• 百种中国杰出学术期刊
  • 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国高校精品科技期刊
  • 中国国际影响力优秀学术期刊
  • 中国科技核心期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

30亿CFU/g芽胞杆菌可湿性粉剂的研制及其对马铃薯黄萎病和疮痂病的防治效果

张晓云 丛蓉 赵卫松 曲远航 苏振贺 郭庆港 鹿秀云 李社增 马平

张晓云, 丛蓉, 赵卫松, 曲远航, 苏振贺, 郭庆港, 鹿秀云, 李社增, 马平. 30亿CFU/g芽胞杆菌可湿性粉剂的研制及其对马铃薯黄萎病和疮痂病的防治效果[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0081
引用本文: 张晓云, 丛蓉, 赵卫松, 曲远航, 苏振贺, 郭庆港, 鹿秀云, 李社增, 马平. 30亿CFU/g芽胞杆菌可湿性粉剂的研制及其对马铃薯黄萎病和疮痂病的防治效果[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0081
ZHANG Xiaoyun, CONG Rong, ZHAO Weisong, QU Yuanhang, SU Zhenhe, GUO Qinggang, LU Xiuyun, LI Shezeng, MA Ping. Development of 3 billion CFU/g Bacillus wettable powder and its control efficacy on potato Verticillium wilt and scab[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0081
Citation: ZHANG Xiaoyun, CONG Rong, ZHAO Weisong, QU Yuanhang, SU Zhenhe, GUO Qinggang, LU Xiuyun, LI Shezeng, MA Ping. Development of 3 billion CFU/g Bacillus wettable powder and its control efficacy on potato Verticillium wilt and scab[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0081

30亿CFU/g芽胞杆菌可湿性粉剂的研制及其对马铃薯黄萎病和疮痂病的防治效果

doi: 10.16801/j.issn.1008-7303.2022.0081
基金项目: 河北省重点研发计划 (20326515D);河北省农林科学院科技创新专项 (2022KJCXZX-ZBS-1)
详细信息
    作者简介:

    张晓云,zxy_zxl@163.com

    通讯作者:

    李社增,shezengli@163.com

  • 中图分类号: S482.7;TQ450.6;TQ458

Development of 3 billion CFU/g Bacillus wettable powder and its control efficacy on potato Verticillium wilt and scab

Funds: the Key R & D Program of Hebei Province(20326515D);Science and Technology Innovation Project of Hebei Academy of Agriculture and Forestry(2022KJCXZX-ZBS-1)
  • 摘要: 黄萎病和疮痂病是马铃薯生产上的重要土传病害,严重影响马铃薯的产量与品质,但缺乏有效的防治药剂。本研究以枯草芽胞杆菌HMB26553和解淀粉芽胞杆菌PHODG36为有效成分,通过对润湿剂、分散剂及紫外保护剂的筛选,确定了30亿CFU/g芽胞杆菌可湿性粉剂的配方(质量分数):HMB26553菌株母药10%、PHODG36菌株母药10%、润湿剂LT-569 1%、分散剂MF 2%、紫外保护剂抗坏血酸1%,滑石粉76%。该制剂芽胞数量37.6亿 CFU/g,杂菌率2.8%、pH 7.8、细度98.2%、干燥减量1.6%、润湿时间95.7 s、悬浮率80.7%。温室盆栽试验结果表明:该可湿性粉剂1~3 kg/667 m2拌种处理对马铃薯出苗安全,对生长无不良影响,对马铃薯黄萎病的防效为45.1%~47.4%。田间小区试验结果表明:该可湿性粉剂1~3 kg/667 m2拌种处理均能显著减轻马铃薯疮痂病的发生,其中2 kg/667 m2拌种处理防效高达72.0%,增产率达15.5%。不同地区的田间示范应用结果表明:该可湿性粉剂2 kg/667 m2拌种处理对马铃薯黄萎病的防治效果为50.8%~62.1%,疮痂病为63.9%~65.7%,增产率为14.3%~29.4%。本研究结果表明,30亿CFU/g芽胞杆菌可湿性粉剂能有效防治马铃薯土传病害且增产作用明显,为该制剂在马铃薯生产上应用提供了科学依据。
  • 图  1  紫外保护剂对芽胞杆菌可湿性粉剂芽胞存活率的影响

