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甘薯长喙壳菌对咯菌腈的敏感基线及咯菌腈对甘薯黑斑病的防治效果

张德胜 乔奇 白瑞英 田雨婷 王爽 王永江 赵付枚 张振臣

张德胜, 乔奇, 白瑞英, 田雨婷, 王爽, 王永江, 赵付枚, 张振臣. 甘薯长喙壳菌对咯菌腈的敏感基线及咯菌腈对甘薯黑斑病的防治效果[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0094
引用本文: 张德胜, 乔奇, 白瑞英, 田雨婷, 王爽, 王永江, 赵付枚, 张振臣. 甘薯长喙壳菌对咯菌腈的敏感基线及咯菌腈对甘薯黑斑病的防治效果[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0094
ZHANG Desheng, QIAO Qi, BAI Ruiying, TIAN Yuting, WANG Shuang, WANG Yongjiang, ZHAO Fumei, ZHANG Zhenchen. Sensitivity baseline of Ceratocystis fimbriata to fludioxonil and control efficacy of fludioxonil against black rot of sweet potato[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0094
Citation: ZHANG Desheng, QIAO Qi, BAI Ruiying, TIAN Yuting, WANG Shuang, WANG Yongjiang, ZHAO Fumei, ZHANG Zhenchen. Sensitivity baseline of Ceratocystis fimbriata to fludioxonil and control efficacy of fludioxonil against black rot of sweet potato[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0094

甘薯长喙壳菌对咯菌腈的敏感基线及咯菌腈对甘薯黑斑病的防治效果

doi: 10.16801/j.issn.1008-7303.2022.0094
基金项目: 国家现代农业产业技术体系资助 (CARS-10-B13);河南省科技攻关 (212102110458);河南省农业科学院自主创新基金 (2021ZC38).
详细信息
    作者简介:

    张德胜,zhangdesheng404@163.com

    通讯作者:

    张振臣,zhangzhenchen@126.com

  • 中图分类号: S482.2;S481.4

Sensitivity baseline of Ceratocystis fimbriata to fludioxonil and control efficacy of fludioxonil against black rot of sweet potato

Funds: Supported by China Agriculture Research System of MOF and MARA(CARS-10-B13); Science and Technology Projects of henan province(212102110458); Independent Innovation Foundation of Henan Academy of Agricultural Sciences(2021ZC38)
  • 摘要: 为探索甘薯长喙壳菌Ceratocystis fimbriata 对咯菌腈的敏感性,明确咯菌腈在苗期和储藏期对甘薯黑斑病的防治效果,分别从中国河南、四川、河北、山东采集并分离得到64株甘薯长喙壳菌。采用凹玻片法观察了咯菌腈对病原菌分生孢子萌发和芽管伸长的影响,采用孢子萌发法和菌丝生长速率法测定了64个菌株对咯菌腈的敏感性,并对50% 咯菌腈可湿性粉剂 (WP) 防治苗期和储藏期甘薯黑斑病的效果进行了评价。结果表明:随咯菌腈处理浓度增加,甘薯长喙壳菌分生孢子的萌发率逐渐下降,芽管扭曲程度逐渐加大。咯菌腈还可造成芽管过早的出现分支。咯菌腈对供试菌株分生孢子萌发的EC50值在0.20~0.99 μg/mL,平均值为0.52 μg/mL;对菌丝生长的EC50值在0.17~0.31 μg/mL,平均值为0.24 μg/mL,不同敏感性菌株所占频率呈正态分布,可作为甘薯长喙壳菌对咯菌腈的敏感基线。50% 咯菌腈WP 在有效成分500 mg/L 下浸苗处理,对薯苗黑斑病的防治效果可达90.24%,显著高于500 g/L甲基硫菌灵悬浮剂 (SC) 同浓度下的处理。采用浸薯块法防治储藏期黑斑病,50% 咯菌腈WP 500 mg/L连续两年的防治效果分别达到84.41%和82.30%,也显著高于同浓度的甲基硫菌灵。药剂处理后60和90 d,甘薯薯块中咯菌腈的残留量低于其最大残留限量 (MRL) 标准。本研究表明,咯菌腈具有防治甘薯生长期和储藏期黑斑病的潜力。
  • 图  1  咯菌腈对甘薯长喙壳菌分生孢子萌发的影响

