• 百种中国杰出学术期刊
  • 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国高校精品科技期刊
  • 中国国际影响力优秀学术期刊
  • 中国科技核心期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

静电自组装法制备阿维菌素纳米囊悬浮剂及其对黄瓜根结线虫病的防治效果

王瑞 张涛 韩志任 金岩 张大侠 李北兴 刘峰

王瑞, 张涛, 韩志任, 金岩, 张大侠, 李北兴, 刘峰. 静电自组装法制备阿维菌素纳米囊悬浮剂及其对黄瓜根结线虫病的防治效果[J]. 农药学学报, 2022, 24(5): 1215-1223. doi: 10.16801/j.issn.1008-7303.2022.0018
引用本文: 王瑞, 张涛, 韩志任, 金岩, 张大侠, 李北兴, 刘峰. 静电自组装法制备阿维菌素纳米囊悬浮剂及其对黄瓜根结线虫病的防治效果[J]. 农药学学报, 2022, 24(5): 1215-1223. doi: 10.16801/j.issn.1008-7303.2022.0018
WANG Rui, ZHANG Tao, HAN Zhiren, JIN Yan, ZHANG Daxia, LI Beixing, LIU Feng. Preparation of abamectin nanocapsule suspension concentrate by electrostatic self-assembly and its control efficacy on cucumber root-knot nematode[J]. Chinese Journal of Pesticide Science, 2022, 24(5): 1215-1223. doi: 10.16801/j.issn.1008-7303.2022.0018
Citation: WANG Rui, ZHANG Tao, HAN Zhiren, JIN Yan, ZHANG Daxia, LI Beixing, LIU Feng. Preparation of abamectin nanocapsule suspension concentrate by electrostatic self-assembly and its control efficacy on cucumber root-knot nematode[J]. Chinese Journal of Pesticide Science, 2022, 24(5): 1215-1223. doi: 10.16801/j.issn.1008-7303.2022.0018

静电自组装法制备阿维菌素纳米囊悬浮剂及其对黄瓜根结线虫病的防治效果

doi: 10.16801/j.issn.1008-7303.2022.0018
基金项目: 山东省蔬菜产业技术体系 (SDAIT-05).
详细信息
    作者简介:

    王瑞,1220871174@qq.com

    通讯作者:

    刘峰,fliu@sdau.edu.cn

  • 中图分类号: TQ450.6

Preparation of abamectin nanocapsule suspension concentrate by electrostatic self-assembly and its control efficacy on cucumber root-knot nematode

Funds: Technical System of Vegetable Industry in Shandong Province (SDAIT-05).
  • 摘要: 为提高阿维菌素在土壤中的移动扩散能力,改善其对作物根结线虫病的防治效果,采用静电自组装法制备了3%阿维菌素纳米囊悬浮剂,测定了其贮存稳定性和释放性能,并通过土壤淋溶试验与土壤薄层层析试验评价了其在土壤中的移动性,验证了其对南方根结线虫Meloidogyne incognita 2龄幼虫 (J2) 的室内毒力和田间防治效果。结果显示:阿维菌素纳米囊为球形,平均粒径 (D50) 为276.81 nm,在释放液中,60 min时纳米囊悬浮剂中阿维菌素的累积释放率为57.59%,而对照乳油制剂中阿维菌素的累积释放率为94.53%。阿维菌素纳米囊悬浮剂在土壤中横向和纵向的移动距离均明显大于阿维菌素乳油,二者对根结线虫J2在24 h时的LC50值分别为0.257与0.427 mg/L。田间试验中,相同用量下分别采用漫灌法与混土法两种方式施药,药后60 d,3%阿维菌素纳米囊悬浮剂处理比3%阿维菌素乳油处理的防治效果分别高21.82%与4.81%;而药后100 d,前者比后者分别高28.98%与19.79%。表明将阿维菌素制剂纳米化可以增强其在土壤中的移动能力,提高其对黄瓜根结线虫病的防治效果。本研究结果可为开发适合于土壤施用的纳米化农药制剂提供依据。
  • 图  1  阿维菌素纳米囊的扫描电镜图 (A) 与粒径分布图 (B)