    注:图中数据为平均值±标准误。不同小写字母表示经Duncan氏新复极差法检验在0.05水平上差异显著。

    Figure  1.  Effects of UV-protective agents on spore survival rate of Bacillus WP

    Note: The data were mean± SE. The different lowercase letters indicated significant difference at 0.05 level by Duncan’s new multiple range test.

    图  2  30亿CFU/g芽胞杆菌可湿性粉剂不同用量对马铃薯出苗及株高的影响

    注:图中数据为平均值±标准误。不同小写字母表示经Duncan氏新复极差法检验在0.05水平上差异显著。

    Figure  2.  Effects of different doses of 3 billion CFU/g Bacillus WP on potato emergence and plant growth

    Note: The data were shown as mean± SE. The different lowercase indicated significant difference at 0.05 level by Duncan’s new multiple range test.

    图  3  盆栽试验中30亿CFU/g芽胞杆菌可湿性粉剂对马铃薯黄萎病的防治效果

    注:图中数据为平均值±标准误。不同小写字母表示经Duncan氏新复极差法检验在0.05水平上差异显著。

    Figure  3.  Control efficacy of 3 billion CFU/g Bacillus WP on potato verticillium wilt in pot test

    Note: The data were shown as mean± SE. The different lowercase indicated significant difference at 0.05 level by Duncan’s new multiple range test.

    表  1  供试助剂信息表

    Table  1.   The information of tested adjuvants

    助剂
    Adjuvant
    用途
    Usage
    生产厂家
    Manufacturer
    助剂
    Adjuvant
    用途
    Usage
    生产厂家
    Manufacturer
    十二烷基苯磺酸钠
    Sodium dodecyl benzene sulfonate (SDBS)
    润湿剂
    Wetting agent
    山东隆汇化工有限公司
    Shandong Longhui Chemical Co., Ltd.
    亚甲基双萘磺酸钠
    Sodium salt of polynaphthalene sulphonic acid (NNO)
    分散剂
    Dispersant
    山东优索化工科技有限公司
    Shandong Yousuo Chemical Technology Co., Ltd.
    拉开粉
    BXButyl naphthalene-1-sulfonate, sodium
    润湿剂
    Wetting agent
    绍兴浙创化工有限公司
    Shaoxing Zhechuang Chemical Co., Ltd.
    聚萘甲醛磺酸钠盐
    Sodium poly[(naphthaleneformaldehyde)sulfonate] (MF)
    分散剂
    Dispersant
    山东优索化工科技有限公司
    Shandong Yousuo Chemical Technology Co., Ltd.
    Berol 790A 润湿剂
    Wetting agent
    南京捷润科技有限公司
    Nanjing JieRun Sci. Tech. Co., Ltd.
    Morwet D-425 分散剂
    Dispersant
    南京捷润科技有限公司
    Nanjing JieRun Sci. Tech. Co., Ltd.
    SP-2846BW 润湿剂
    Wetting agent
    江苏擎宇化工科技有限公司
    Jiangsu SinvoChem S&T Co., Ltd.
    荧光素钠
    Fluorescein sodium
    紫外保护剂
    UV-protective agent
    天津拉斯维特化工贸易有限公司
    Tianjin Lasiweite Chemical Trade Co., Ltd.
    GY-W04 润湿剂
    Wetting agent
    北京广源益农化学有限责任公司
    Beijing Grand AgroChem Co., Ltd.
    糊精
    Dextrin
    紫外保护剂
    UV-protective agent
    上海源叶生物科技有限公司
    Shanghai Yuanye Biotechnology Co., Ltd.
    LT-569 润湿剂
    Wetting agent
    深圳市朗钛生物科技有限公司
    Shenzhen Langtai Biotechnology Co., Ltd.
    抗坏血酸
    Ascorbic acid
    紫外保护剂
    UV-protective agent
    山东德彦化工有限公司
    Shandong Deyan Chemical Co., Ltd.
    下载: 导出CSV

