by abscisic acid[J]. Aust J Grape Wine Res, 2009, 15(3): 195-204. doi: 10.1111/j.1755-0238.2008.00045.x [6] OH H D, YU D J, CHUNG S W, et al. Abscisic acid stimulates anthocyanin accumulation in 'Jersey' highbush blueberry fruits during ripening[J]. Food Chem, 2018, 244: 403-407. doi: 10.1016/j.foodchem.2017.10.051 [7] GAGNÉ S, CLUZET S, MÉRILLON J M, et al. ABA initiates anthocyanin production in grape cell cultures[J]. J Plant Growth Regul, 2011, 30(1): 1-10. doi: 10.1007/s00344-010-9165-9 [8] JU Y L, LIU M, ZHAO H, et al. Effect of exogenous abscisic acid and methyl jasmonate on anthocyanin composition, fatty acids, and volatile compounds of Cabernet Sauvignon (Vitis vinifera L.) grape berries[J]. Molecules, 2016, 21(10): 1354. doi: 10.3390/molecules21101354 [9] 韩小强. 新型脱落酸类似物的合成及生物学评价[D]. 北京: 中国农业大学, 2014.HAN X Q. Synthesis and biological evaluation of novel abscisic acid analogs[D]. Beijing: China Agricultural University, 2014. [10] 覃兆海, 韩小强, 万川, 等. 一类高活性苯并异脱落酸类似物及其制备方法: CN 103435472A[P]. 2013-12-11.QIN Z H, HAN X Q, WAN C, et al. A class of highly active benzoisoabscisic acid analogs and preparation method thereof: CN 103435472A[P]. 2013-12-11. [11] 丁珊珊. 新型植物生长调节剂萘酮戊酸的应用基础研究[D]. 北京: 中国农业大学, 2020.DING S S. Study on the application foundation of natenpac as a new plant growth regulator[D]. Beijing: China Agricultural University, 2020. [12] VERGARA A E, DÍAZ K, CARVAJAL R, et al. Exogenous applications of brassinosteroids improve color of red table grape (Vitis vinifera L. cv. “redglobe”) berries[J]. Front Plant Sci, 2018, 9: 363. doi: 10.3389/fpls.2018.00363 [13] CARREÑO J, MARTÍNEZ A, ALMELA L, et al. Proposal of an index for the objective evaluation of the colour of red table grapes[J]. Food Res Int, 1995, 28(4): 373-377. doi: 10.1016/0963-9969(95)00008-A [14] 陈宽, 史蛟华, 安梦洁, 等. 叶面调节剂对新疆鲜食葡萄着色及品质的影响[J]. 新疆农业科学, 2021, 58(4): 599-606.CHEN K, SHI J H, AN M J, et al. Effects of foliar regulators on color and quality of table grapes in Xinjiang[J]. Xinjiang Agric Sci, 2021, 58(4): 599-606. [15] 王爱玲, 白世践, 赵荣华, 等. 油菜素内酯对火焰无核葡萄着色的影响[J]. 天津农业科学, 2019, 25(1): 34-35. doi: 10.3969/j.issn.1006-6500.2019.01.008WANG A L, BAI S J, ZHAO R H, et al. Effects of brassinolide on colour of flame seedless grape[J]. Tianjin Agric Sci, 2019, 25(1): 34-35. doi: 10.3969/j.issn.1006-6500.2019.01.008 [16] BALATE C A, CORREA de SOUZA D, SILVA L F L, et al. Abscisic acid on the quality of tomato fruits[J]. Sci Agrar Paranaensis, 2020, 19(1): 38. doi: 10.18188/sap.v19i1.22876 [17] 马文瑶. ABA和PDJ对葡萄果实着色及品质的影响[D]. 洛阳: 河南科技大学, 2019.MA W Y. Effects of ABA and PDJ on coloration and quality of grape berries[D]. Luoyang: Henan University of Science and Technology, 2019. [18] CARDOSO D S C P, MARTINEZ H E P, PEREIRA A M, et al. Potassium and growth-promoting fungi improve the postharvest quality of grape tomato[J]. Semina: Ciênc Agrár, 2022, 43(2): 675-692. [19] BASILE T, MARSICO A D, PERNIOLA R. Use of artificial neural networks and NIR spectroscopy for non-destructive grape texture prediction[J/OL]. Foods, 2022, 11(3): 281. https://doi.org/10.3390/foods11030281. -