灭菌唑与氰烯菌酯复配对水稻恶苗病菌的抑制活性

李美霞; 陈香华; 周长勇; 陈亚丽; 朱春华; 曹凯歌; 王建新; 周明国; 段亚冰

doi:10.16801/j.issn.1008-7303.2022.0134

灭菌唑与氰烯菌酯复配对水稻恶苗病菌的抑制活性

doi: 10.16801/j.issn.1008-7303.2022.0134

1.
江苏徐淮地区淮阴农业科学研究所，江苏淮安 223001
2.
南京农业大学植物保护学院农药系，南京 210095
3.
江苏省南京市六合区春华家庭农场，南京 211500

基金项目: 国家重点研发计划 (2016YFD0200503-04)；南京六合区2021年基层农技推广体系改革与建设补助项目.

详细信息

作者简介:
李美霞，meixia729814@163.com

通讯作者:
段亚冰，dyb@njau.edu.cn

中图分类号: S482.2；TQ450.15；S435.1
计量
- 文章访问数: 242
- HTML全文浏览量: 43
- PDF下载量: 51
- 被引次数: 0
出版历程
- 收稿日期: 2021-11-08
- 录用日期: 2022-05-08
- 网络出版日期: 2022-11-15
- 刊出日期: 2022-12-02

Antifungal activity of the compound of triticonazole and phenamacril against Fusarium fujikuroi

1.
Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Huai'an 223001, Jiangsu Province, China
2.
Department of Pesticide, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
3.
Chunhua Family Farm, Liuhe District, Nanjing City Jiangsu Province, Nanjing 211500, China

Funds: National Key R&D Program of China (2016YFD0200503-04); 2021 Grassroots Agricultural Technology Promotion System Reform and Construction Subsidy Project in Nanjing Liuhe District.

摘要

摘要: 采用菌丝生长速率法，测定了灭菌唑对水稻恶苗病菌的毒力，测得灭菌唑对10株水稻恶苗病菌的EC₅₀值范围为0.0664~0.7661 μg/mL。采用有效成分质量浓度分别为60、120和240 μg/mL的25 g/L灭菌唑种子处理悬浮剂处理水稻种子后，对水稻发芽率、株高、鲜重均无影响。表明灭菌唑对水稻恶苗病菌具有较好的抑制活性，且对水稻安全，可用于水稻恶苗病的化学防控。氰烯菌酯对10株水稻恶苗病菌的EC₅₀值范围为0.0076~0.2629 μg/mL。将氰烯菌酯和灭菌唑分别按质量比4 : 1、3 : 1、2 : 1、1 : 1、1 : 2、1 : 3和1 : 4复配，测定了复配药剂对水稻恶苗病菌的联合毒力，所得增效系数在0.5~1.5之间，均为相加作用。研究表明，将氰烯菌酯与灭菌唑复配后施用，不仅能显著降低单剂的使用剂量，且能降低氰烯菌酯对病原菌群体的选择压力，延缓抗药性的发生和发展速度，保障病害防效和水稻生产安全。
- 水稻恶苗病菌 /
- 灭菌唑 /
- 氰烯菌酯 /
- 联合毒力 /
- 安全 /
- 增效系数
Abstract: In this study, the mycelial growth rate method was used to determine the antifungal activity of triticonazole against Fusarium fujikuroi, and the EC₅₀ values of triticonazole against 10 strains of F. fujikuroi ranged from 0.0664 μg/mL to 0.7661 μg/mL. After seed treatment with 60, 120, and 240 μg/mL active concentrations of 25 g/L triticonazole suspension concentrate, the germination rate, plant height, and fresh weight of rice were not affected. The results indicated that triticonazole not only has good antifungal activity against F. fujikuroi, but also has good security for rice, and can be used to control rice bakanae disease. The EC₅₀ values of phenamacril against 10 strains of F. fujikuroi ranged from 0.0076 to 0.2629 μg/mL. The joint toxicities of phenamacril and triticonazole at the ratios of 4 : 1, 3 : 1, 2 : 1, 1 : 1, 1 : 2, 1 : 3, and 1 : 4 against F. fujikuroi were determined. The result showed the synergistic ratio ranged from 0.5 to 1.5, indicating that the combinations of phenamacril and triticonazole exhibited an additive effect on the inhibition of F. fujikuroi mycelial growth. This study indicated the combinations of phenamacril and triticonazole not only decrease the use dosage of a single component significantly but also reduce the selective pressure of F. fujikuroi populations to phenamacril and delay the resistance development, ensuring the control efficacy of rice bakanae disease and safe production of rice.
- Fusarium fujikuroi /
- triticonazole /
- phenamacril /
- joint toxicity /
- safety /
- synergistic ratio

