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灭菌唑与氰烯菌酯复配对水稻恶苗病菌的抑制活性

李美霞 陈香华 周长勇 陈亚丽 朱春华 曹凯歌 王建新 周明国 段亚冰

李美霞, 陈香华, 周长勇, 陈亚丽, 朱春华, 曹凯歌, 王建新, 周明国, 段亚冰. 灭菌唑与氰烯菌酯复配对水稻恶苗病菌的抑制活性[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0134
引用本文: 李美霞, 陈香华, 周长勇, 陈亚丽, 朱春华, 曹凯歌, 王建新, 周明国, 段亚冰. 灭菌唑与氰烯菌酯复配对水稻恶苗病菌的抑制活性[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2022.0134
LI Meixia, CHEN Xianghua, ZHOU Changyong, CHEN Yali, ZHU Chunhua, CAO Kaige, WANG Jianxin, ZHOU Mingguo, DUAN Yabing. Antifungal activity study of triticonazole with phenamacril on Fusarium fujikuroi[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0134
Citation: LI Meixia, CHEN Xianghua, ZHOU Changyong, CHEN Yali, ZHU Chunhua, CAO Kaige, WANG Jianxin, ZHOU Mingguo, DUAN Yabing. Antifungal activity study of triticonazole with phenamacril on Fusarium fujikuroi[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2022.0134

灭菌唑与氰烯菌酯复配对水稻恶苗病菌的抑制活性

doi: 10.16801/j.issn.1008-7303.2022.0134
基金项目: 国家重点研发计划 (2016YFD0200503-04);南京六合区2021年基层农技推广体系改革与建设补助项目.
详细信息
    作者简介:

    李美霞,meixia729814@163.com

    通讯作者:

    段亚冰,dyb@njau.edu.cn

  • 中图分类号: S482.2;TQ450.15;S435.1

Antifungal activity study of triticonazole with phenamacril on Fusarium fujikuroi

Funds: National Key R & D Program of China (2016YFD0200503-04); 2021 Grassroots Agricultural Technology Promotion System Reform and Construction Subsidy Project in Nanjing Liuhe District.
  • 摘要: 采用菌丝生长速率法,测定了灭菌唑对水稻恶苗病菌的毒力,测得灭菌唑对10株水稻恶苗病菌的EC50值范围为0.0664~0.7661 μg/mL。采用有效成分质量浓度分别为60、120和240 μg/mL的25 g/L灭菌唑种子处理悬浮剂处理水稻种子后,对水稻发芽率、株高、鲜重均无影响。表明灭菌唑对水稻恶苗病菌具有较好的抑制活性,且对水稻安全,可用于水稻恶苗病的化学防控。氰烯菌酯对10株水稻恶苗病菌的EC50值范围为0.0076~0.2629 μg/mL。将氰烯菌酯和灭菌唑分别按质量比4 : 1、3 : 1、2 : 1、1 : 1、1 : 2、1 : 3和1 : 4复配,测定了复配药剂对水稻恶苗病菌的联合毒力,所得增效系数在0.5~1.5之间,均为相加作用。研究表明,将氰烯菌酯与灭菌唑复配后施用,不仅能显著降低单剂的使用剂量,且能降低氰烯菌酯对病原菌群体的选择压力,延缓抗药性的发生和发展速度,保障病害防效和水稻生产安全。
  • 表  1  水稻恶苗病菌敏感性测定试验中氰烯菌酯和灭菌唑复配药剂的浓度梯度

    Table  1.   Concentration gradients of sensitivity tests of F. fujikuroi to the combinations of phenamacril and triticonazole

    药剂处理
    Fungicide treatment
    浓度梯度
    Concentration gradient/ (μg/mL)
    氰烯菌酯
    phenamacril (PHE)
    0,0.0625,0.125,0.25,0.5,1
    灭菌唑
    triticonazole (TRI)
    0,0.039,0.078,0.156,0.3125,0.625,1.25
    PHE : TRI (4 : 1) 0, 0.078,0.1556,0.3125,0.625,1.25
    PHE : TRI (3 : 1) 0, 0.0125,0.025,0.05,0.1,0.2,0.4
    PHE : TRI (2 : 1) 0, 0.05,0.1,0.2,0.4,0.8
    PHE : TRI (1 : 1) 0, 0.05,0.1,0.2,0.4,0.8
    PHE : TRI (1 : 2) 0, 0.075,0.15,0.3,0.6
    PHE : TRI (1 : 3) 0, 0.039,0.078,0.156,0.3125,0.625
    PHE : TRI (1 : 4) 0, 0.0125,0.025,0.05,0.1,0.2,0.4
    下载: 导出CSV