    Figure  1.  SEM (A) and particle size distribution (B) of abamectin nanocapsules

    图  2  TGA谱图 (A) 和DSC谱图 (B)

    a. 囊壳材料;b. 阿维菌素原药;c. 阿维菌素纳米囊。

    Figure  2.  The spectrograms of TGA (A) and DSC (B)

    a: shell material; b: abamectin technical material; c: abamectin nanocapsules.

    图  3  纳米囊红外光谱图

    Figure  3.  FTIR spectrogram of nanocapsules

    图  4  不同制剂中阿维菌素的累积释放曲线

    Figure  4.  Accumulative release curves of abamectin from the different formulations

    图  5  不同阿维菌素制剂的土壤淋溶分布 (A,B) 与土壤薄层分布 (C,D)

    Figure  5.  Distribution of different abamectin formulations in soil columns (A, B) and soil layers (C, D)

    图  6  不同阿维菌素制剂对南方根结线虫2龄幼虫24 h致死率

    Figure  6.  Mortality (24 h) of M. incognita J2 treated with different abamectin formulations

    图  7  不同阿维菌素制剂处理的黄瓜根系发病情况

    Figure  7.  The symptoms in cucumber roots caused by M. incognita treated with different abamectin formulations

    图  8  不同阿维菌素制剂处理防治黄瓜根结线虫病效果

    同组处理后不同字母表示应用Tukey检验在 0.05 水平上差异显著。

    Figure  8.  Control efficacy of different abamectin formulations on cucumber root-knot nematode

    The columns in the same group followed by different letters indicated significantly differences at the 0.05 level according to Tukey HSD.

    表  1  3%阿维菌素纳米囊悬浮剂冷热贮稳定性

    Table  1.   Stability of abamectin 3% nanocapsules suspension after cold and hot storages

    项目
    Item
    pH 值
    pH value
    平均粒径
    Particle size
    总有效成分含量
    Total content of
    a.i./%
    D50/nmD90/nm
    初始制剂
    Initial formulation
    7.5 276.81 480.87 3.00
    热贮
    Hot storage
    7.3 265.33 500.56 2.99
    冷贮
    Cold storage
    7.2 255.36 457.33 2.98
    下载: 导出CSV
  • [1] 席先梅, 白全江, 李玉民, 等. 41.7% 氟吡菌酰胺悬浮剂不同施药时期对黄瓜根结线虫的防治效果[J]. 植物保护, 2021, 47(5): 314-319.

    XI X M, BAI Q J, LI Y M, et al. Control effect of 41.7% fluopyram suspension on cucumber root-knot nematode at different application stages[J]. Plant Prot, 2021, 47(5): 314-319.
    [2] 任玉鹏. 滴灌施用阿维菌素防治番茄根结线虫病的可行性评价[D]. 泰安: 山东农业大学, 2016.

    REN Y P. Feasibility evaluation of applying abamectin by drip irrigation for control Meloidogyne incognita disease[D]. Taian: Shandong Agricultural University, 2016.
    [3] 李浩林. 腐植酸盐作为阿维菌素水悬浮剂助剂的可行性研究[D]. 泰安: 山东农业大学, 2021.

    LI H L. Feasibility study on humic acid salt as a dispersant of abamectin suspension[D]. Taian: Shandong Agricultural University, 2021.
    [4] JING T F, ZHANG D X, PAN S H, et al. Phenyl isocyanate-modified avermectin B1a improves the efficacy against plant-parasitic nematode diseases by facilitating its soil mobility[J]. ACS Sustain Chem Eng, 2020, 8(5): 2310-2319. doi: 10.1021/acssuschemeng.9b07243
    [5] 李素霞, 张杰, 张文娟, 等. 不同加工剂型阿维菌素对南方根结线虫的防治效果[J]. 植物保护学报, 2013, 40(6): 575-576.

