上海市水稻田千金子对3种乙酰辅酶A羧化酶抑制剂的抗性现状及酶突变机制

袁国徽; 田志慧; 高原; 沈国辉

doi:10.16801/j.issn.1008-7303.2022.0012

上海市水稻田千金子对3种乙酰辅酶A羧化酶抑制剂的抗性现状及酶突变机制

doi: 10.16801/j.issn.1008-7303.2022.0012

袁国徽^{1, 2,},
田志慧^{1, 2},
高原^{1, 2},
沈国辉^{1, 2, ,}

1.
上海市农业科学院生态环境保护研究所，上海 201403
2.
上海市设施园艺技术重点实验室，上海 201403

基金项目: 上海市科技兴农重点攻关项目(2020-02-08-00-12-F01490)；上海市农业科学院卓越团队项目(农科创2018 B-01)

详细信息

作者简介:
袁国徽，mypostok@126.com

通讯作者:
沈国辉，ghshen65@163.com

中图分类号: S481.4；S482.4
计量
- 文章访问数: 17
- HTML全文浏览量: 7
- 被引次数: 0
出版历程
- 收稿日期: 2021-09-22
- 录用日期: 2022-02-07
- 网络出版日期: 2022-05-10

Resistance status of Leptochloa chinensis to three acetyl-CoA carboxylase (ACCase) inhibitors in rice fields in Shanghai and involved ACCase gene mutations

YUAN Guohui^{1, 2
,},
TIAN Zhihui^{1, 2},
GAO Yuan^{1, 2},
SHEN Guohui^{1, 2
, ,}

1.
Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
2.
Key Laboratory of Protected Horticultural Technology, Shanghai 201403, China

Funds: Shanghai Agriculture Applied Technology Development Program (2020-02-08-00-12-F01490); Shanghai Academy of Agricultural Sciences for the Program of Excellent Research Team (2018 B-01).

摘要

摘要: 为明确上海市水稻田千金子对乙酰辅酶A羧化酶 (ACCase) 抑制剂类除草剂的抗性发生情况及可能存在的抗性机制，在上海市千金子发生严重地区的水稻田共采集51个种群，采用单剂量抗性甄别法测定了不同千金子种群对3种ACCase抑制剂类除草剂的抗性水平，扩增和比对了靶标酶ACCase基因部分片段的差异。结果显示：在氰氟草酯105 g/hm²有效成分剂量选择压下，8个千金子种群可标记为抗性种群，6个种群标记为发展中抗性种群；在噁唑酰草胺120 g/hm²有效成分剂量选择压下，4个千金子种群标记为抗性种群，5个种群为发展中抗性种群；在精噁唑禾草灵62.1 g/hm²有效成分剂量选择压下，6个千金子种群标记为抗性种群，2个种群为发展中抗性种群。对15个抗性千金子种群靶标酶基因片段的测序发现，9个种群发生了4种ACCase基因突变类型，分别为ACCase基因 1999 位点色氨酸 (TGG) 突变为丝氨酸 (TCG) 、1999位点色氨酸 (TGG) 突变为半胱氨酸 (TGT) 、2027位点色氨酸 (TGG) 突变为丝氨酸 (TCG) 以及2027位点色氨酸 (TGG) 突变为半胱氨酸 (TGT/TGC)。其中，5个抗性千金子种群ACCase 基因突变频率均大于60%。研究表明，ACCase抑制剂类除草剂抗性千金子在上海市部分地区发生较为严重，ACCase 基因突变是导致不同千金子种群对该类除草剂产生抗性的重要原因之一。
- 千金子 /
- 乙酰辅酶A羧化酶 /
- 氰氟草酯 /
- 噁唑酰草胺 /
- 精噁唑禾草灵 /
- 靶标抗性 /
- 基因突变
Abstract: In order to determine the resistance level and the potential resistance mechanism of Leptochloa chinensis to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides, 51 populations of L. chinensis were collected from the rice fields in Shanghai. Whole-plant single dose experiments were conducted to investigate the resistance level of different populations to ACCase-inhibiting herbicides such as cyhalofop-butyl at 105 a.i./(g/hm²), metamifop at 120 a.i./(g/hm²) and fenoxaprop-P-ethyl at 62.5 a.i./(g/hm²) in the greenhouse, and the target gene of resistant populations were detected. The results showed that of the 51 populations tested, eight populations were found to be resistant to clodinafop-propargyl, four populations resistant to metamifop, and six populations resistant to fenoxaprop-P-ethyl; six, five and two populations were found to be developing resistance to cyhalofop-butyl, metamifop and fenoxaprop-P-ethyl, respectively. Sequencing results showed that nine of fifteen resistant popualtions were found four different kinds of mutation at ACCase sequence included Trp (TGG)-1999-Ser (TCG), Trp (TGG)-1999-Cys (TGT), Trp (TGG)-2027-Ser (TCG) and Trp (TGG)-2027-Cys (TGT/TGC), but there were no mutations in other populations. Besides, five populations had mutant frequencies higher than 60%. Our results indicated that L. chinensis was extensively resistant to ACCase-inhibiting herbicides in some rice fields in Shanghai, and the mutation in the ACCase gene was one of main reasons for the resistance to ACCase-inhibiting herbicides in L. chinensis populations.
- Leptochloa chinensis /
- acetyl-CoA carboxylase /
- cyhalofop-butyl /
- metamifop /
- fenoxaprop-P-ethyl /
- target-site resistance /
- gene mutation