    表  2  不同助剂与HMB26553菌株和PHODG36菌株的生物相容性

    Table  2.   Bio-compatibility evaluation between different adjuvants and B. subtilis HMB26553 or B. amyloliquefaciens PHODG36

    助剂 AdjuvantHMB26553 PHODG36
    1%2%3% 1%2%3%
    润湿剂 Wetting agent LT-569 + + + + + +
    GY-W04
    BX
    SDBS
    SP2846
    790A
    分散剂 Dispersant D-425
    NNO + + ± + + ±
    MF + + ± + + ±
    紫外保护剂 UV-protective agent 荧光素钠 Fluorescein sodium
    糊精 Dextrin + + + + + +
    抗坏血酸Ascorbic acid + + + + + +
    注:“+”表示供试芽胞杆菌菌株生长正常;“−”表示供试芽胞杆菌生长受到完全抑制;“±”表示供试芽胞杆菌生长受到部分抑制。Note: “+” indicates the tested Bacillus isolate grows normally, “−” indicates the growth of the tested Bacillus isolate is completely inhibited, and “±” indicates the growth of the tested Bacillus isolate is partially inhibited.
    下载: 导出CSV

    表  3  润湿剂及分散剂最佳用量筛选

    Table  3.   Optimal dosage screening of wetting and dispersing agent

    编号
    Number
    LT-569
    (%, W/W)
    NNO
    (%, W/W)
    MF
    (%, W/W)
    润湿时间
    Wetting time/s
    悬浮率
    Suspension rate/%
    1110112.3 ± 1.3 c80.4 ± 1.6 ab
    2120105.3 ± 2.0 d82.5 ± 0.8 a
    310199.7 ± 1.8 e81.3 ± 1.6 ab
    410293.7 ± 1.5 f80.5 ± 1.3 ab
    5210128.0 ± 2.3 b77.3 ± 1.2 bc
    6220123.0 ± 1.2b76.5 ± 1.7 cd
    7201113.0 ± 1.2 c74.7 ± 1.5 cde
    820295.3 ± 1.9 ef74.5 ± 1.6 cde
    9310128.0 ± 2.1 b72.6 ± 1.0 de
    10320144.3 ± 1.2 a71.1 ± 0.6 e
    11301123.0 ± 2.5 b74.4 ± 0.9 cde
    1230295.0 ± 0.6 ef72.5 ± 0.7 de
    注:表中数据为平均值 ± 标准误,同列数据后不同小写字母表示经Duncan氏新复极差法检验在0.05水平上差异显著。Note: The data were mean ± SE. The different lowercase letters in the same column indicated significant difference at 0.05 level by Duncan’s new multiple range test.
    下载: 导出CSV

    表  4  芽胞杆菌可湿性粉剂主要指标检测结果

    Table  4.   The main indexes of Bacillus WP

    指标
    Index
    国家标准值
    National standards
    实际测定值
    Actual value
    芽胞量 Spore content/(亿CFU/g) 37.6 ± 0.6
    杂菌率 Rate of mixed bacteria/% ≤3 2.8 ± 0.0
    pH值 pH value 5.0-8.0 7.8 ± 0.0
    细度 Fineness (≤44 μm)/% ≥90 98.2 ± 0.4
    干燥减量 Loss on drying/% ≤6 1.6 ± 0.2
    润湿时间 Wetting time/s ≤180 95.7 ± 0.9
    悬浮率 Suspension rate/% ≥75 80.7 ± 0.8
    注:表中数据为平均值 ± 标准误。Note: The data were shown as mean ± SE.
    下载: 导出CSV

    表  5  田间小区试验中30亿CFU/g芽胞杆菌可湿性粉剂对马铃薯疮痂病及产量的影响

    Table  5.   The effect of 3 billion CFU/g Bacillus WP on the occurrence of potato scab and the potato yield in field plot trial