    LI S X, ZHANG J, ZHANG W J, et al. The effect of formulations of abamectin against root-knot nematode Meloidogyne incognita[J], J Plant Prot, 2013, 40(6): 575-576.
    [6] 张文娟, 齐浩亮, 任玉鹏, 等. 剂型、灌水量和土壤疏松度对甲维盐在土壤中分布及防治根结线虫效果的影响[J]. 植物保护学报, 2015, 42(3): 440-446. doi: 10.13802/j.cnki.zwbhxb.2015.03.023

    ZHANG W J, QI H L, REN Y P, et al. Effects of formulations, irrigation volumes and loose degree of soils to the distribution of emamectin benzoate in soils on root-knot nematode Meloidogyn spp.[J]. J Plant Prot, 2015, 42(3): 440-446. doi: 10.13802/j.cnki.zwbhxb.2015.03.023
    [7] LI B X, REN Y P, ZHANG D X, et al. Modifying the formulation of abamectin to promote its efficacy on southern root-knot nematode (Meloidogyne incognita) under blending-of-soil and root-irrigation conditions[J]. J Agric Food Chem, 2018, 66(4): 799-805. doi: 10.1021/acs.jafc.7b04146
    [8] CAO J, GUENTHER R H, SIT T L, et al. Development of abamectin loaded plant virus nanoparticles for efficacious plant parasitic nematode control[J]. ACS Appl Mater Inter, 2015, 7(18): 9546-9553. doi: 10.1021/acsami.5b00940
    [9] LI Z Z, CHEN J F, LI Y, et al. Adsorption of avermectin on porous hollow silica nanoparticles by supercritical technology[J]. J Nanosci Nanotechnol, 2007, 7(2): 535-541. doi: 10.1166/jnn.2007.148
    [10] 张艳伟, 慕慧, 赵虹霞, 等. 空心多孔纳米 ZrO2 载体材料载药性能的研究[J]. 西北药学杂志, 2010, 25(6): 443-446. doi: 10.3969/j.issn.1004-2407.2010.06.023

    ZHANG Y W, MU H, ZHAO H X, et al. Study on the properties of novel hollow zirconia nanoparticles carrier[J]. Northwest Pharmacy J, 2010, 25(6): 443-446. doi: 10.3969/j.issn.1004-2407.2010.06.023
    [11] KAZIEM A E, GAO Y H, ZHANG Y, et al. α-Amylase triggered carriers based on cyclodextrin anchored hollow mesoporous silica for enhancing insecticidal activity of avermectin against Plutella xylostella[J]. J Hazard Mater, 2018, 359: 213-221. doi: 10.1016/j.jhazmat.2018.07.059
    [12] LIANG W L, WANG A X, ZHENG G D, et al. Chitosan-based nanoparticles of avermectin to control pine wood nematode[J]. Inter J Biol Macromol, 2018, 112: 258-263. doi: 10.1016/j.ijbiomac.2018.01.174
    [13] ZHANG D X, LIU G, JING T F, et al. Lignin-modified electronegative epoxy resin nanocarriers effectively deliver pesticides against plant root-knot nematode (Meloidogyne incognita)[J]. J Agric Food Chem, 2020, 68(47): 13562-13572. doi: 10.1021/acs.jafc.0c01736
    [14] ZHANG D X, DU J, WANG R, et al. Core/shell dual-responsive nanocarriers via iron-mineralized electrostatic self-assembly for precise pesticide delivery[J]. Adv Funct Mater, 2021, 31(34): 2102027. doi: 10.1002/adfm.202102027
    [15] 农药热贮稳定性测定方法: GB/T 19136—2003[S]. 北京: 中国标准出版社, 2003.