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表 1 千金子种群的采集地点

Table 1. Source locations of the Leptochloa chinensis populations examined in this study

种群 Population	采集地点 Sampling site	经纬度 Longitude and latitude	种群 Population	采集地点 Sampling site	经纬度 Longitude and latitude
2017-CM1	崇明区直河村 Zhihe Village, Chongming District	121.5996°E; 31.6425°N	2017-JS1	金山区曾家圩 Zengjia Village, Jinshan District	121.1790°E; 30.7878°N
2017-CM2	崇明区三官村 Sanguan Village, Chongming District	121.2996°E; 31.7893°N	2017-JS2	金山区百家村 Baijia Village, Jinshan District	121.2835°E; 30.8238°N
2017-CM3	崇明区江平村 Jiangping Village, Chongming District	121.5320°E; 31.6678°N	2017-JS3	金山区山塘村 Shantang Village, Jinshan District	121.1722°E; 30.7819°N
2017-CM4	崇明区立新村 Lixin Village, Chongming District	121.9090°E; 31.5271°N	2017-JS4	金山区蔡家村 Caijia Village, Jinshan District	121.0215°E; 30.8771°N
2017-CM5	崇明区定南村 Dingnan Village, Chongming District	121.4042°E; 31.7268°N	2017-JS5	金山区秦阳村 Zengjia Village, Jinshan District	121.2661°E; 30.7876°N
2017-CM6	崇明区新民村 Xinmin Village, Chongming District	121.5592°E; 31.6187°N	2017-JS6	金山区镇南村 Zhennan Village, Jinshan District	121.4013°E; 30.7887°N
2017-CM7	崇明区新海农场 Xinhai Farm, Chongming District	121.2436°E; 31.8152°N	2017-JS7	金山区建国村 Jianguo Village, Jinshan District	121.4036°E; 30.8163°N
2017-CM8	崇明区西新村 Xixin Village, Chongming District	121.2657°E; 31.7850°N	2017-MH1	闵行区联民村 Lianmin Village, Minhang District	121.4958°E; 31.0492°N
2017-CM9	崇明区富军村 Fujun Village, Chongming District	121.6754°E; 31.6187°N	2017-PD1	浦东新区会龙村 Huilong Village, Pudong New Area	121.7161°E; 31.1000°N
2016-FX1	奉贤区金港村 Jingang Village, Fengxian District	121.4575°E; 30.9869°N	2017-PD2	浦东新区万宝村 Wanbao Village, Pudong New Area	121.8228°E; 30.9620°N
2016-FX2	奉贤区北阮家 Beiruan Village, Fengxian District	121.4053°E; 30.9342°N	2017-PD3	浦东新区牌楼村 Pailou Village, Pudong New Area	121.5584°E; 31.0577°N
2017-FX1	奉贤区韩村村 Hancun Village, Fengxian District	121.4714°E; 30.9533°N	2017-PD4	浦东新区成日村 Chengri Village, Pudong New Area	121.8305°E; 31.0597°N