    处理
    Treatment
    施药剂量
    Dosage (kg/667 m2)
    发病率
    Incidence/%
    防治效果
    Control efficacy/%
    产量
    Yield/(kg/667 m2)
    增产率
    Yield-increase rate /%
    空白对照 CK 3 26.5 ± 1.0a 3431.8 ± 93.0 b
    化学药剂 Chemical 3 11.1 ± 1.8 b 56.8 3908.8 ± 169.4 ab 13.9
    芽胞杆菌可湿性粉剂 Bacillus WP 1 12.7 ± 0.5 b 50.6 3797.6 ± 74.9 ab 10.7
    2 7.2 ± 0.6 c 72.0 3964.4 ± 265.0 a 15.5
    3 12.2 ± 0.6 b 52.5 3640.2 ± 61.2 ab 6.1
    注:表中数据为平均值 ± 标准误,同列数据后不同小写字母表示经Duncan氏新复极差法检验在0.05水平上差异显著。化学药剂为70%甲基托布津可湿性粉剂0.1 kg、3%中生菌素可湿性粉剂0.1 kg与滑石粉2.8 kg混匀。Note: The data were shown as mean ± SE. The different lowercase in the same column indicated significant difference at 0.05 level by Duncan’s new multiple range test. Chemical is the mixture of 70% thiophanate-methyl WP (0.1 kg), 3% zhongshengmycin WP (0.1 kg) and talcum powder (2.8 kg).
    下载: 导出CSV

    表  6  田间示范试验中30亿CFU/g芽胞杆菌可湿性粉剂对马铃薯黄萎病及产量的影响

    Table  6.   The effects of 3 billion CFU/g Bacillus WP on the occurrence of potato verticillium wilt and the potato yield in large-scale field demonstration

    地点   
    Location   
    处理   
    Treatment   
    病情指数
    Disease
    index
    防治效果
    Control
    efficacy/%
    产量
    Yield/(kg/667 m2)
    增产率
    Yield-increase
    rate/%
    桃山村 Taoshan village 化学药剂 Chemical 7.8 ± 0.9 a 3525.4 ± 202.3 b
    芽胞杆菌可湿性粉剂Bacillus WP 3.0 ± 0.1 b 62.1 4514.7 ± 145.6 a 28.1
    拔上村 Bashang village 化学药剂 Chemical 15.1 ± 2.7 a 2433.1 ± 142.8 a
    芽胞杆菌可湿性粉剂 Bacillus WP 7.6 ± 0.7 b 50.8 2781.4 ± 169.8 a 14.3
    注:表中数据为平均值 ± 标准误,同列数据后不同小写字母表示经独立样本t检验在0.05 水平上差异显著。化学药剂为70%甲基托布津可湿性粉剂0.1 kg、3%中生菌素可湿性粉剂0.1 kg与滑石粉2.8 kg混匀。Note: The data were shown as mean ± SE, the different lowercase in the same column indicated significant difference at 0.05 level by the independent sample t-test. Chemical is the mixture of 70% thiophanate-methyl WP (0.1 kg), 3% zhongshengmycin WP (0.1 kg) and talcum powder (2.8 kg).
    下载: 导出CSV

    表  7  田间示范试验中30亿CFU/g芽胞杆菌可湿性粉剂对马铃薯疮痂病和产量的影响

    Table  7.   The effects of 3 billion CFU/g Bacillus WP on the occurrence of potato scab and the potato yield in large-scale field demonstration