    表  2  水稻恶苗病菌对灭菌唑的敏感性

    Table  2.   The sensitivity of F. fujikuroi to triticonazole

    菌株
    Strains
    回归方程
    Regression equation
    相关系数
    r
    有效抑制中浓度
    EC50/(μg/mL)
    ZJSX-1y = 5.6921 + 1.2144x0.99730.2692
    ZJSX-4y = 5.9860 + 1.2590x0.99820.1648
    JSHA-6y = 5.7927 + 0.9376x0.98230.1427
    JSHA-8y = 5.3572 + 1.0823x0.99490.4677
    JSHA-11y = 5.3647 + 1.0261x0.99790.4411
    JSHA-13y = 5.5402 + 1.4438x0.99340.4225
    JSTZ-14y = 5.7115 + 0.7734x0.99270.1181
    JSTZ-16y = 5.8591 + 1.0077x0.99570.1404
    JSTZ-17y = 5.1417 + 1.2248x0.99390.7661
    JSTZ-20y = 6.4429 + 1.2250x0.99340.0664
    注:ZJSX-,菌株采自浙江省绍兴地区;JSHA-,菌株采自江苏省淮安地区;JSTZ-,菌株采自江苏省泰州地区。Note: ZJSX-, the strains were collected from Shaoxing area in Zhejiang Province. JSHA-, the strains were collected from Huai'an area in Jiangsu Province. JSTZ-, the strains were collected from Taizhou area in Jiangsu Province.
    下载: 导出CSV

    表  3  25 g/L灭菌唑种子处理悬浮剂对水稻的安全性

    Table  3.   Safety tests of 25 g/L triticonazole FS on rice

    药剂
    Fungicide
    药剂质量浓度
    Mass conc. of fungicide/
    (μg/mL)
    剂量
    Dosage, a.i./
    (g/100 kg(seed))
    发芽率
    Germination rate/%
    株高
    Height/cm
    鲜重
    Weight/g
    南粳5055
    Nanjing 5055
    苏御糯
    Suyunuo
    南粳5055
    Nanjing 5055
    苏御糯
    Suyunuo
    南粳5055
    Nanjing 5055
    苏御糯
    Suyunuo
    25 g/L灭菌唑种子处理悬浮剂
    triticonazole 25 g/L FS
    0 0 87.33a 85.47a 38.79a 58.15a 0.6233a 1.0600a
    60 6 88.89a 87.18a 38.64a 58.06a 0.6067a 1.0433a
    120 12 84.13a 88.03a 38.05a 57.37a 0.5000a 1.0233a
    240 24 85.95a 85.47a 37.52a 57.12a 0.4833a 1.0133a
    注:同列数据后相同小写字母表示差异不显著(P<0.05)。Note: Same lowercase letters indicate no significant difference among the same column data (P<0.05).
    下载: 导出CSV

    表  4  水稻恶苗病菌对氰烯菌酯的敏感性

    Table  4.   Sensitivity tests of F. fujikuroi to phenamacril

    菌株
    Strains
    回归方程
    Regression equation
    相关系数
    r
    有效抑制中浓度
    EC50/(μg/mL)
    ZJSX-5y = 6.3319 + 0.9324x0.99970.0373
    ZJSX-6y = 5.9331 + 1.0859x0.99460.1383
    ZJSX-9y = 6.0792 + 0.8955x0.99320.0623
    ZJSX-11y = 6.1082 + 1.3201x0.99320.1447
    JSHA-13y = 6.3157 + 1.2705x0.99840.0921
    JSHA-16y = 6.4327 + 1.2801x0.99910.0076
    JSTZ-17y = 5.9844 + 1.0193x0.99430.1082
    JSTZ-18y = 5.6939 + 1.1958x0.99320.2629
    JSTZ-21y = 6.3412 + 1.2273x0.99660.0807
    JSTZ-22y = 6.3151 + 0.8375x0.99460.0269
    JSTZ-28y = 6.3159 + 1.4315x0.99030.1204
    注:ZJSX-,菌株采自浙江省绍兴地区;JSHA-,菌株采自江苏省淮安地区;JSTZ-,菌株采自江苏省泰州地区。Note: ZJSX-, the strains were collected from Shaoxing area in Zhejiang Province. JSHA-, the strains were collected from Huai'an area in Jiangsu Province. JSTZ-, the strains were collected from Taizhou area in Jiangsu Province.
    下载: 导出CSV

    表  5  水稻恶苗病菌对氰烯菌酯和灭菌唑各复配药剂的敏感性

    Table  5.   Synergistic effects of phenamacril and triticonazole on mycelial growth of F. fujikuroi

    药剂处理
    Fungicide treatment
    回归方程
    Regression equation
    相关
    系数
    r
    有效抑制中
    浓度
    EC50/
    (μg/mL)
    增效比
    SR
    氰烯菌酯
    phenamacril (PHE)
    y = 7.8179 + 3.6309 x0.99140.1675
    灭菌唑
    triticonazole (TRI)
    y = 5.8103 + 1.1127 x0.99950.1870
    PHE: TRI (1 : 1)y = 6.3191 + 1.9450 x0.99910.20980.8423
    PHE: TRI (2 : 1)y = 6.3484 + 1.7800 x0.99220.17480.9927
    PHE: TRI (3 : 1)y = 6.6721 + 2.0427 x0.99990.15251.1278
    PHE: RI (4 : 1)y = 6.5667 + 1.8593 x0.98510.14371.1905
    PHE: TRI (1 : 2)y = 6.1903 + 1.6405 x0.99440.18810.9570
    PHE: TRI (1 : 3)y = 5.9124 + 1.1673 x0.99830.16531.0993
    PHE: TRI (1 : 4)y = 6.2109 + 1.5042 x0.98470.15671.1662
    下载: 导出CSV
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  • 收稿日期:  2021-11-08
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