昆虫信息化合物的筛选及鉴定技术研究进展

钱沉鱼; 苑鹏宇; 马涛; 温秀军

doi:10.16801/j.issn.1008-7303.2022.0068

昆虫信息化合物的筛选及鉴定技术研究进展

doi: 10.16801/j.issn.1008-7303.2022.0068

1.
华南农业大学林学与风景园林学院，广东省森林植物种质创新与利用重点实验室，广州 510642

基金项目: 中央财政林业科技推广示范项目 (2022GDTK-06)；国家自然科学基金项目 (31600516).

详细信息

作者简介:
钱沉鱼，qianchenyu94@163.com

通讯作者:
温秀军，wenxiujun@scau.edu.cn

中图分类号: TQ453.8
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出版历程
- 收稿日期: 2022-02-11
- 录用日期: 2022-06-09
- 网络出版日期: 2022-07-19
- 刊出日期: 2022-12-02

Research progress on screening and identification techniques of insect semiochemicals

1.
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China

Funds: the Central Financial Forestry Science and Technology Extension Project (2022GDTK-06); National Natural Science Foundation of China (31600516).

摘要

摘要: 在化学生态学领域中，触角电位仪 (EAG)、气相色谱-触角电位联用仪 (GC-EAD)、气相色谱-质谱联用仪 (GC-MS)、单感器记录仪 (SSR) 和气相色谱-单感器记录联用仪 (GC-SSR) 是开展昆虫信息化合物的鉴定及活性组分筛选的重要技术手段，常用于昆虫对信息化合物感受机制、昆虫与植物的互作关系研究。本文详细分析了这几种技术的作用机理，综述了近些年昆虫电生理技术的应用和研究进展，可为昆虫信息化合物的研究提供技术参考。
- 触角电位仪 /
- 气相色谱-触角电位联用仪 /
- 气相色谱-质谱联用仪 /
- 单感器记录仪 /
- 气相色谱-单感器记录联用仪
Abstract: In the chemical ecology field, electroantennography (EAG), gas chromatography-electroantennographic detection (GC-EAD), gas chromatography-mass spectrometer (GC-MS), single sensillum recordings (SSR), and gas chromatography-single sensillum recordings (GC-SSR) play significant roles in the identification, screening of insect infochemicals and are widely used in chemoreception mechanism of semiochemical, and the investigation of the interactions between insects and plants. Here, the functions and mechanisms of the five above-mentioned technologies were expounded, their applications were summarized, and will provide technical references for the research of insect semiochemicals from the perspective of chemecology.

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图 1 EAG仪器原理示意图^[32]

Figure 1. Schematic diagram of EAG equipment^[32]

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图 2 GC-EAD仪器操作原理图

Figure 2. Schematic diagram of GC-EAD equipment

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图 3 地中海果蝇雄性信息素的GC × GC-TOFMS分析及EAD响应^[101]

Figure 3. The GC × GC-TOFMS analysis and GC-EADs response of C. capitata male pheromone^[101]

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表 1 适用于不同信息素类型的GC-EAD条件 (2012—2022)

Table 1. The application of GC-EAD on pheromones (2012-2022)

类型 Type	测试昆虫　　 Analyte	化合物类型　　 Compound	色谱柱 GC column	参考文献 Reference
性信息素 Sex pheromone	三点盲蝽 Adelphocoris fasciaticollis	酯类和醛类 Ester and aldehyde	HP-5	[52]
	朱红毛斑蛾 Phauda flammans	醛类 Aldehyde	HP-5	[53]
	杨小舟蛾 Micromelalopha siversi	醛类 Aldehyde	HP-5	[54]
	草地贪夜蛾 Spodoptera frugiperda	酸酯类 Acid ester	HP-5	[55]
	三角凹缘天蛾 Neogurelca himachala sangaica	醛类 Aldehyde	HP-5MS	[56]
	潜叶蛾 Holocacista capensis	醛类 Aldehyde	HP-INNOWax	[57]
	团花绢野螟 Diaphania glauculalis	醛类 Aldehyde	DB-5	[45]
	灰茶尺蠖 Ectropis grisescens	烯烃 Alkene	DB-5	[43]
	绿盲蝽 Apolygus lucorum	酯类和醛类 Ester and aldehyde	DB-5	[58]
	叩头甲和Limonius californicus Limonius canus and Limonius californicus	酸类 Acid	DB-5	[59]
	西印度按实蝇 Anastrepha obliqua	萜类 Terpene	DB-5	[60]
	绿翅绢野螟 Diaphania angustalis	醛类和醇类 Aldehyde and alcohol	DB-5MS	[47]
	女贞尺蠖 Naxa seriaria	烯烃 Alkene	DB-5MS	[61]
	橡树叶蛾 Tischeria ekebladella	烯烃 Alkene	DB-Wax	[62]
	苜蓿盲蝽 Adelphocoris lineolatus	酯类和醛类 Ester and aldehyde	DB-Wax	[52]
	牧草盲蝽 Lygus pratensis	酯类和醛类 Ester and aldehyde	DB-Wax	[63]
	棉铃虫 Helicoverpa armigera	醛类 Aldehyde	DB-Wax and HP-88	[64]
	鸢尾蛾 Monochroa divisella	酯类 Ester	DB-23	[65]
	Anthistarcha binocularis	萜类 Terpene	Rtx-5	[66]
	Trissolcus brochymenae	酯类和醛类 Ester and aldehyde	Varian CP8945	[67]
	荨麻毛虫 Monema flavescens	醛类和醇类 Aldehyde and alcohol	DB-23 and TG-5 MS	[68]
	橄榄果蝇 Bactrocera oleae	烯烃 Alkene	SPB-5	[69]
	桃红颈天牛 Aromia bungii	醛类 Aldehyde	CP-ChiraSil-DEX CB	[70]
	Cydia fagiglandana 和 Cydia splendana Cydia fagiglandana and Cydia splendana	酯类和醇类 Aldehyde and alcohol	SUPELCO Equity-5	[71]
	果蝇 Bactrocera kraussi	酯类 Ester	SH-Rtx-5Sil MS	[72]
聚集信息素 Aggregation pheromone	豆花薊馬 Megalurothrips usitatus	酯类 Ester	HP-5	[73]
聚集信息素 Aggregation pheromone	落叶松八齿小蠹 Ips subelongatus	萜类 Terpene	CycloSil-B	[74]
告警信息素 Alarm pheromone	墨胸胡蜂 Vespa velutina	酮类和醇类 Ketone and alcohol	HP-5	[75]