    地点
    Location
    处理
    Treatment
    病情指数
    Disease
    index
    防治效果
    Control
    efficacy/%
    产量
    Yield/(kg/667 m2)
    增产率
    Yield-increase
    rate/%
    十九号村 Shijiuhao village 化学药剂 Chemical 16.9 ± 1.8 a 2815.8 ± 210.7 b
    芽胞杆菌可湿性粉剂Bacillus WP 6.1 ± 1.7 b 63.9 3643.3 ± 130.7 a 29.4
    桦树林村 Huashulin village 化学药剂 Chemical 10.9 ± 1.5 a 3848.3 ± 110.1 b
    芽胞杆菌可湿性粉剂Bacillus WP 3.8 ± 0.7 b 65.7 4488.0 ± 84.3 a 16.7
    注:表中数据为平均值 ± 标准误,同列数据后不同小写字母表示经独立样本t检验在0.05 水平上差异显著。化学药剂为70%甲基托布津可湿性粉剂0.1 kg、3%中生菌素可湿性粉剂0.1 kg与滑石粉2.8 kg混匀。Note: The data were shown as mean ± SE, the different lowercase in the same column indicated significant difference at 0.05 level by the independent sample t-test. Chemical is a mixture of 70% thiophanate-methyl WP (0.1 kg), 3% zhongshengmycin WP (0.1 kg) and talcum powder (2.8 kg).
    下载: 导出CSV
  • [1] 徐进, 朱杰华, 杨艳丽, 等. 中国马铃薯病虫害发生情况与农药使用现状[J]. 中国农业科学, 2019, 52(16): 2800-2808. doi: 10.3864/j.issn.0578-1752.2019.16.006

    XU J, ZHU J H, YANG Y L, et al. Status of major diseases and insect pests of potato and pesticide usage in China[J]. Sci Agric Sin, 2019, 52(16): 2800-2808. doi: 10.3864/j.issn.0578-1752.2019.16.006
    [2] 王丽丽, 符桂华, 马金贵, 等. 乌昌地区马铃薯黄萎病菌分离与鉴定[J]. 新疆农业科学, 2014, 51(4): 667-672.

    WANG L L, FU G H, MA J G, et al. Isolation and identification of the pathogens causing Verticillium wilt of potato in Urumqi and Changji area, Xinjiang[J]. Xinjiang Agric Sci, 2014, 51(4): 667-672.
    [3] 陈爱昌, 魏周全, 马永强, 等. 甘肃省马铃薯黄萎病病原分离与鉴定[J]. 植物病理学报, 2013, 43(4): 418-420. doi: 10.13926/j.cnki.apps.2013.04.012

    CHEN A C, WEI Z Q, MA Y Q, et al. Isolation and indentification of the pathogens causing Potato Verticillium wilt in Gansu[J]. Acta Phytopathol Sin, 2013, 43(4): 418-420. doi: 10.13926/j.cnki.apps.2013.04.012
    [4] 李社增, 周洪友, 鹿秀云, 等. 中国七省(自治区)马铃薯黄萎病病情及优势病原菌致病力分析[J]. 植物病理学报, 2018, 48(5): 656-665. doi: 10.13926/j.cnki.apps.000226

    LI S Z, ZHOU H Y, LU X Y, et al. Occurrence of potato Verticillium wilt and analysis of pathogenicity differentiation of the major pathogen in seven provinces of China[J]. Acta Phytopathol Sin, 2018, 48(5): 656-665. doi: 10.13926/j.cnki.apps.000226
    [5] 景瑞, 赵方杰, 刘一凡, 等. 宁夏马铃薯黄萎病病原菌分离鉴定及寄主范围测定[J]. 植物病理学报, 2019, 49(1): 11-19. doi: 10.13926/j.cnki.apps.000191

    JING R, ZHAO F J, LIU Y F, et al. Isolation and identification of potato Verticillium wilt pathogen in Ningxia and its host range[J]. Acta Phytopathol Sin, 2019, 49(1): 11-19. doi: 10.13926/j.cnki.apps.000191
    [6] 张成礼. 马铃薯黄萎病的发生与防治[J]. 植物医生, 2004, 17(5): 6. doi: 10.3969/j.issn.1007-1067.2004.05.002

