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啶酰菌胺等8种杀菌剂对烟草叶斑病菌的生物活性

汪汉成 黄宇 杨金初 李治模 蔡刘体 韦克苏 孟建玉 李忠

汪汉成, 黄宇, 杨金初, 李治模, 蔡刘体, 韦克苏, 孟建玉, 李忠. 啶酰菌胺等8种杀菌剂对烟草叶斑病菌的生物活性[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0092
引用本文: 汪汉成, 黄宇, 杨金初, 李治模, 蔡刘体, 韦克苏, 孟建玉, 李忠. 啶酰菌胺等8种杀菌剂对烟草叶斑病菌的生物活性[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0092
WANG Hancheng, HUANG Yu, YANG Jinchu, LI Zhimo, CAI Liuti, WEI Kesu, MENG Jianyu, LI Zhong. Bioactivities of boscalid, etc. eight fungicides against Didymella segeticola in tobacco[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0092
Citation: WANG Hancheng, HUANG Yu, YANG Jinchu, LI Zhimo, CAI Liuti, WEI Kesu, MENG Jianyu, LI Zhong. Bioactivities of boscalid, etc. eight fungicides against Didymella segeticola in tobacco[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0092

啶酰菌胺等8种杀菌剂对烟草叶斑病菌的生物活性

doi: 10.16801/j.issn.1008-7303.2022.0092
基金项目: 中国烟草总公司科技项目[110202101048 (LS-08),110202001035 (LS-04) ];贵州省“百层次”创新型人才 (GCC[2022]028-1);国家自然科学基金[32160522];贵州省科技计划项目 (黔科合基础-ZK[2021]重点036);中国烟草总公司贵州省公司科技项目 (2020XM022).
详细信息
    通讯作者:

    汪汉成,xiaobaiyang126@hotmail.com

    蔡刘体,cailiuti01@163.com

  • 中图分类号: S482.2;S481.4;TQ450.21

Bioactivities of boscalid, etc. eight fungicides against Didymella segeticola in tobacco

Funds: China National Tobacco Corporation (110202101048(LS-08), 110202001035(LS-04), 110202101048(LS-08)), the “Hundred” Level Innovative Talent Foundation of Guizhou Province (GCC[2022]028-1), National Natural Science Foundation of China (32160522), Guizhou Science Technology Foundation (ZK[2021]Key036), and Guizhou Tobacco Company (2020XM22).
  • 摘要: 采用生物测定方法分析了烟草叶斑病菌Didymella segeticola在菌丝生长阶段对8种杀菌剂 (啶酰菌胺、苯醚甲环唑、丙环唑、氟硅唑、多菌灵、咪鲜胺、菌核净和代森锰锌) 的敏感性,同时通过离体叶片法测定了8种杀菌剂对烟草叶斑病的保护和治疗作用。结果表明:供试8种杀菌剂对D. segeticola菌丝生长表现出不同的抑菌活性,同时对其引起的病害具有一定的保护和治疗作用。抑菌活性最强的是啶酰菌胺,其平均EC50值为 (0.0470 ± 0.0120) mg/L;其次依次为苯醚甲环唑[ (0.0790 ± 0.0050) mg/L]、咪鲜胺[ (0.29 ± 0.08) mg/L]、丙环唑[ (0.69 ± 0.12) mg/L]、菌核净[ (1.08 ± 0.33) mg/L]、多菌灵[ (1.22 ± 0.29) mg/L]、氟硅唑[ (1.38 ± 0.07) mg/L];代森锰锌的抑菌活性最弱[ (22.80 ± 10.51) mg/L]。进一步研究表明,氟硅唑、苯醚甲环唑、丙环唑、啶酰菌胺、菌核净和多菌灵对烟草叶斑病保护作用较强,25 mg/L药剂质量浓度处理下防效均 >82%;100 mg/L代森锰锌处理下防效为70.51%。啶酰菌胺和氟硅唑对叶斑病具有较好的治疗活性,25 mg/L下防效均 >80%;其次为咪鲜胺、苯醚甲环唑、多菌灵和菌核净;代森锰锌的治疗作用较差,100 mg/L下的防效仅为63.31%。研究结果可为烟草叶斑病防治药剂筛选提供参考和依据。
  • 表  1  8种杀菌剂对Didymella segeticola菌丝生长的抑制作用

