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波尔多液对烟草叶际微生物群落结构与代谢功能的影响

刘亭亭 汪汉成 孙美丽 尹国英 张盼 蔡刘体 向立刚 孟建玉 张长青

刘亭亭, 汪汉成, 孙美丽, 尹国英, 张盼, 蔡刘体, 向立刚, 孟建玉, 张长青. 波尔多液对烟草叶际微生物群落结构与代谢功能的影响[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0087
引用本文: 刘亭亭, 汪汉成, 孙美丽, 尹国英, 张盼, 蔡刘体, 向立刚, 孟建玉, 张长青. 波尔多液对烟草叶际微生物群落结构与代谢功能的影响[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0087
LIU Tingting, WANG Hancheng, SUN Meili, YIN Guoying, ZHANG Pan, CAI Liuti, XIANG Ligang, MENG Jianyu, ZHANG Changqing. Effects of Bordeaux mixture on the community structure and metabolic function of tobacco phyllosphere microorganisms[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0087
Citation: LIU Tingting, WANG Hancheng, SUN Meili, YIN Guoying, ZHANG Pan, CAI Liuti, XIANG Ligang, MENG Jianyu, ZHANG Changqing. Effects of Bordeaux mixture on the community structure and metabolic function of tobacco phyllosphere microorganisms[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0087

波尔多液对烟草叶际微生物群落结构与代谢功能的影响

doi: 10.16801/j.issn.1008-7303.2022.0087
基金项目: 国家自然科学基金 (31960550,32160522);贵州省科技基金项目 (黔科合基础-ZK[2021]重点036);中国烟草总公司科技项目 (110202001035 (LS-04),110202101048 (LS-08) );贵州省“百层次”创新型人才 (黔科合平台人才-GCC[2022]028-1)。
详细信息
    作者简介:

    刘亭亭,3026049684@qq.com

    通讯作者:

    汪汉成,xiaobaiyang126@hotmail.com

  • 中图分类号: S482.2;X592

Effects of Bordeaux mixture on the community structure and metabolic function of tobacco phyllosphere microorganisms

Funds: National Natural Science Foundation of China (31960550, 32160522); Guizhou Science and Technology Fund Project (The basis of Guizhou Science and Technology Cooperation-ZK [2021] Key 036); Technology Project of China National Tobacco Corporation (110202001035 (LS-04), 110202101048 (LS-08)); Guizhou Province "100 level" innovative talents (Qian Science Cooperation Platform talents-GCC [2022] 028-1).
  • 摘要: 测定了波尔多液对烟草赤星病菌的毒力,并采用高通量测序与Biolog代谢表型技术分别测定了其对烟叶健康与感病组织叶际微生物群落结构和代谢功能的影响。结果表明:波尔多液对烟草赤星病菌的抑制活性较弱,其抑制菌丝生长和孢子萌发的EC50值分别为450.19和757.17 mg/L。健康与感病烟叶组织叶际细菌均分布于变形菌门 (6.93%和39.07%) 和厚壁菌门 (16.45%和0.65%),优势细菌均有Kosakonia (3.46%和22.38%) 和假单胞菌属 (0.22%和5.95%);真菌均分布于子囊菌门 (63.82%和93.74%) 和担子菌门 (6.82%和2.53%),优势真菌有链格孢属 (36.48%和84.52%) 、Symmetrospora (5.56%和2.27%) 和枝孢霉属 (14.87%和6.66%)。波尔多液1 500 g/hm2处理对健康和感病烟叶叶际细菌和真菌群落结构与代谢功能均有影响,处理5 d时降低了叶际Kosakonia、鞘脂单胞菌属和乳杆菌属的相对丰度,增加了假单胞菌属、劳尔氏菌属等6种细菌菌属的相对丰度;降低了链格孢属、Symmetrospora等6种真菌属的相对丰度,增加了亚隔孢壳属、绿僵菌属等10种真菌属的相对丰度。处理10和15 d时对叶际真菌、细菌的影响逐渐降低。健康与感病烟叶叶际微生物均可高效代谢糖类、氨基酸类、羧酸类、双亲化合物、聚合物和胺/氨基化合物等29种碳源,但对α-丁酮酸的代谢较弱。波尔多液处理对烟叶叶际微生物的代谢抑制活性随时间延长逐渐减弱。研究结果揭示了波尔多液施用不同时期后对烟叶叶际微生物的影响规律,为了解药剂持效期的生态效益提供了参考依据。
  • 图  1  波尔多液对烟叶叶际细菌门 (A) 和属(B)水平上的群落组成的影响

    Figure  1.  The effects of Bordeaux mixture on the community composition of bacteria in the phyllosphere of tobacco at phyla(A) and genera(B) levels

    图  2  波尔多液处理对烟叶叶际真菌门 (A) 和属(B)水平上的群落组成的影响

    Figure  2.  The effects of Bordeaux mixture on the community composition of fungi in the phyllosphere of tobacco at phyla(A) and genera(B) levels