    Zhang C L. Occurrence and control of potato Verticillium wilt[J]. Plant Doc, 2004, 17(5): 6. doi: 10.3969/j.issn.1007-1067.2004.05.002
    [7] HILTUNEN L H, LAAKSO I, CHOBOT V, et al. Influence of thaxtomins in different combinations and concentrations on growth of micropropagated potato shoot cultures[J]. J Agric Food Chem, 2006, 54(9): 3372-3379. doi: 10.1021/jf060270m
    [8] 聂峰杰, 陈虞超, 巩檑, 等. 马铃薯疮痂病致病链霉菌分类及其致病机理研究进展[J]. 分子植物育种, 2018, 16(4): 1313-1319. doi: 10.13271/j.mpb.016.001313

    NIE F J, CHEN Y C, GONG L, et al. Research progress on classification and pathogenesis of Streptomyces spp of potato scab[J]. Mol Plant Breed, 2018, 16(4): 1313-1319. doi: 10.13271/j.mpb.016.001313
    [9] 白晓东, 杜珍, 范向斌, 等. 基质对马铃薯疮痂病抑制效果研究初报[J]. 中国马铃薯, 2002, 16(6): 332-334. doi: 10.3969/j.issn.1672-3635.2002.06.003

    BAI X D, DU Z, FAN X B, et al. A study on control over streptomyces scabies of virus-free microtuber with substratum[J]. Chin Potato, 2002, 16(6): 332-334. doi: 10.3969/j.issn.1672-3635.2002.06.003
    [10] 申建芳, 李子桀, 蒙春燕, 等. 马铃薯黄萎病生防细菌的筛选与鉴定[J]. 北方农业学报, 2018, 46(1): 81-84. doi: 10.3969/j.issn.2096-1197.2018.01.16

    SHEN J F, LI Z J, MENG C Y, et al. Screening and identification of biocontrol bacteria against Verticillium wilt of potato[J]. J North Agric, 2018, 46(1): 81-84. doi: 10.3969/j.issn.2096-1197.2018.01.16
    [11] 赵卫松, 郭庆港, 张晓云, 等. 解淀粉芽胞杆菌PHODG36菌剂的研制及其对马铃薯黄萎病的防病增产效果[J]. 中国生物防治学报, 2020, 36(3): 381-387. doi: 10.16409/j.cnki.2095-039x.2020.03.014

    ZHAO W S, GUO Q G, ZHANG X Y, et al. Development of microbial agent Bacillus amyloliquefaciens PHODG36 and its effect on disease control and yield increase of potato[J]. Chin J Biol Control, 2020, 36(3): 381-387. doi: 10.16409/j.cnki.2095-039x.2020.03.014
    [12] 李玉聪, 李滨影, 油心怡, 等. 马铃薯疮痂病拮抗菌的筛选鉴定及防治效果初探[J]. 生物技术通报, 2018, 34(10): 116-121. doi: 10.13560/j.cnki.biotech.bull.1985.2018-0417

    LI Y C, LI B Y, YOU X Y, et al. Screening, identification and biocontrol effect of antagonistic bacteria on potato common scab[J]. Biotechnol Bull, 2018, 34(10): 116-121. doi: 10.13560/j.cnki.biotech.bull.1985.2018-0417
    [13] 陈志谊, 刘永峰, 刘邮洲, 等. 植物病害生防芽孢杆菌研究进展[J]. 江苏农业学报, 2012, 28(5): 999-1006.

    CHEN Z Y, LIU Y F, LIU Y Z, et al. Research progress in biocontrol of Bacillus spp. against plant diseases[J]. Jiangsu J Agric Sci, 2012, 28(5): 999-1006.
    [14] 刘振华, 罗远婵, 张道敬, 等. 农用微生物杀菌剂剂型研究进展[J]. 农药学学报, 2014, 16(5): 497-507. doi: 10.3969/j.issn.1008-7303.2014.05.02

    LIU Z H, LUO Y C, ZHANG D J, et al. Research progress and prospects of microbial pesticide formulation for plant disease control[J]. Chin J Pestic Sci, 2014, 16(5): 497-507. doi: 10.3969/j.issn.1008-7303.2014.05.02
    [15] 贾丽苑, 贾雨, 黄建新. 拮抗菌应用于微生物杀菌剂的研究现状及展望[J]. 安徽农学通报(下半月刊), 2012, 18(10): 49-52.