2017-FX2	奉贤区包家弄 Baojia Village, Fengxian District	121.4717°E; 30.9611°N	2017-PD5	浦东新区新兴村 Xinxing Village, Pudong New Area	121.7302°E; 31.0992°N
2017-FX3	奉贤区屠家村 Tujia Village, Fengxian District	121.4781°E; 30.9708°N	2017-PD6	浦东新区新如村 Xinru Village, Pudong New Area	121.8174°E; 31.0710°N
2017-FX4	奉贤区公谊村 Gongyi Village, Fengxian District	121.4636°E; 30.9644°N	2017-PD7	浦东新区森林村 Senlin Village, Pudong New Area	121.7377°E; 31.2114°N
2016-JD1	嘉定区金家村 Jinjia Village, Jiading District	121.2917°E; 31.4414°N	2017-PD8	浦东新区棉场村 Mianchang Village, Pudong New Area	121.8469°E; 30.9915°N
2016-JD2	嘉定区南村村 Nancun Village, Jiading District	121.2992°E; 31.4075°N	2017-PD9	浦东新区卫民村 Weimin Village, Pudong New Area	121.7406°E; 31.0987°N
2016-JD3	嘉定区葛隆村 Gelong Village, Jiading District	121.1547°E; 31.3928°N	2016-QP1	青浦区张巷村 Zhangxiang Village, Pudong New Area	121.0733°E; 31.0778°N
2016-JD4	嘉定区赵巷村 Zhaoxiang Village, Jiading District	121.2158°E; 31.3039°N	2016-QP2	青浦区安庄村 Anzhuang Village, Qingpu District	121.0394°E; 31.0772°N
2016-JD5	嘉定区曹家宅 Caojia Village, Jiading District	121.2992°E; 31.3992°N	2016-QP3	青浦区马塘村 Matang Village, Qingpu District	121.0428°E; 30.9803°N
2017-JD1	嘉定区汤家宅 Tangjia Village, Jiading District	121.3147°E; 31.4839°N	2017-QP1	青浦区莲湖村 Lianhu Village, Qingpu District	120.9771°E; 31.0663°N
2017-JD2	嘉定区梁卢宅 Lianglu Village, Jiading District	121.2667°E; 31.4447°N	2017-QP2	青浦区岑卜村 Cenbu Village, Qingpu District	120.9376°E; 31.0589°N
2017-JD3	嘉定区长塘村 Changtang Village, Jiading District	121.3058°E; 31.4453°N	2017-QP3	青浦区三和村 Sanhe Village, Qingpu District	120.8952°E; 31.0528°N
2017-JD4	嘉定区娄塘村 Loutang village, Jiading district	121.2094°E; 31.4361°N	2016-SJ1	松江区查家浜 Chajia Village, Songjiang District	121.0483°E; 30.9356°N
2017-JD5	嘉定区旺泾村 Wangjing Village, Jiading District	121.1819°E; 31.4183°N	2016-SJ2	松江区南泖村 Nanmao Village, Songjiang District	121.1414°E; 30.9464°N
2017-JD6	嘉定区灯塔村 Dengta Village, Jiading District	121.1644°E; 31.4350°N

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表 2 不同千金子种群在氰氟草酯105 g/hm²有效成分剂量处理下的存活率和抗性分类