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表 1 水稻恶苗病菌敏感性测定试验中氰烯菌酯和灭菌唑复配药剂的浓度梯度

Table 1. Concent ration gradients of the combinations of phenamacril and triticonazole for the sensitivity tests of F. fujikuroi

药剂处理 Fungicide treatment	浓度梯度 Concentration gradient/(μg/mL)
氰烯菌酯 phenamacril (PHE)	0，0.0625，0.125，0.25，0.5，1
灭菌唑 triticonazole (TRI)	0，0.039，0.078，0.156，0.3125，0.625，1.25
PHE : TRI (4 : 1)	0, 0.078，0.1556，0.3125，0.625，1.25
PHE : TRI (3 : 1)	0, 0.0125，0.025，0.05，0.1，0.2，0.4
PHE : TRI (2 : 1)	0, 0.05，0.1，0.2，0.4，0.8
PHE : TRI (1 : 1)	0, 0.05，0.1，0.2，0.4，0.8
PHE : TRI (1 : 2)	0, 0.075，0.15，0.3，0.6
PHE : TRI (1 : 3)	0, 0.039，0.078，0.156，0.3125，0.625
PHE : TRI (1 : 4)	0, 0.0125，0.025，0.05，0.1，0.2，0.4

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表 2 水稻恶苗病菌对灭菌唑的敏感性

Table 2. Sensitivity of F. fujikuroi to triticonazole

菌株 Strains	回归方程 Regression equation	相关系数 r	有效抑制中浓度 EC₅₀/(μg/mL)
ZJSX-1	y = 5.6921 + 1.2144x	0.9973	0.2692
ZJSX-4	y = 5.9860 + 1.2590x	0.9982	0.1648
JSHA-6	y = 5.7927 + 0.9376x	0.9823	0.1427
JSHA-8	y = 5.3572 + 1.0823x	0.9949	0.4677
JSHA-11	y = 5.3647 + 1.0261x	0.9979	0.4411
JSHA-13	y = 5.5402 + 1.4438x	0.9934	0.4225
JSTZ-14	y = 5.7115 + 0.7734x	0.9927	0.1181
JSTZ-16	y = 5.8591 + 1.0077x	0.9957	0.1404
JSTZ-17	y = 5.1417 + 1.2248x	0.9939	0.7661
JSTZ-20	y = 6.4429 + 1.2250x	0.9934	0.0664
注：ZJSX-，菌株采自浙江省绍兴地区；JSHA-，菌株采自江苏省淮安地区；JSTZ-，菌株采自江苏省泰州地区。Note: ZJSX-, strains were collected from Shaoxing area in Zhejiang Province. JSHA-, strains were collected from Huai'an area in Jiangsu Province. JSTZ-, strains were collected from Taizhou area in Jiangsu Province.