    JIA L Y, JIA Y, HUANG J X. The application of antagonistic bacterium to microbial fungicides[J]. Anhui Agric Sci Bull, 2012, 18(10): 49-52.
    [16] 邹丽文, 李婷婷, 付波, 等. 木霉菌·芽孢杆菌混剂对水稻纹枯病的田间防治效果[J]. 上海交通大学学报(农业科学版), 2019, 37(6): 1-5.

    ZOU L W, LI T T, FU B, et al. Control effects of mixed agent of Trichoderma-Bacillus on rice sheath blight[J]. J Shanghai Jiaotong Univ(Agric Sci), 2019, 37(6): 1-5.
    [17] 田永永, 陈立, 谢云, 等. 甜瓜枯萎病拮抗细菌的筛选及大田防效试验[J]. 中国农学通报, 2011, 27(5): 367-371.

    TIAN Y Y, CHEN L, XIE Y, et al. Selection of antagonistic bacteria to melon Fusarium wilt and their biocontrol effect in fields[J]. Chin Agric Sci Bull, 2011, 27(5): 367-371.
    [18] 中华人民共和国农业部. 细菌微生物农药 枯草芽孢杆菌第1部分: 枯草芽孢杆菌母药: NY/T 2293.1—2012[S]. 北京: 中国农业出版社, 2012.

    Ministry of Agriculture of the People's Republic of China. Bacterial pesticides—Bacillus subtilis—Part 1: Bacillus subtilis technical concentrates(TK): NY/T 2293.1—2012[S]. Beijing: China Agriculture Press, 2012.
    [19] 国家技术监督局. 农药pH值的测定方法: GB/T 1601—1993[S]. 北京: 中国标准出版社, 1993.

    The State Bureau of Quality and Technical Supervision. Determination method of pH value for pesticides: GB/T 1601—1993[S]. Beijing: Standards Press of China, 1993.
    [20] 国家技术监督局. 农药粉剂、可湿性粉剂细度测定方法: GB/T 16150—1995[S]. 北京: 中国标准出版社, 1995.

    The State Bureau of Quality and Technical Supervision. Seive test for dustable and wettable powders of pesticides: GB/T 16150—1995[S]. Beijing: Standards Press of China, 1995.
    [21] 中华人民共和国国家质量监督检验检疫总局. 农药可湿性粉剂润湿性测定方法: GB/T 5451—2001[S]. 北京: 中国标准出版社, 2001.

    General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. Testing method for the wettability of dispersible powders of pesticides: GB/T 5451—2001[S]. Beijing: Standards Press of China, 2001.
    [22] 中华人民共和国国家质量监督检验检疫总局. 农药悬浮率测定方法: GB/T14825—2006 [S]. 北京: 中国标准出版社, 2007.

    General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. Determination method of suspensibility for pesticides: GB/T 14825—2006[S]. Beijing: Standards Press of China, 2007.
    [23] 李姝江, 方馨玫, 曾艳玲, 等. 解淀粉芽孢杆菌BA-12可湿性粉剂研制及对核桃根腐病的防治效果[J]. 中国生物防治学报, 2016, 32(5): 619-626. doi: 10.16409/j.cnki.2095-039x.2016.05.011

    LI S J, FANG X M, ZENG Y L, et al. Preparation of wettable powder of Bacillus amyloliquefaciens BA-12 and its control effect on walnut root rot[J]. Chin J Biol Control, 2016, 32(5): 619-626. doi: 10.16409/j.cnki.2095-039x.2016.05.011
    [24] 钱一鑫, 康冀川, 罗乙凯, 等. 猕猴桃灰霉病拮抗解淀粉芽孢杆菌X17可湿性粉剂的研制[J]. 中国生物防治学报, 2016, 32(3): 342-348. doi: 10.16409/j.cnki.2095-039x.2016.03.009