    图  3  波尔多液处理烟叶叶际细菌 (A) 和真菌(B)群落OTU分布花瓣图

    Figure  3.  Flower map of OTU distribution of bacterial(A) and fungal(B) communities in phyllosphere of tobacco treated with Bordeaux mixture

    图  4  波尔多液处理烟叶叶际微生物代谢功能的聚类热图

    注:颜色值分别代表微生物代谢碳源的程度,0~100为代谢程度低,100~200为代谢程度一般,200以上为代谢程度高。

    Figure  4.  Cluster heat map of the effect of Bordeaux mixture on the metabolism of tobacco phyllosphere microorganism

    Note: The color values represent the degree of the microbes metabolizing carbon sources. The value 0-100, 100-200, and >200 represent the leaf microbes metabolized in the Biolog ECO microplate poorly, moderately, and effectively, respectively.

    表  1  样品采集信息

    Table  1.   Samples information

    取样部位
    Sampling location
    施药前0 d
    0 day before application
    施药后5 d
    5 days after application
    施药后10 d
    10 days after application
    施药后15 d
    15 days after application
    健康组织
    Healthy tissue
    样品编号
    Sample No.
    BEJ01 BEJ11 BEJ21 BEJ31
    BEJ02 BEJ12 BEJ22 BEJ32
    BEJ03 BEJ13 BEJ23 BEJ33
    分组编号
    Group No.
    BEJ0 BEJ1 BEJ2 BEJ3
    感病组织
    Diseased tissue
    样品编号
    Sample No.
    BEB01 BEB11 BEB21 BEB31
    BEB02 BEB12 BEB22 BEB32
    BEB03 BEB13 BEB23 BEB33
    分组编号
    Group No.
    BEB0 BEB1 BEB2 BEB3
    下载: 导出CSV

    表  2  波尔多液对烟草赤星病菌的毒力

    Table  2.   Toxicity test of Bordeaux mixture to Alternaria alternata

    质量浓度
    Mass concentration/(mg/L)
    抑制率
    Inhibition ratio/%
    EC50/(mg/L) EC95/(mg/L) 回归方程
    Regression equation
    相关系数
    r
    菌丝生长
    Mycelial growth
    00450.194987.45y=4.26 + 1.61x0.992
    10015.99
    20025.17
    40049.37
    80066.34
    160079.56
    孢子萌发
    Conidia germination
    00757.173108.01y=7.67 + 2.67x0.997
    2006.74
    40022.80
    80049.97
    160082.41
    下载: 导出CSV

    表  3  波尔多液对烟叶叶际微生物多样性的影响 (OTU水平)

    Table  3.   Effect of Bordeaux mixture on the diversity of tobacco phyllosphere microorganism (OTU level)

    分组
    Group
    香农指数
    Shannon index
    Chao1 指数
    Chao1 index
    ACE 指数
    ACE index
    覆盖度
    Goods coverage
    细菌 BacteriaBEB01.24±0.54 bc20.00±6.81 b29.88±10.97 b0.98±0.01 a
    BEJ01.26±0.08 bc46.44±25.14 ab41.69±12.65 b0.97±0.01 a
    BEB12.35±0.09 ab46.33±13.86 ab48.09±14.73 b0.96±0.01 a
    BEJ10.73±0.19 c71.75±39.56 ab74.87±39.93 b0.95±0.02 ab
    BEB22.78±1.11 a97.54±29.63 ab120.30±31.45 ab0.90±0.03 b
    BEJ21.53±0.19 abc130.98±40.17 a212.81±45.16 a0.90±0.01 b
    BEB31.57±0.14 abc36.67±13.32 b48.14±21.83 b0.96±0.02 a
    BEJ31.63±0.50 abc70.34±43.99 ab90.39±55.60 b0.95±0.02 ab
    真菌 FungiBEB00.99±0.42 c64.89±6.18 e70.36±5.40 e0.999±0.000 a
    BEJ03.17±0.16 b193.26±58.62 de208.14±66.06 de0.998±0.001 ab
    BEB11.37±0.22 c218.21±42.63 d253.44±63.40 cd0.997±0.001 bc
    BEJ14.22±0.69 ab384.51±46.54 c375.92±57.74 c0.997±0.000 bc
    BEB23.48±0.42 ab572.71±19.17 b668.60±24.86 a0.992±0.001 d
    BEJ24.59±0.30 a633.01±33.59 ab644.18±56.44 ab0.992±0.001 d
    BEB34.37±0.77 ab728.86±55.29 a724.05±42.94 a0.992±0.001 d
    BEJ34.69±0.26 a521.60±52.80 b523.98±32.61 b0.995±0.001 c
    注:表中同列数据后相同字母表示采用DPS中LSD法检验差异不显著 (P>0.05)。Note: Date followed by the same letter in the same column are not significantly different by the least significant difference test (LSD) in DPS (P>0.05).
    下载: 导出CSV
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  • 收稿日期:  2022-05-05
  • 录用日期:  2022-08-22
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