    Table  1.   Inhibitory effects of eight fungicides on the mycelial growth of Didymella segeticola

    杀菌剂
    Fungicide
    菌株
    Isolate
    毒力回归方程
    Toxicity regression equation
    相关系数
    r
    EC50/
    (mg/L)
    EC50平均值
    Average value of EC50/(mg/L)
    啶酰菌胺 boscalid YBZ 121 y = 1.80x + 2.29 0.97 0.0550 0.0470±0.0120 a
    YBZ 221 y = 2.16x + 2.76 0.96 0.0530
    YBZ 111 y = 2.92x + 4.28 0.96 0.0330
    苯醚甲环唑 difenoconazole YBZ 121 y = 1.13x + 1.17 0.97 0.0850 0.0790±0.0050 a
    YBZ 221 y = 1.32x + 1.47 0.98 0.0780
    YBZ 111 y = 1.39x + 1.59 0.98 0.0750
    丙环唑 propiconazole
    YBZ 121 y = 0.76x + 0.10 0.99 0.73 0.69±0.12 b
    YBZ 221 y = 0.87x + 0.09 0.99 0.79
    YBZ 111 y = 0.98x + 0.25 0.99 0.56
    氟硅唑 flusilazole YBZ 121 y = 1.08x − 0.16 0.99 1.41 1.38±0.07 c
    YBZ 221 y = 0.84x − 0.13 0.98 1.42
    YBZ 111 y = 1.16x − 0.13 0.99 1.30
    多菌灵 carbendazim YBZ 121 y = 3.88x − 0.37 0.99 1.23 1.22±0.29 c
    YBZ 221 y = 5.55x − 0.96 0.99 1.51
    YBZ 111 y = 5.05x + 0.19 0.99 0.93
    咪鲜胺 prochloraz YBZ 121 y = 0.96x + 0.64 0.99 0.22 0.29±0.08 b
    YBZ 221 y = 1.04x + 0.44 0.98 0.38
    YBZ 111 y = 1.60x + 0.94 0.97 0.26
    菌核净 dimethachlon YBZ 121 y = 2.26x + 0.10 0.97 0.94 1.08±0.33 c
    YBZ 221 y = 0.68x + 0.11 0.99 1.45
    YBZ 111 y = 2.07x + 0.21 0.97 0.84
    代森锰锌 mancozeb YBZ 121 y = 1.30x − 1.44 0.99 12.88 22.80±10.51 d
    YBZ 221 y = 0.53x − 0.71 0.99 21.71
    YBZ 111 y = 1.12x − 1.71 0.97 33.82
    注:表中不同小写字母表示处理间存在显著性差异 (P<0.05)。Note: Data with different lowercase letters presented there was significant difference between treatments (P<0.05).
    下载: 导出CSV

    表  2  8种杀剂对烟草Didymella segeticola叶斑病的保护和治疗作用

    Table  2.   Protective and curative effects of eight fungicides against Didymella segeticola leaf spot

    杀菌剂
    Fungicide
    质量浓度
    Mass conc./
    (mg/L)
    保护作用防效
    Protective
    efficacy/
    %
    治疗作用防效
    Curative
    efficacy/
    %
    啶酰菌胺 boscalid 100 93.50 ab 87.40 ab
    25 87.84 bc 82.86 ab
    苯醚甲环唑 difenoconazole 100 94.35 ab 89.20 a
    25 89.63 bc 76.00 b
    丙环唑 propiconazole 100 94.12 ab 91.82 a
    25 85.83 bc 63.23 c
    氟硅唑 flusilazole 100 95.81 ab 84.60 ab
    25 90.90 bc 80.41 ab
    多菌灵 carbendazim 100 95.52 ab 82.43 ab
    25 82.74 c 77.52 b
    咪鲜胺 prochloraz 200 79.90 c 87.62 ab
    50 73.12 d 85.24 ab
    菌核净 dimethachlon 100 99.42 a 78.93 ab
    25 88.60 bc 54.22 c
    代森锰锌 mancozeb 400 88.00 bc 79.50 ab
    100 70.51 d 63.31 c
    注:表中不同小写字母表示处理间存在显著性差异 (P<0.05)。Note: Data with different lowercase letters presented there was significant difference between treatments (P<0.05)
    下载: 导出CSV
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  • 收稿日期:  2021-09-05
  • 录用日期:  2022-05-05
  • 网络出版日期:  2022-08-29

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