Table 2. The survival rate and resistant category of the Leptochloa chinensis populations against cyhalofop-butyl at 105 a.i./(g/hm²)

种群 Population	存活率 Survival rate/%	抗性分类^a Resistant category	种群 Population	存活率 Survival rate/%	抗性分类^a Resistant category
2016-FX1	0	S	2017-JD2	10.0	DR
2016-FX2	0	S	2017-JD3	18.3	DR
2016-JD1	100	R	2017-JD4	59.2	R
2016-JD2	90.0	R	2017-JD5	79.2	R
2016-JD3	0	S	2017-JD6	0	S
2016-JD4	0	S	2017-JS1	16.7	DR
2016-JD5	4.2	DR	2017-JS2	23.3	R
2016-QP1	0	S	2017-JS3	0	S
2016-QP2	0	S	2017-JS4	0	S
2016-QP3	0	S	2017-JS5	0	S
2016-SJ1	0	S	2017-JS6	0	S
2016-SJ2	0	S	2017-JS7	0	S
2017-CM1	60.0	R	2017-MH1	0	S
2017-CM2	0	S	2017-PD1	2.5	DR
2017-CM3	32.5	R	2017-PD2	0	S
2017-CM4	0	S	2017-PD3	0	S
2017-CM5	0	S	2017-PD4	0	S
2017-CM6	0	S	2017-PD5	9.2	DR
2017-CM7	0	S	2017-PD6	0	S
2017-CM8	0	S	2017-PD7	0	S
2017-CM9	96.7	R	2017-PD8	0	S
2017-FX1	0	S	2017-PD9	0	S
2017-FX2	0	S	2017-QP1	0	S
2017-FX3	0	S	2017-QP2	0	S
2017-FX4	0	S	2017-QP3	0	S
2017-JD1	0	S
注：^a 敏感种群 (S)：存活率<1%；发展中抗性种群 (DR)：存活率在1%~20%之间；抗性种群 (R)：存活率>20%。Note: ^a Susceptible (S) population: Survival rate<1%; development of resistance (DR) population: 1%≤Survival rate≤20%; resistant (R) population: Survival rate>20%.

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表 3 不同千金子种群在噁唑酰草胺120 g/hm²有效成分剂量下的存活率和抗性分类

Table 3. The survival rate and resistant category of the Leptochloa chinensis populations against metamifop at 120 a.i./(g/hm²)

种群 Population	存活率 Survival rate/%	抗性分类^a Resistant category	种群 Population	存活率 Survival rate/%	抗性分类^a Resistant category
2016-FX1	0	S	2017-JD2	5.0	DR
2016-FX2	0	S	2017-JD3	0	S
2016-JD1	62.5	R	2017-JD4	0	S
2016-JD2	100	R	2017-JD5	6.7	DR
2016-JD3	0	S	2017-JD6	0	S
2016-JD4	0	S	2017-JS1	0	S
2016-JD5	0	S	2017-JS2	0	S
2016-QP1	0	S	2017-JS3	0	S
2016-QP2	0	S	2017-JS4	0	S
2016-QP3	5.8	DR	2017-JS5	0	S
2016-SJ1	0	S	2017-JS6	0	S
2016-SJ2	0	S	2017-JS7	0	S
2017-CM1	28.3	R	2017-MH1	0	S
2017-CM2	0	S	2017-PD1	0	S
2017-CM3	5.0	DR	2017-PD2	0	S
2017-CM4	0	S	2017-PD3	0	S
2017-CM5	0	S	2017-PD4	0	S
2017-CM6	0	S	2017-PD5	3.3	DR
2017-CM7	0	S	2017-PD6	0	S
2017-CM8	0	S	2017-PD7	0	S
2017-CM9	45.0	R	2017-PD8	0	S
2017-FX1	0	S	2017-PD9	0	S
2017-FX2	0	S	2017-QP1	0	S
2017-FX3	0	S	2017-QP2	0	S
2017-FX4	0	S	2017-QP3	0	S
2017-JD1	0	S
注：^a敏感种群 (S)：存活率<1%；发展中抗性种群 (DR)：存活率在1%~20%之间；抗性种群 (R)：存活率>20%。Note: ^a Susceptible (S) population: Survival rate<1%; development of resistance (DR) population: 1%≤Survival rate≤20%; resistant (R) population: Survival rate>20%.

