山东省不同地区棉蚜对新烟碱类杀虫剂的抗药性检测及酶抑制剂的增效作用研究

张国福; 李琳琳; 李本杰; 王开运; 夏晓明

doi:10.3969/j.issn.1008-7303.2014.06.07

山东省不同地区棉蚜对新烟碱类杀虫剂的抗药性检测及酶抑制剂的增效作用研究

doi: 10.3969/j.issn.1008-7303.2014.06.07

1.
山东农业大学植物保护学院, 山东泰安 271018

基金项目:

国家自然科学基金项目(31301695);山东省优秀中青年科学家科研奖励基金项目(BS2012SW015);山东省高等学校科技计划项目(J13LE18).

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出版历程
- 收稿日期: 2014-06-18
- 修回日期: 2014-07-29
- 刊出日期: 2014-12-12

Resistance detection and synergism of enzyme inhibitors on neonicotinoids to Aphis gossypii in Shandong Province

1.
College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong Province, China

摘要

摘要: 为明确山东省棉蚜对新烟碱类杀虫剂的抗性水平,采用毛细管微量点滴法测定了泰安、聊城和东营3个田间种群及1个敏感种群对吡虫啉、烯啶虫胺、啶虫脒、噻虫嗪、噻虫啉、噻虫胺6种新烟碱类杀虫剂的敏感性,同时测定了磷酸三苯酯(TPP)、顺丁烯二酸二乙酯(DEM)和增效醚(PBO) 3种酶抑制剂的增效作用。结果表明:泰安棉蚜种群对烯啶虫胺的抗性倍数为16.95,处于中等抗性水平,对吡虫啉和啶虫脒的抗性倍数分别为5.69和9.57,已产生低水平抗性,对噻虫胺、噻虫嗪和噻虫啉的抗性倍数均小于3.0,仍较敏感;聊城棉蚜种群对吡虫啉、啶虫脒和噻虫嗪的抗性倍数分别为28.51、25.88和18.16,属中等抗性水平,对噻虫啉和噻虫胺的抗性倍数分别为6.01和6.37,已产生低水平抗性,对烯啶虫胺仍处于敏感阶段;东营棉蚜种群对吡虫啉、啶虫脒和噻虫胺的抗性倍数分别为37.95、21.52和12.95,已产生中等水平抗性,对噻虫啉、烯啶虫胺和噻虫嗪的抗性倍数分别为7.07、6.38和4.75,处于低水平抗性阶段。多功能氧化酶抑制剂PBO和羧酸酯酶抑制剂TPP对6种供试新烟碱类杀虫剂的增效作用明显,谷胱甘肽-S-转移酶抑制剂DEM对这6种药剂也具有一定的增效作用。研究表明,山东省泰安等3地区棉蚜种群对6种新烟碱类杀虫剂均产生了不同程度的抗药性,多功能氧化酶和羧酸酯酶可能在棉蚜对该类杀虫剂的抗性中起主要作用,谷胱甘肽-S-转移酶可能也具有一定的作用。
- 棉蚜 /
- 新烟碱类杀虫剂 /
- 抗药性 /
- 酶抑制剂 /
- 增效作用
Abstract: In order to survey the resistance level of cotton aphid(Aphis gossypii Glover) to neonicotinoids in Shandong Province, the sensitivity of three field populations collected from Liaocheng, Tai'an and Dongying and one susceptible population to 6 neonicotinoids (imidacloprid, nitenpyram, acetamiprid, thamethoxam, thiacloprid, and clothianidin) was determined by capillary micro-topical application method. The synergisms of three enzyme inhibitors(TPP, DEM and PBO) on the toxicity of six neonicotinoids to A.gossypii were also measured. The results showed that Tai'an population of A.gossypii exhibited medium level of resistance to nitenpyram, with resistance ratio of 16.95-fold; low level of resistance to imidacloprid and acetamiprid, with resistance ratio of 5.69- and 9.57-fold, respectively; but no resistance to clothianidin, thamethoxam and thiacloprid, with resistance ratio less than 3.0-fold. Liaocheng population of A.gossypii exhibited medium level of resistance to imidacloprid, acetamiprid and thamethoxam, with resistance ratio of 28.51-, 25.88- and 18.16-fold, respectively; and low level of resistance to thiacloprid and clothianidin, with resistance ratio of 6.01- and 6.37-fold, respectively; but still sensitive to nitenpyram. Dongying polulation of A.gossypii exhibited medium level of resistance to imidacloprid, acetamiprid and clothianidin, with resistance ratio of 37.95-, 21.52- and 12.95-fold, respectively; and low level of resistance to clothianidin, nitenpyram and thamethoxam, with resistance ratio ranged from 4.75- to 7.07-fold. Enzyme inhibitor PBO and TPP had significant synergism on the 6 neonicotinoids; DEM also showed synergism to some extent. In conclusion, the results of present study suggested that the field populations of cotton aphid collected from three areas of Shandong province developed different level of resistance to the six neonicotinoids tested; the detoxification of mixed-functional oxidase (MFO), carboxylesterase (CarE) might play a key role in the resistance; glutathione-S-transferase (GST) might also make a certain contribution to the resisitance
- Aphis gossypii /
- neonicotinoid insecticides /
- resistance /
- enzyme inhibitor /
- synergism

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