    Testing method for the storage stability at elevated temperature of pesticides: GB/T 19136—2003[S]. Beijing: Standards Press of China, 2003.
    [16] 农药低温稳定性测定方法: GB/T 19137—2003[S]. 北京: 中国标准出版社, 2003.

    Testing method for the stroage stability at low temperature of pesticides: GB/T 19137—2003[S]. Beijing: Standards Press of China, 2003.
    [17] MT189. Determination of free lambda-cyhalothrin in CS formulations[M]//CIPAC Handbook. Physio-chemical methods for technical formulated pesticides. Oxfordshire: Marston Book Services Ltd. , 2004: 137-139.
    [18] MT190. Determination of release properties of lambda-cyhalothrin CS formulations[M]//CIPAC Handbook. Physio-chemical methods for technical formulated pesticides. Oxfordshire: Marston Book Services Ltd. , 2004: 140-142.
    [19] 化学农药环境安全评价试验准则第 5 部分: 土壤淋溶试验: GB/T 31270.5—2014[S]. 北京: 中国标准出版社, 2015.

    Test guidelines on environmental safety assessment for chemical pesticides. Part 5, soil leaching test: GB/T 31270.5— 2014[S]. Beijing: Standards Press of China, 2015.
    [20] 莫明和, 桑维均, 张克勤, 等. 分布于南方的北方根结线虫初报[J]. 西南农业学报, 1997(S1): 101-104. doi: 10.16213/j.cnki.scjas.1997.s1.019

    MO M H, SANG W J, ZHANG K Q, et al. A preliminary report on northern root-knot nematode distributed in South China[J]. Southwest J Agric Sci, 1997(S1): 101-104. doi: 10.16213/j.cnki.scjas.1997.s1.019
    [21] NEL A, XIA T, MÄDLER L, et al. Toxic potential of materials at thenanolevel[J]. Science, 2006, 311(5761): 622-627. doi: 10.1126/science.1114397
    [22] 孙长娇, 王琰, 赵翔, 等. 纳米农药剂型与其减施增效机理研究进展[J]. 农药学学报, 2020, 22(2): 205-213. doi: 10.16801/j.issn.1008-7303.2020.0058

    SUN C J, WANG Y, ZHAO X, et al. Progress on categories and synergistic mechanism of nanopesticides[J]. Chin J Pestic Sci, 2020, 22(2): 205-213. doi: 10.16801/j.issn.1008-7303.2020.0058
    [23] KHANDELWAL N, BARBOLE R S, BANERJEE S S, et al. Budding trends in integrated pest management using advanced microandnano-materials: challenges and perspectives[J]. J Environ Manag, 2016, 184: 157-169. doi: 10.1016/j.jenvman.2016.09.071
    [24] 刘广. 阿维菌素纳米囊的制备及对黄瓜根结线虫病防治作用[D]. 泰安: 山东农业大学, 2020.

    LIU G. Preparation of abamectin nanocapsuless and its against root-knot nematode (Meloidogyne incognita) disease[D]. Taian: Shandong Agricultural University, 2020.
    [25] GRUBER V F, HALLEY B A, CHEN S H, et al. Mobility of avermectin B1a in soil[J]. J Agric Food Chem, 1990, 38(3): 886-890. doi: 10.1021/jf00093a063
    [26] 张卫. 农药阿维菌素在环境中降解和代谢研究[D]. 杭州: 浙江大学, 2004.

    ZHANG W. Degradation and metabolism of abamectin in environment[D]. Hangzhou: Zhejiang University, 2004.
  • 加载中
图(8) / 表(1)
计量
  • 文章访问数:  56
  • HTML全文浏览量:  24
  • PDF下载量:  20
  • 被引次数: 0
出版历程
  • 收稿日期:  2022-01-25
  • 录用日期:  2022-02-19
  • 网络出版日期:  2022-03-04
  • 刊出日期:  2022-10-10

目录

    /

    返回文章
    返回