    QIAN Y X, KANG J C, LUO Y K, et al. Development of wettable powder of Bacillus amyloliquefaciens X17 for control of Botrytis cinerea on kiwifruits[J]. Chin J Biol Control, 2016, 32(3): 342-348. doi: 10.16409/j.cnki.2095-039x.2016.03.009
    [25] 中国农药信息网[DB/OL]. http://www.chinapesticide.org.cn/hysj/index.jhtml

    China pesticide information network[DB/OL]. http://www.chinapesticide.org.cn/hysj/index.jhtml
    [26] 贺振宁, 李海燕, 苏媛, 等. 枯草芽孢杆菌B1514可湿性粉剂的研制及其对小麦纹枯病的防效[J]. 河南农业科学, 2015, 44(11): 67-72. doi: 10.15933/j.cnki.1004-3268.2015.11.016

    HE Z N, LI H Y, SU Y, et al. Development of wettable powder of Bacillus subtilis B1514 and its control effect on wheat sheath blight[J]. J Henan Agric Sci, 2015, 44(11): 67-72. doi: 10.15933/j.cnki.1004-3268.2015.11.016
    [27] 王震, 谢晓霞, 施晓艳, 等. 有机番茄生产中“10亿芽孢/克枯草芽孢杆菌可湿性粉剂”的应用效果[J]. 食品安全导刊, 2017(33): 144-145. doi: 10.3969/j.issn.1674-0270.2017.33.104

    WANG Z, XIE X X, SHI X Y, et al. Application effect of “1 billion spores/g Bacillus subtilis wettable powder” in organic tomato production[J]. China Food Saf Mag, 2017(33): 144-145. doi: 10.3969/j.issn.1674-0270.2017.33.104
    [28] 李磊, 许敏, 王美琴. 生防菌解淀粉芽胞杆菌Ht-q6可湿性粉剂的研制及对番茄病害的田间防效[J]. 中国生物防治学报, 2018, 34(5): 738-745. doi: 10.16409/j.cnki.2095-039x.2018.05.0013

    LI L, XU M, WANG M Q. Preparation of biocontrol agent Bacillus amyloliquefaciens Ht-q6 wettable powder and the field control against tomato diseases[J]. Chin J Biol Control, 2018, 34(5): 738-745. doi: 10.16409/j.cnki.2095-039x.2018.05.0013
    [29] 程立君, 陈玉惠, 彭述敏, 等. 1株生防木霉菌可湿性粉剂中助剂的筛选[J]. 西南林学院学报, 2010, 30(5): 48-52.

    CHENG L J, CHEN Y H, PENG S M, et al. Preliminary study on adjuvant selection for a bio-control Trichoderma atroviride power formulation[J]. J Southwest For Univ, 2010, 30(5): 48-52.
    [30] 郭庄园, 杨成德, 金梦军, 等. 枯草芽胞杆菌262XY2´可湿性粉剂的研制[J]. 中国生物防治学报, 2022, 38(2): 414-420. doi: 10.16409/j.cnki.2095-039x.2022.02.011

    GUO Z Y, YANG C D, JIN M J, et al. Development of wettable powder formulation of Bacillus subtilis 262XY2´[J]. Chin J Biol Control, 2022, 38(2): 414-420. doi: 10.16409/j.cnki.2095-039x.2022.02.011
    [31] 李勇, 郭凤柳, 赵伟全, 等. 马铃薯疮痂病菌拮抗菌ZWQ-1的鉴定及防效测定[J]. 河南农业科学, 2012, 41(10): 94-99. doi: 10.3969/j.issn.1004-3268.2012.10.022

    LI Y, GUO F L, ZHAO W Q, et al. Identification and control efficiency of antagonistic strain ZWQ-1 against potato scab pathogens[J]. J Henan Agric Sci, 2012, 41(10): 94-99. doi: 10.3969/j.issn.1004-3268.2012.10.022
  • 加载中
计量
  • 文章访问数:  40
  • HTML全文浏览量:  8
  • 被引次数: 0
出版历程
  • 收稿日期:  2022-06-17
  • 录用日期:  2022-08-15
  • 网络出版日期:  2022-09-20

目录

    /

    返回文章
    返回