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表 4 不同千金子种群在精噁唑禾草灵62.1 g/hm²有效成分剂量下的存活率和抗性分类

Table 4. The survival rate and resistant category of the Leptochloa chinensis populations against fenoxaprop-P-ethyl at 62.1 a.i./(g/hm²)

种群 Population	存活率 Survival rate/%	抗性分类^a Resistant category	种群 Population	存活率 Survival rate/%	抗性分类^a Resistant category
2016-FX1	0	S	2017-JD2	35.8	R
2016-FX2	0	S	2017-JD3	0	S
2016-JD1	61.7	R	2017-JD4	0	S
2016-JD2	100	R	2017-JD5	11.7	DR
2016-JD3	0	S	2017-JD6	0	S
2016-JD4	0	S	2017-JS1	0	S
2016-JD5	0	S	2017-JS2	0	S
2016-QP1	0	S	2017-JS3	0	S
2016-QP2	0	S	2017-JS4	0	S
2016-QP3	0	S	2017-JS5	0	S
2016-SJ1	0	S	2017-JS6	0	S
2016-SJ2	0	S	2017-JS7	0	S
2017-CM1	67.5	R	2017-MH1	0	S
2017-CM2	0	S	2017-PD1	0	S
2017-CM3	12.5	DR	2017-PD2	0	S
2017-CM4	0	S	2017-PD3	0	S
2017-CM5	0	S	2017-PD4	0	S
2017-CM6	0	S	2017-PD5	22.5	R
2017-CM7	0	S	2017-PD6	0	S
2017-CM8	0	S	2017-PD7	0	S
2017-CM9	75.0	R	2017-PD8	0	S
2017-FX1	0	S	2017-PD9	0	S
2017-FX2	0	S	2017-QP1	0	S
2017-FX3	0	S	2017-QP2	0	S
2017-FX4	0	S	2017-QP3	0	S
2017-JD1	0	S
注：^a敏感种群 (S)：存活率<1%；发展中抗性种群 (DR)：存活率在1%~20%之间；抗性种群 (R)：存活率>20%。Note: ^a Susceptible (S) population: Survival rate<1%; development of resistance (DR) population: 1%≤Survival rate≤20%; resistant (R) population: Survival rate>20%.

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表 5 抗性千金子种群中ACCase基因突变形式及突变频率

Table 5. ACCase gene mutation form and mutation ratio of the resistant Leptochloa chinensis populations

种群 Population	突变形式 Mutation form	突变频率 Mutation ratio/%
2016-JD1	TGG-1999-TCG	88.3
2016-JD2	TGG-1999-TCG	96.7
2016-JD5	N^a	0
2016-QP3	N^a	0
2017-CM1	TGG-2027-TGT	61.7
2017-CM3	TGG-2027-TGT	18.3
2017-CM3	TGG-2027-TCG	30.0
2017-CM9	TGG-2027-TCG	86.7
2017-JD2	TGG-1999-TGT	6.7
2017-JD2	TGG-2027-TGC	11.7
2017-JD3	N^a	0
2017-JD4	N^a	0
2017-JD5	TGG-1999-TGT	68.3
2017-JS1	N^a	0
2017-JS2	N^a	0
2017-PD1	TGG-2027-TCG	5.0
2017-PD5	TGG-1999-TGT	20.0
注：^a N表示ACCase基因已知氨基酸突变位点的密码子未发生碱基突变。Note: ^a N indicate that the corresponding codon of all known mutation sites in ACCase are not found any change.

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