    A:清水对照 (9 h);B:咯菌腈0.5 μg/mL (9 h);C:咯菌腈1 μg/mL (9 h);D:咯菌腈2 μg/mL (20 h)

    Figure  1.  Effects of fludioxonil on conidial germination of C. fimbriata

    A: CK (9 h);B: fludioxonil 0.5 μg/mL (9 h);C: fludioxonil 1 μg/mL (9 h);D: fludioxonil 2 μg/mL (20 h)

    图  2  咯菌腈对甘薯长喙壳菌菌丝生长的影响

    A:清水对照 (9 h);B:咯菌腈0.3 μg/mL (9h);C:咯菌腈0.5 μg/mL (9 h);D:咯菌腈0.5 μg/mL (9 h)

    Figure  2.  Effects of fludioxonil on mycelium growth of C. fimbriata

    A: CK (9 h);B: fludioxonil 0.3 μg/mL (9 h);C: fludioxonil 0.5 μg/mL (9 h);D: fludioxonil 0.5 μg/mL (9 h)

    图  3  甘薯长喙壳菌孢子萌发 (A) 和菌丝生长 (B) 对咯菌腈敏感性频率分布

    Figure  3.  Frequency distribution of sensitivity on conidial germination(A) and mycelium growth(B) of C. fimbriata to fludioxonil

    表  1  咯菌腈对苗期黑斑病的防治效果

    Table  1.   The control efficacy of fludioxonil to black rot of sweet potato seedlings

    药剂
    Fungicide
    有效成分剂量
    Dosage, a.i./(mg/L)
    病株率
    Disease incidence/%
    防治效果
    Control efficacy/%
    50株薯苗总鲜重
    Total fresh weight of 50 seedlings/kg
    50% 咯菌腈WP
    fludioxonil 50% WP
    2504.6782.93±5.19ab2.05±0.07b
    5002.6790.24±2.76a2.08±0.07b
    500 g/L 甲基硫菌灵
    SC thiophanate-methyl 500 g/L SC
    5006.0078.05±3.94b2.27±0.09a
    CK27.331.96±0.05b
    注:同列数据后不同小写字母分别表示经新复极差法比较在0.05水平差异显著。Note: Different lowercase letters in the same column indicate significant difference at 0.05 level by the new multiple range test.
    下载: 导出CSV

    表  2  咯菌腈对储藏期黑斑病的防治效果

    Table  2.   The control efficacy of fludioxonil to black rot of sweet potato in storage

    药剂
    Fungicide
    有效成分剂量
    Dosage, a.i./(mg/L)
    防治效果
    Control efficacy /%
    20192020
    50% 咯菌腈WP
    fludioxonil 50% WP
    25063.43±5.95b68.03±2.83b
    50084.41±7.25a82.30±1.35a
    500 g/L 甲基硫菌灵SC
    thiophanate-methyl 500g/L SC
    50054.71±9.71b56.31±3.76c
    注:同列数据后不同小写字母分别表示经新复极差法比较在0.05水平差异显著。Note: Different lowercase letters in the same column indicate significant difference at 0.05 level by the new multiple range test.
    下载: 导出CSV

    表  3  施用50% 咯菌腈WP后甘薯块中的残留量及相关标准

    Table  3.   Residual quantity of fludioxonil 50% WP in root tuber of sweet potato and standard of MRL and ADI

    有效成分剂量
    Dosage,a.i. / (mg/L)
    60 d残留
    Residue at 60 d in storage/(mg/kg)
    90 d残留
    Residue at 90 d in storage/(mg/kg)
    最大残留限量[22]
    MRL/(mg/kg)
    每日允许摄入量[22]
    ADI/( mg/kg)
    2501.110.54100.4
    5002.021.37
    7502.391.68
    下载: 导出CSV
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  • 收稿日期:  2021-10-24
  • 录用日期:  2022-04-13
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