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表 2 适用于不同挥发物类型的GC-EAD条件 (2012—2022年)

Table 2. The application of GC-EAD on volatiles (2012-2022)

类型 Type	样品　　　　 Sample	测试昆虫　　 Analyte	化合物类型　　　 Compound	色谱柱 GC column	参考文献 Reference
植物源挥发物 Plant volatiles	玉米植株 Zea mays plant	玉米禾螟 Chilo partellus	萜类 Terpene	HP-1	[76]
	野葛和大豆 Pueratia montana and Glycine max	筛豆龟蝽 Megacopta cribraria	萜类 Terpene	HP-5	[77]
	肉桂、薰衣草和桉树精油 Cinnamomum cassia, Lavandula angustifolia and Eucalyptus robusta essential oil	盒子树蛾 Cydalima perspectalis	萜类 Terpene	HP-5	[78]
	茶 Camellia sinensis	茶黑毒蛾 Dasychira baibarana	酯类、醇类和酮类 Ester, alcohol and ketone	HP-5	[79]
	槐树 Sophora japonica	异色瓢虫 Harmonia axyridis	醛类 Aldehyde	HP-5MS	[80]
	欧洲云杉 Picea abies	云杉八齿小蠹 Ips typographus	芳香烃、萜类 Aromatic hydrocarbon, terpene	HP-5MS	[81]
	蛇床花 Cnidium monnieri	异色瓢虫 Harmonia axyridis	芳香烃 Aromatic hydrocarbon	HP-5MS	[82]
	红枫、黑樱桃和南方红橡木等 Acer rubrum, Prunus serotina and Quercus falcata, etc.	南部松小蠹 Dendroctonus frontalis	醇类、醛类和酸 Alcohol, aldehyde, and acid	HP-INNOWax	[83]
	迷迭香 Rosmarinus officinalis	茶尺蠖 Ectropis obliqua	萜类 Terpene	DB-5	[84]
	烟草 Nicotiana tabacum	烟青虫 Helicoverpa assulta	醛类 Aldehyde	DB-5	[85]
	向日葵 Helianthus annuus	棕纹蝽 Halyomorpha halys	萜类 Terpene	DB-5	[86]
	小果咖啡和中果咖啡 Coffea arabica and Coffea canephora	白茎螟 Xylotrechus quadripes	萜类 Terpene	DB-5MS	[87]
	豌豆 Pisum sativum	豆荚小卷蛾 Cydia nigricana	萜类 Terpene	DB-Wax	[88]
	豇豆和扁豆的花 Flower of Vigna unguiculata and Lablab purpureus	豆荚螟 Maruca vitrata	萜类 Terpene	DB-Wax	[89]
	蓖麻 Ricinus communis	红颈常室茧蜂和绿盲蝽 Peristenus spretus and Apolygus lucorum	烃类和酮类 Hydrocarbon and ketone	DB-Wax	[90]
	杏树 Armeniaca spp.	脐腹小蠹 Scolytus schevyrewi	萜类 Terpene	DB-Wax	[91]
	花生植株 Arachis hypogaea plant	暗黑鳃金龟 Holotrichia parallela	萜类和醛类 Terpene and aldehyde	DB-Wax	[92]
	沙针 Osyris wightiana	斑翅果蝇 Drosophila suzukii	萜类、醇类和酯类 Terpene, alcohol and ester	DB-Wax	[93]
	迪萨兰 Disa forficaria	天牛 Chorothyse hessei	酯类和酸类 Ester and acid	ZB-SemiVolatiles	[94]
	番石榴或甜橙 Psidium guajava or Citrus sinensis	按实蝇 Anastrepha striata	酯类和醇类 Ester and alcohol	VF-5 MS	[95]
	Periploca laevigata	家蝇 Musca domestica	萜类和硫化物 Terpene and sulfide	ZB-5	[96]
	欧洲云杉 Picea abies	云杉球果小卷蛾Cydia strobilella	单萜 Monoterpene	HP-INNOWax	[97]
表皮挥发物 Cuticular hydrocarbon	美国白蛾、柞蚕和舞毒蛾的蛹 Pupa of Hyphantria cunea, Antheraea pernyi and Lymantria dispar	白蛾周氏啮小蜂 Chouioia cunea	烷烃 Alkane	HP-5MS	[98]
	番荔枝实蝇、纳塔尔实蝇和 Ceratitis fasciventri Ceratitis anonae, Ceratitis rosa and Ceratitis fasciventri		酯类、萜类和酮类 Ester, terpene and ketone	Rxi-5Sil MS	[99]
	截尾丽虎天牛 Plagionotus detritus		酮类 Ketone	HP-5	[100]
	地中海果蝇 Ceratitis capitata		酯类、萜类和酮类 Ester, terpene and ketone	Rxi-5Sil MS	[101]

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