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表 3 25 g/L灭菌唑种子处理悬浮剂对水稻的安全性

Table 3. Safety of 25 g/L triticonazole FS on rice

药剂有效成分质量浓度 Mass conc. of fungicide, a.i./ (μg/mL)	剂量 Dosage, a.i./ (g/100 kg (seed))	发芽率 Germination rate/%		株高 Height/cm		鲜重 Weight/g
药剂有效成分质量浓度 Mass conc. of fungicide, a.i./ (μg/mL)	剂量 Dosage, a.i./ (g/100 kg (seed))	南粳 5055 Nanjing 5055	苏御糯 Suyunuo	南粳 5055 Nanjing 5055	苏御糯 Suyunuo	南粳 5055 Nanjing 5055	苏御糯 Suyunuo
0	0	87.33 a	85.47 a	38.79 a	58.15 a	0.6233 a	1.0600 a
60	6	88.89 a	87.18 a	38.64 a	58.06 a	0.6067 a	1.0433 a
120	12	84.13 a	88.03 a	38.05 a	57.37 a	0.5000 a	1.0233 a
240	24	85.95 a	85.47 a	37.52 a	57.12 a	0.4833 a	1.0133 a
注：同列数据后相同小写字母表示差异不显著(P>0.05)。Note: Same lowercase indicate no significant difference among the same column data (P>0.05).

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表 4 水稻恶苗病菌对氰烯菌酯的敏感性

Table 4. Sensitivity of F. fujikuroi to phenamacril

菌株 Strains	回归方程 Regression equation	相关系数 r	有效抑制中浓度 EC₅₀/(μg/mL)
ZJSX-5	y = 6.3319 + 0.9324x	0.9997	0.0373
ZJSX-6	y = 5.9331 + 1.0859x	0.9946	0.1383
ZJSX-9	y = 6.0792 + 0.8955x	0.9932	0.0623
ZJSX-11	y = 6.1082 + 1.3201x	0.9932	0.1447
JSHA-13	y = 6.3157 + 1.2705x	0.9984	0.0921
JSHA-16	y = 6.4327 + 1.2801x	0.9991	0.0076
JSTZ-17	y = 5.9844 + 1.0193x	0.9943	0.1082
JSTZ-18	y = 5.6939 + 1.1958x	0.9932	0.2629
JSTZ-21	y = 6.3412 + 1.2273x	0.9966	0.0807
JSTZ-22	y = 6.3151 + 0.8375x	0.9946	0.0269
JSTZ-28	y = 6.3159 + 1.4315x	0.9903	0.1204
注：ZJSX-，菌株采自浙江省绍兴地区；JSHA-，菌株采自江苏省淮安地区；JSTZ-，菌株采自江苏省泰州地区。Note: ZJSX-, strains were collected from Shaoxing area in Zhejiang Province. JSHA-, strains were collected from Huai'an area in Jiangsu Province. JSTZ-, strains were collected from Taizhou area in Jiangsu Province.

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表 5 水稻恶苗病菌对氰烯菌酯和灭菌唑各复配药剂的敏感性

Table 5. Sensitivity of F. fujikuroi to the combinations of phenamacril and triticonazole

药剂处理 Fungicide treatment	回归方程 Regression equation	相关系数 r	有效抑制中浓度 EC₅₀/ (μg/mL)	增效比 SR
氰烯菌酯 phenamacril (PHE)	y = 7.8179 + 3.6309x	0.9914	0.1675	—
灭菌唑 triticonazole (TRI)	y = 5.8103 + 1.1127x	0.9995	0.1870	—
PHE: TRI (1 : 1)	y = 6.3191 + 1.9450x	0.9991	0.2098	0.8423
PHE: TRI (2 : 1)	y = 6.3484 + 1.7800x	0.9922	0.1748	0.9927
PHE: TRI (3 : 1)	y = 6.6721 + 2.0427x	0.9999	0.1525	1.1278
PHE: RI (4 : 1)	y = 6.5667 + 1.8593x	0.9851	0.1437	1.1905
PHE: TRI (1 : 2)	y = 6.1903 + 1.6405x	0.9944	0.1881	0.9570
PHE: TRI (1 : 3)	y = 5.9124 + 1.1673x	0.9983	0.1653	1.0993
PHE: TRI (1 : 4)	y = 6.2109 + 1.5042x	0.9847	0.1567	1.1662

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